Model-based clustering of Escherichia coli O157:H7 genotypes and their potential association with clinical outcome in human infections.
Abstract
This study addresses the potential association of Escherichia coli O157:H7 genetic clusters with severe clinical manifestations in humans. The genotypes used in this model-based clustering had been delineated on the basis of lineage-specific polymorphism assay, Shiga toxin-encoding bacteriophage insertion site assay, clade typing, tir (A255T) polymorphism, variant analysis of Shiga toxin 2 gene, and antiterminator Q genes. Based on this model, the distribution of genotypes among tested strains suggested the presence of 6 main genetic clusters of E. coli O157:H7 strains. Clusters 1 and 3 were observed to be more frequent among E. coli O157:H7 strains isolated from bloody diarrhea and hemolytic uremic syndrome, respectively. Consequently, our findings supported the growing evidence of the existence of distinct genotypes of E. coli O157:H7 that differ in their virulence levels to human.
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. Electronic address: mm_elhadidy@mans.edu.eg.Department of Microbiology & Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St. Abbassia, Cairo 11566, Egypt; Department of Pharmacy Practice, School of Pharmacy, Hampton University, Kittrell Hall Hampton, VA 23668, USA.Department of Microbiology, Belgian STEC/VTEC National Reference Centre for Human Microbiology, University Hospital Brussels (UZ Brussel), Laarbeeklaan, Belgium.Technology and Food Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Brusselsesteenweg 370, Melle 9090, Belgium.Technology and Food Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Brusselsesteenweg 370, Melle 9090, Belgium; Department of Pathology, Bacteriology and Poultry Diseases, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium. MeSH
Cluster AnalysisDiarrheaEscherichia coli InfectionsEscherichia coli O157Genes, BacterialGenotypeHemolytic-Uremic SyndromeHumansMolecular EpidemiologyMolecular TypingTreatment Outcome
Pub Type(s)
Journal Article
Research Support, Non-U.S. Gov't
