The increasing demands for portable, wearable, and implantable sensing devices have stimulated growing interest in innovative electrode materials. In this work, we have demonstrated that printing a conductive ink formulated by blending three-dimensional (3D) porous graphene-carbon nanotube (CNT) assembly with ionic liquid (IL) on two-dimensional (2D) graphene paper (GP), leads to a freestanding GP supported graphene-CNT-IL nanocomposite (graphene-CNT-IL/GP). The incorporation of highly conductive CNTs into graphene assembly effectively increases its surface area and improves its electrical and mechanical properties. The graphene-CNT-IL/GP, as freestanding and flexible substrates, allows for efficient loading of PtAu alloy nanoparticles by means of ultrasonic-electrochemical deposition. Owing to the synergistic effect of PtAu alloy nanoparticles, 3D porous graphene-CNT scaffold, IL binder and 2D flexible GP substrate, the resultant lightweight nanohybrid paper electrode exhibits excellent sensing performances in nonenzymatic electrochemical detection of glucose in terms of sensitivity, selectivity, reproducibility and mechanical properties.