- Impact of the anterior commissure involvement on the survival of early glottic cancer treated with cricohyoidoepiglottopexy: a retrospective study. [Journal Article]
- CMCancer Manag Res 2018; 10:5553-5558
- CONCLUSIONS: SCL with cricohyoidoepiglottopexy seems to be an adequate treatment modality, even for T1b-T2 glottic tumors with anterior commissure involvement.
- From telescope to binoculars. Dyadic outcome resulting from psychological counselling for infertile couples undergoing ART. [Journal Article]
- JRJ Reprod Infant Psychol 2018 Nov 23; :1-13
- CONCLUSIONS: The findings provide support for the effectiveness of counselling on interpersonal outcome. The CFM allows researchers to examine how the dyad as a whole responds to counselling, highlighting the change in the couple's relational dynamics.
- Indicaxanthin from Opuntia Ficus Indica (L. Mill) impairs melanoma cell proliferation, invasiveness, and tumor progression. [Journal Article]
- PPhytomedicine 2018 Nov 15; 50:19-24
- CONCLUSIONS: In conclusion, we demonstrated that indicaxanthin represents a novel phytochemical able to significantly inhibit human melanoma cell proliferation in vitro and to impair tumor progression in vivo. When considering the resistance of melanoma to the current therapeutical approach and the very limited number of phytochemicals able to partially counteract it, our findings may be of interest to explore indicaxanthin potential in further and more complex melanoma studies in combo therapy, i.e. where different check points of melanoma development are targeted.
- Treatment of DVT with rivaroxaban and its potential to prevent the post-thrombotic syndrome. [Journal Article]
- IAInt Angiol 2018 Nov 20
- CONCLUSIONS: Our results suggest that rivaroxaban could have a pro-fibrinolytic effect not only on recent thrombus but also on organized thrombus that results in a complete recanalization of affected veins. It is proposed that this lytic effect will preserve venous valve structure and lead to a reduction of incidence of post-thrombotic syndrome in rivaroxaban treated patients.
- Synergistic enantioseparation systems with either cyclodextrins or cyclofructans and L-alanine Tert butyl ester lactate. [Journal Article]
- EElectrophoresis 2018 Nov 18
- The combined use of chiral ionic liquids (CILs) and conventional chiral selectors (CSs) in CE, to establish a synergistic system, has proven to be an effective approach for the separation of enantiom...
The combined use of chiral ionic liquids (CILs) and conventional chiral selectors (CSs) in CE, to establish a synergistic system, has proven to be an effective approach for the separation of enantiomeric pairs. In this study, a new CE method was developed, employing a binary system of a CS, either a cyclodextrin (CD) or a cyclofructan (CF), and a chiral amino acid ester-based ionic liquid (AAIL), for the chiral separation of four basic, acidic and zwitterionic drug compounds. In particular, the enantioseparation of two anticoagulants, warfarin (WAR) and coumachlor (COU), a non-opioid analgesic, nefopam (NEF) and a third-generation antihistamine, fexofenadine (FXD), was examined, by supporting the BGE with a CS and the chiral AAIL L-alanine tert butyl ester lactate (L-AlaC4 Lac). Parameters, such as the type of the CS, the concentration of both the CS and L-AlaC4 Lac, and the BGE pH, were methodically examined in order to optimize the chiral separation of each analyte. It was observed that, in some cases, the addition of the AAIL into the BGE improved both resolution (Rs ) and efficiency (N) significantly. In other cases, the synergistic effect enabled baseline separation of analyte enantiomers, at a much lower concentration of the CS. Finally, after optimization of separation conditions, baseline separations (Rs >1.5) of all four analytes were achieved in less than 5 min.
- LC-MS/MS analysis of the plasma concentrations of a cocktail of 5 cytochrome P450 and P-glycoprotein probe substrates and their metabolites using subtherapeutic doses. [Journal Article]
- JPJ Pharm Biomed Anal 2018 Oct 28; 164:430-441
- Drug transporters and CYP enzymes are important sources of pharmacokinetics (PK) variability in drug responses and can cause various pharmacological and toxicological consequences, leading to either ...
Drug transporters and CYP enzymes are important sources of pharmacokinetics (PK) variability in drug responses and can cause various pharmacological and toxicological consequences, leading to either toxicity or an insufficient pharmacological effect. In recent years, the cocktail approach was developed to determine in vivo CYP and transporters activities, but these approaches are somewhat limited. We described the development and validation of three sensitive and specific LC-MS/MS assays for the determination of P-gp and major human CYP isoenzyme activities following oral administration of a drug cocktail of subtherapeutic doses (lower than 10 times) of caffeine (CAF), omeprazole (OME), losartan (LOS), midazolam (MDZ), metoprolol (METO) and fexofenadine (FEX) in healthy volunteers. The three validated methods were selective for all tested analytes. No interference or matrix effect was observed for the mass transition and retention times for all compounds monitored. Additionally, assays were linear over a wide range, and limits of quantification varied between 0.01-5 ng/mL plasma. The coefficients of variation obtained in the precision studies and the inter- and intra-assay accuracies were less than 15%, guaranteeing the reproducibility and repeatability of the results. All substrates and metabolites were stable in plasma during freeze-thaw cycles. Three healthy volunteers were selected based on genotyping for CYP2C9, CYP2C19 and CYP2D6. One volunteer was genotyped as an extensive metabolizer (EM) for all tested CYP isoforms, one volunteer was genotyped as a poor metabolizer (PM) for the CYP2C9 isoform (CYP2C9*3/*3), and one volunteer was genotyped as a PM for the CYP2D6 isoform (CYP2D6*4/*4). The methods allowed the quantification of all analytes over the entire sampling period (12 h) in all studied genotypes. Thus, the analytical methods described here were sufficiently sensitive for use in low-dose pharmacokinetic studies.
- Increased eryptosis in smokers is associated with the antioxidant status and C-reactive protein levels. [Journal Article]
- TToxicology 2018 Oct 29; 411:43-48
- Cigarette smoking has been linked with oxidative stress and inflammation. In turn, eryptosis, the suicidal erythrocyte death similar to apoptosis that can be triggered by oxidative stress, has been a...
Cigarette smoking has been linked with oxidative stress and inflammation. In turn, eryptosis, the suicidal erythrocyte death similar to apoptosis that can be triggered by oxidative stress, has been associated with chronic inflammatory diseases including atherosclerosis. However, the link between smoking and eryptosis has not been explored so far. The aim of the present study was to determine the level of eryptotic erythrocytes in healthy male smokers (n = 21) compared to non-smokers (n = 21) and assess its relationship with systemic inflammation (CRP) as well as with antioxidant defense (GSH) and their resistance to ex-vivo induced hemolysis. Smoking caused an increase in phosphatidylserine translocation outside the erythrocyte membrane (hallmark of eryptosis), significantly correlated to the plasma level of CRP (r = 0.546) and GSH concentration in erythrocytes (r=-0.475). With respect to non-smokers, smokers show a marginal increase of total leucocytes and erythrocyte volume, no modifications of the RBC resistance to oxidative stress-induced hemolysis and hematological and lipid parameters unvaried. We conclude that the inflammatory status (high CRP levels) and RBC oxidative stress (low GSH levels) caused by cigarette smoking are associated with an increase of eryptotic erythrocytes, a yet unknown relationship potentially involved with atherosclerosis and cardiovascular disease in smokers.
- Numerical prediction of ultrasonic attenuation in concentrated emulsions and suspensions using Monte Carlo method. [Journal Article]
- UUltrasonics 2018 Sep 21
- For the study of predicting ultrasonic attenuation of mixed particles and probing the effect of interaction between neighboring particles, the Monte Carlo method was investigated to establish a submi...
For the study of predicting ultrasonic attenuation of mixed particles and probing the effect of interaction between neighboring particles, the Monte Carlo method was investigated to establish a submicron particle size characterization model in concentrated particulate two-phase system and serve as a probability and statistics technique to evaluate the underlying ultrasonic events during the ultrasound propagation. The numerical simulation method was proposed to predict the ultrasonic attenuation characteristics in the two-phase system of silica suspensions and corn oil-in-water emulsions with different particle sizes, ultrasonic frequencies and concentrations. Furthermore, an extension of the well-established single-particle theory of Epstein-Carhart and Allegra-Hawley (ECAH) was carried out, by incorporated in the couple phase model from a hydrodynamic point of view and effective hypothesis both accounted for the ultrasonic wave overlapping effect for the close proximity of particles. The simulation result shows agreement with the results of the ECAH model, the Lloyd & Berry (LB) model and the Waterman model in the dilute limitation, corresponding to glass beads and silica particles respectively. Afterwards, such a method was then applied into mixed particle system, where the mixed iron particles and glass beads with various ratios were set as examples for the purpose of predicting ultrasonic attenuation for the mixed particle systems. After comparing with the experimental results, it is shown that as a function of frequency, the variation of the ultrasonic attenuation coefficient with different mixing ratio manifests a nonlinear tendency. Also noteworthy is that the physical properties of particles play a dramatic impact in influencing ultrasonic attenuation. At higher concentrations, it was validated both in two-phase system of silica suspensions and corn oil-in-water emulsions that the attenuation predicted by Monte Carlo method agreed well with the experimental results of literature, yielding a theoretically increasing but less than linear expected attenuation with particle concentration. Particularly, the critical concentration of deviation from the linear change was obtained and interpreted using the thermal and viscous overlapping theory. The proposed Monte Carlo method presents a novel approach in calculating the attenuation in high particle volume concentration of more than 40% and provides a numerical modeling of particle size measurement in the complex particle-particle interaction condition.
- Drug-drug interactions of P-gp substrates unrelated to CYP metabolism. [Journal Article]
- CDCurr Drug Metab 2018 10 03
- CONCLUSIONS: Our manuscript concludes that the PK changes of the DDIs among P-gp drugs unrelated to CYP metabolism are less likely to be serious, and it appears to be convincing that the absences of clinical effects caused to the PK changes by the P-gp inducers is predominant compared with the excessive effects caused to those by the P-gp inhibitors.
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- Valve-in-valve-in-valve with the New Valve Technology allegra transcatheter heart valve system. [Journal Article]
- EHEur Heart J 2018 Oct 01