- Discovery and characterization of hydroxylysine O-glycosylation in an engineered IL-2 fusion protein. [Journal Article]Protein Expr Purif. 2023 Feb 01 [Online ahead of print]PE
- In the present study, an engineered interleukin-2 (IL-2) fusion protein consisting of an anti-human serum albumin nanobody linked by ASTKG and a (G4S)2 linker to IL-2 was constructed. Liquid chromatography-mass spectrometry (LC-MS) characterization was performed on the intact molecule and at the peptide level. The liquid chromatography-mass spectrometry molecular mass analysis for the engineered …
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- Label-free biosensing of lignans for therapeutics using engineered model surfaces. [Journal Article]Int J Biol Macromol. 2023 Feb 01 [Online ahead of print]IJ
- The label-free interaction analysis of macromolecules and small molecules has increasing importance nowadays, both in diagnostics and therapeutics. In the blood vascular system, human serum albumin (HSA) is a vital globular transport protein with potential multiple ligand binding sites. Characterizing the binding affinity of compounds to HSA is essential in pharmaceutics and in developing new com…
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- Penetration of Perfluorooctanesulfonate Isomers and Their Alternatives from Maternal Blood to Milk and Its Associations with Chemical Properties and Milk Primary Components. [Journal Article]Environ Sci Technol. 2023 Feb 02 [Online ahead of print]ES
- Perfluorooctanesulfonate (PFOS) and its alternatives, including chlorinated polyfluorinated ether sulfonates (Cl-PFESAs), are mainly detected per- and polyfluoroalkyl substances (PFAS) in human samples such as milk. However, the mechanism of their blood to milk transfer was not well studied. Here, 145 paired maternal serum and human milk samples were analyzed for six PFOS isomers and Cl-PFESAs to…
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- Exploring novel fluorine-rich fuberidazole derivatives as hypoxic cancer inhibitors: Design, synthesis, pharmacokinetics, molecular docking, and DFT evaluations. [Journal Article]PLoS One. 2023; 18(2):e0262790.Plos
- Sixteen fuberidazole derivatives as potential new anticancer bioreductive prodrugs were prepared and characterized. The in vitro anticancer potential was examined to explore their cytotoxic properties by employing apoptosis, DNA damage, and proliferation tests on chosen hypoxic cancer cells. Eight substances (Compound 5a, 5c, 5d, 5e, 5g, 5h, 5i, and 5m) showed promising cytotoxicity values compar…
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- Short-term hepatocyte function and portal hypertension outcomes of sofosbuvir/velpatasvir for decompensated hepatitis C-related cirrhosis. [Journal Article]J Gastroenterol. 2023 Feb 02 [Online ahead of print]JG
- CONCLUSIONS: Achieving SVR24 with SOF/VEL treatment in patients with decompensated hepatitis C-related cirrhosis can be expected to improve hepatocyte function and portal hypertension on short-term follow-up.
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- Autoxidation of ascorbate mediates lysine N-pyrrolation. [Journal Article]Free Radic Res. 2023 Feb 01 [Online ahead of print]FR
- Protein N-pyrrolation, which converts lysine residues to Nε-pyrrole-L-lysine (pyrK), is a naturally-occurring covalent modification. The pyrrolated proteins have a unique property of binding to DNA-staining agessnts, such as SYBR Green I (SG), and anti-DNA antibodies, suggesting a physiologically relevant modification that gives rise to DNA mimic protein. These properties of pyrrolated protein ar…
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- Noninvasive, continuous fluorescence monitoring of bilirubin photodegradation. [Review]Phys Chem Chem Phys. 2023 Jan 27 [Online ahead of print]PC
- Nowadays phototherapy is widely used for treatment of various diseases. However, efficient application of phototherapy requires an understanding of light interactions with main endogenous chromophores (e.g., hemoglobin, bilirubin, and water) in tissue. In particular, bilirubin is the target chromophore in the treatment of neonatal jaundice, which is the most common disease affecting up to 80% of …
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- Efficacy of Intravenous Albumin for Spontaneous Bacterial Peritonitis Infection Among Patients With Cirrhosis: A Meta-Analysis of Randomized Control Trials. [Review]
- Albumin is an important component in the standard therapeutic approach to spontaneous bacterial peritonitis (SBP). This meta-analysis aimed to determine the impact of intravenous human albumin in patients with cirrhosis and SBP. This study was conducted according to the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Two reviewers independently searc…
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- Novel Self-Assembled Multifunctional Nanoprobes for Second-Near-Infrared-Fluorescence-Image-Guided Breast Cancer Surgery and Enhanced Radiotherapy Efficacy. [Journal Article]Adv Sci (Weinh). 2023 Jan 31 [Online ahead of print]AS
- Breast-conserving surgery (BCS) is the predominant treatment approach for initial breast cancer. However, due to a lack of effective methods evaluating BCS margins, local recurrence caused by positive margins remains an issue. Accordingly, radiation therapy (RT) is a common modality in patients with advanced breast cancer. However, while RT also protects normal tissue and enhances tumor bed doses…
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- In-situ synchrotron quantitative analysis of competitive adsorption tendency of human serum proteins on polyether sulfone clinical hemodialysis membrane. [Journal Article]
- Comprehensive understanding of protein adsorption phenomenon on membrane surface during hemodialysis (HD) is one of the key moments for development of hemocompatible HD membrane. Though many mechanisms and kinetics of protein adsorption on some surface have been studied, we are still far away from complete understanding and control of this process, which results in a series of biochemical reactio…
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- Dysregulation of metalloproteins in ischemic heart disease patients with systolic dysfunction. [Journal Article]Int J Biol Macromol. 2023 Jan 28; 232:123435.IJ
- Ischemic heart disease (IHD) is the leading cause of mortality worldwide. Metalloproteins have been linked to human health and diseases. The molecular functions of metalloproteins in IHD is not well understood and require further exploration. The objective of this study was to find out the role of metalloproteins in the pericardial fluid of IHD patients having normal (EF > 45) and impaired (EF < …
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- Closed-loop vasculature network design for bioprinting large, solid tissue scaffolds. [Journal Article]Biofabrication. 2023 Jan 30 [Online ahead of print]B
- Vascularization is an indispensable requirement for fabricating large solid tissues and organs. The natural vasculature derived from medical imaging modalities for large tissues and organs are highly complex and convoluted. However, the present bioprinting capabilities limit the fabrication of such complex natural vascular networks. Simplified bioprinted vascular networks, on the other hand, lack…
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- Lung-Targeted Transgene Expression of Nanocomplexed Ad5 Enhances Immune Response in the Presence of Preexisting Immunity. [Journal Article]
- Recombinant adenovirus serotype 5 (Ad5) vector has been widely applied in vaccine development targeting infectious diseases, such as Ebola virus disease and coronavirus disease 2019 (COVID-19). However, the high prevalence of preexisting anti-vector immunity compromises the immunogenicity of Ad5-based vaccines. Thus, there is a substantial unmet need to minimize preexisting immunity while improvi…
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- Bone microenvironment regulative hydrogels with ROS scavenging and prolonged oxygen-generating for enhancing bone repair. [Journal Article]
- Large bone defects resulting from fractures and disease are a major clinical challenge, being often unable to heal spontaneously by the body's repair mechanisms. Lines of evidence have shown that hypoxia-induced overproduction of ROS in bone defect region has a major impact on delaying bone regeneration. However, replenishing excess oxygen in a short time cause high oxygen tension that affect the…
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- Silver Nanoparticle Surface Chemistry Determines Interactions with Human Serum Albumin and Cytotoxic Responses in Human Liver Cells. [Journal Article]
- Engineered nanomaterials (ENMs) are synthesized with a diversity of surface chemistries that mediate biochemical interactions and physiological response to the particles. In this work, silver engineered nanomaterials (AgENMs) are used to evaluate the role of surface charge in protein interactions and cellular cytotoxicity. The most abundant protein in blood, human serum albumin (HSA), was interac…
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- Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection. [Journal Article]
- A factor closely associated with renal disease status in clinical diagnosis is abnormal human serum albumin (HSA) concentration levels in human body fluids urine, serum, etc. The surface stress biosensor was developed as a new type of biosensor to detect protein molecule concentration and has a wide range of clinical applications. However, further sensitivity improvement is required to achieve hi…
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- Optimizing a fully automated and closed system process for red blood cell reduction of human bone marrow products. [Journal Article]Cytotherapy. 2023 Jan 27 [Online ahead of print]C
- CONCLUSIONS: The fully automated and closed SmartRedux program on the Sepax was shown to be effective at reducing RBCs from "bone marrow-like" products and a supplemented bone marrow product using a 10× reduction rate.
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- Binding elucidation of azo dyes with human serum albumin via spectroscopic approaches and molecular docking techniques. [Journal Article]J Biomol Struct Dyn. 2023 Jan 29 [Online ahead of print]JB
- The large number of synthesized azo dyes is widely applied in the food, pharmaceutical, cosmetic, textile, and leather industries. In this study, the binding mechanism of two synthesized dyes with human serum albumin (HSA); as the most abundant protein in plasma; was elucidated by fluorescence spectroscopy, Fourier-transform infrared spectroscopy and molecular modeling methods. The fluorescence q…
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- Interaction of polystyrene nanoparticles with supported lipid bilayers: Impact of nanoparticle size and protein corona. [Journal Article]Macromol Biosci. 2023 Jan 27 [Online ahead of print]MB
- Polystyrene is one of the most widely used plastics. Here we report on the interaction of 50 nm- and 210 nm polystyrene nanoparticles (PSNPs) with human serum albumin and transferrin, as well as their effect on supported lipid bilayers (SLBs) using both experimental and theoretical approaches. We found by dynamic light scattering and atomic force microscopy measurements that the increase in the d…
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- A comprehensive evaluation of stable expression "hot spot" in the ScltI gene of Chinese hamster ovary cells. [Journal Article]Appl Microbiol Biotechnol. 2023 Feb; 107(4):1299-1309.AM
- The Chinese hamster ovary (CHO) cell is the most widely used biopharmaceutical expression system, but its long-term expression is unstable. This issue can be effectively addressed by site-specific integration of exogenous genes into the genome. Therefore, exogenous protein sites with stable expression in the CHO cell genome must be identified. CRISPR/Cas9 technology was used in this study to inte…
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- Inflammatory biomarkers behavior profile among patients following elective degenerative spine surgery and their differences with those coursing with a postoperative spinal infection: Protocol for a Systematic Review. [Journal Article]JMIR Res Protoc. 2023 Jan 26 [Online ahead of print]JR
- CONCLUSIONS: The present study will provide the biomarkers' behavior profile for PSI and patients following elective surgery for degenerative spinal diseases from the preoperative period up to 90 days postoperative providing cut-off values at the day of diagnosis. It will provide clinicians with a better background to diagnose based on highly trustable information based on cut-off values from this systematic review. Finally we expect this review to provide a basis for future research on biomarkers to establish more accurate and timely diagnosis in the early stages of illness ultimately impacting the patient's physical and mental health, reducing the disease burden.
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- Explanation of inconsistencies in the determination of human serum albumin thermal stability. [Journal Article]Int J Biol Macromol. 2023 Jan 23; 232:123379.IJ
- Thermal denaturation of human serum albumin has been the subject of many studies in recent decades, but the results of these studies are often conflicting and inconclusive. To clarify this, we combined different spectroscopic and calorimetric techniques and performed an in-depth analysis of the structural changes that occur during the thermal unfolding of different conformational forms of human s…
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- Experimental and computational studies of silver(I) dibenzoylmethane-based complexes, interaction with DNA/RNA/BSA biomolecules, and in vitro cytotoxic activity. [Journal Article]J Inorg Biochem. 2023 Jan 20; 241:112132.JI
- Two silver(I) complexes of composition [Ag2(L)2] (1) and [Ag(L)(PPh3)2](2) (HL = dibenzoyl- methane, PPh3 = triphenylphosphine) were synthesized and characterized by elemental analysis, FTIR, NMR, XRPD, and UV-visible spectra. The molecular structures of the studied ligands and Ag(I) complexes have been characterized using Density Function Theory (DFT) calculations. This analysis has enabled us t…
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- Partially replacing cyclophosphamide with bendamustine in combination with cyclosporine A improves survival and reduces xenogeneic graft-versus-host-disease. [Journal Article]
- CONCLUSIONS: Together, we illustrate that the use of CY/BEN is safe and shows similar control of xGvHD to CY/CY, but when combined with CSA, survival with CY/BEN is significantly prolonged compared to CY/CY.
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- Contrast-enhanced Ultrasound Using Intradermal Microbubble Sulfur Hexafluoride for Identification of Sentinel Lymph Nodes During Breast Cancer Surgery: A Clinical Trial. [Clinical Trial, Phase III]Anticancer Res. 2023 Feb; 43(2):557-567.AR
- CONCLUSIONS: CEUS is a safe and manageable intraoperative procedure. When compared with standard techniques, US appearance during CEUS may provide additional information when associated with histological assessment.
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- Albumin-based drug carrier targeting urokinase receptor for cancer therapy. [Journal Article]Int J Pharm. 2023 Jan 22; 634:122636.IJ
- Urokinase plasminogen activator receptor (uPAR) is a key participant in extracellular proteolysis, tissue remodeling and cell motility. uPAR overexpresses in most solid tumors and several hematologic malignancies, but has low levels on normal tissues, thus is advocated as a molecular target for cancer therapy. One of the obstacles for the evaluation of uPAR targeting agents in preclinical study i…
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- Modulation of Neutrophil Function by Recombinant Human IgG1 Fc Hexamer in the Endogenous K/BxN Mouse Model of Rheumatoid Arthritis. [Journal Article]Pharmacology. 2023 Jan 25 [Online ahead of print]P
- CONCLUSIONS: In the endogenous K/BxN mouse model of rheumatoid arthritis, amelioration can be explained in part by inhibition of neutrophil infiltration into the joints as well as inhibition of IL-1β production. Given the observed inhibitory properties on neutrophils, rFc-µTP-L309C may be a potential therapeutic candidate to treat autoimmune and inflammatory conditions in which neutrophils are the predominant cell type involved in pathogenesis.
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- Supramolecular Phenylalanine-Derived Hydrogels for the Sustained Release of Functional Proteins. [Journal Article]ACS Biomater Sci Eng. 2023 Jan 24 [Online ahead of print]AB
- Protein-based therapeutics have emerged as next-generation pharmaceutical agents for oncology, bone regeneration, autoimmune disorders, viral infections, and other diseases. The clinical application of protein therapeutics has been impeded by pharmacokinetic and pharmacodynamic challenges including off-target toxicity, rapid clearance, and drug stability. Strategies for the localized and sustaine…
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- Barrier-penetrating liposome targeted delivery of basic fibroblast growth factor for spinal cord injury repair. [Journal Article]
- Nanoparticle technologies offer a non-invasive means to deliver basic fibroblast growth factor (bFGF) for the treatment of spinal cord injury (SCI). However, the inability of bFGF to accumulate at the injury site and inefficient penetration across the blood-spinal cord barrier (BSCB) remain challenges. The present study describes a dual-targeting liposome (bFGF@Lip-Cp&Rp) with injury lesion targe…
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- Albumin-fused long-acting FGF21 analogue for the treatment of non-alcoholic fatty liver disease. [Journal Article]J Control Release. 2023 Jan 20 [Online ahead of print]JC
- Non-alcoholic fatty liver disease (NAFLD) currently affects about 25% of the world's population, and the numbers continue to rise as the number of obese patients increases. However, there are currently no approved treatments for NAFLD. This study reports on the evaluation of the therapeutic effect of a recombinant human serum albumin-fibroblast growth factor 21 analogue fusion protein (HSA-FGF21)…
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