A second Ewing's sarcoma translocation, t(21;22), fuses the EWS gene to another ETS-family transcription factor, ERG.
Abstract
The t(11;22)(q24;q12), present in 85% of Ewing's sarcoma and related tumours, fuses the EWS gene from chromosome 22q12 and the ETS family member, FLI-1. This results in the expression of a chimaeric protein containing the amino-terminal portion of EWS fused to the ETS DNA-binding domain of FLI-1. We have identified a second Ewing's sarcoma translocation, t(21;22)(q22;q12), that fuses EWS to a different ETS family member, the ERG gene located on band 21q22. Identical EWS nucleotide sequences found in the EWS/FLI-1 fusion transcripts are fused to portions of ERG encoding an ETS DNA-binding domain resulting in expression of a hybrid EWS/ERG protein. These findings suggest that fusion of EWS to different members of the ETS family of transcription factor genes may result in the expression of similar disease phenotypes.
Department of Pathology and Laboratory Medicine, Childrens Hospital of Los Angeles/University of Southern California 90027.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available MeSH
Amino Acid SequenceBase SequenceChildChild, PreschoolChromosomes, Human, Pair 21Chromosomes, Human, Pair 22Cloning, MolecularDNA-Binding ProteinsEarly Growth Response Protein 1FemaleHumansImmediate-Early ProteinsKaryotypingMolecular Sequence DataPrecipitin TestsProto-Oncogene Protein c-fli-1Proto-Oncogene ProteinsRNA, MessengerRecombinant Fusion ProteinsRetroviridae Proteins, OncogenicSarcoma, EwingSequence Analysis, DNATrans-ActivatorsTranscription FactorsTranscription, GeneticTranslocation, GeneticTumor Cells, Cultured
Pub Type(s)
Journal Article
Research Support, Non-U.S. Gov't