Tumor necrosis factor receptor p55 mediates induction of HIV type 1 expression in chronically infected U1 cells.
AIDS Res Hum Retroviruses. 1996 Feb 10; 12(3):199-204.AR

Abstract

Tumor necrosis factor alpha (TNF-alpha) is a potent inducer of human immunodeficiency virus type 1 (HIV-1) expression in chronically infected cells. The aim of this study was to investigate the role played by the two known TNF-alpha receptors, TNFR-p55 and TNFR-p75, in the activation of HIV-1 expression. As a model system the latently infected human promonocytic cell line U1 was stimulated with wild-type TNF-alpha, with TNF-alpha muteins that specifically bind to one or the other receptor or with receptor-specific monoclonal antibodies. Induction of HIV-1 expression, measured by p24 core antigen capture enzyme-linked immunosorbent assay (ELISA), was found to be exclusively triggered by TNFR-p55 stimulation. However, our results also showed that the addition of TNFR-p75-specific ligands negatively modulated the HIV-1 expression induced via TNFR-p55.

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Authors+Show Affiliations

Edfjäll C
Pharmaceutical Research Gene Technologies, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Jacobsen H
No affiliation info available
Lötscher H
No affiliation info available
Mous J
No affiliation info available

MeSH

Antigens, CDCell LineHIV Core Protein p24HIV-1HumansMonocytesReceptors, Tumor Necrosis FactorReceptors, Tumor Necrosis Factor, Type IReceptors, Tumor Necrosis Factor, Type IITumor Necrosis Factor-alpha

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8835197