The polyclonal mono-specific sera to proteins of toxin and antigens of S-layer of Bacillus Anthracis separated in preparative electrophoresis. While fluorescent antibody technique was applied, immunoglobulins of sera to protein of S-layer of Bacillus Anthracis m.m. 94 kDa had specificity and specific activity in presented number of strains. In the soil samples spores in concentration of 1x104 KOE/ml were detected that permits to recommend the given immunoglobulins for indexation of Bacillus Anthracis. The proteins m.m. 90 kDa relevant to antigens of toxin of Bacillus Anthracis reacted in immuno-blotting with sera from patients with skin form of anthrax and guinea pigs immunized and survived after infection. Thew sera to them are species specified and can be applied for diagnostic of disease (detection of protective antigen) and detection of production of proteins in reaction of immunodiffusion with growing cultures.