The effects of broiler age on the apparent metabolizable energy (AME) and nitrogen-corrected AME (AMEn) of soybean meal (SBM) and canola meal (CM) were examined. A corn-SBM basal diet was developed, and two test diets were formulated by substituting (w/w) 300 g/kg of the basal diet with SBM or CM. Six groups of broiler chickens, aged 1-7, 8-14, 15-21, 22-28, 29-35 or 36-42 d post-hatch, were utilized. Each diet, in pellet form, was randomly allocated to six replicate cages in each age group. Except for the 1-7 d age group, the birds were fed a starter (d 1-21) and/or a finisher (d 22-35) diet prior to the introduction of the experimental diets. The number of birds per cage was 10 (d 1-7), 8 (d 8-14) and 6 (d 15-42). The AME and AMEn of SBM and CM were determined by total excreta collection. The data for each protein source were subjected to orthogonal polynomial contrasts using the General Linear Models procedure. Bird age decreased the retention of dry matter quadratically (p < 0.001) for both SBM and CM. The retention of nitrogen decreased linearly (p < 0.001) with the advancing age of broilers for SBM and CM. The AMEn of SBM and CM decreased quadratically (p < 0.001) as the birds grew older. The highest AMEn was observed during week 1 for both SBM and CM, then declined until week 3, followed by increases thereafter. The current results showed that the age of broiler chickens influenced the AMEn of SBM and CM and supported the use of age-dependent AMEn of feed ingredients in diet formulations.

Four new natural compounds named hericenone O (1), hericenone P (2), hericenone Q (3), and hericenone R (4), two of them were reported synthetically (3-4), together with eleven known compounds were isolated from the fruiting bodies of Hericium erinaceus. The chemical structures of the isolated compounds were elucidated by using NMR analysis and mass spectrometry, as well as comparisons with the reported data in the literature. The bioactivity evaluation revealed that hericenone Q showed significant cytotoxic activity against Hep-G2 with IC50 values of 23.89 μM, and against HCT-116 with IC50 values of 65.64 μM.

This study targets the evaluation of melanin synthesis inhibition activity of the bamboo shoot skin as agro-waste. The total methanolic extract of bamboo peel extract was evaluated for its skin protective effects via measuring its melanin inhibitory activity and its suppression activity on the expression of tyrosinase mRNA levels. Results showed that bamboo peel extract has a good ability for the inhibition of melanin synthesis so further studies were performed for the isolation of its constituents. Twelve compounds have been isolated from the shoot skin of Phyllostachys pubescens. Their structures were elucidated based on extensive spectroscopic methods. The melanin inhibition potential of the isolates was tested with their collagen-production-promoting activity for the determination of active principles. Results showed that Betulinic acid, tachioside, and 1,2-dilinolenin significantly suppressed melanin production per cell compared to control. Triacontanol, tricin, and (+)-lyoniresinol 9'-O-glucoside also tended to decrease melanin production per cell. These findings indicated that the skin of bamboo shoots, a significant agricultural waste, is a useful natural source for further research on its potential for aging problems such hyperpigmentation and cognitive function impairment.

Body composition plays an important role in reproduction in broiler breeders. The aim of this study was to evaluate the dynamics in body composition and energetic efficiency in broiler breeders, using different dietary strategies. About 1,536-day-old pullets were randomly allotted to 24 pens in a 2 × 4 factorial design with 2 growth curves (standard or elevated (+15%)) and 4 diets, with a step-wise increment in energy (96, 100, 104, and 108% apparent metabolizable energy nitrogen corrected [AMEn]) fed on a pair-gain basis. Body composition was determined at 10 time points from 0 to 60 wk of age. Body protein mass was linearly related to body weight (BW) in growing breeders, which can be expressed as -6.4+0.184*BW (R2 = 0.99; P < 0.001). Body fat mass was exponentially related to BW in growing breeders, which can be expressed as -42.2+50.8*1.0006BW (R2 = 0.98; P < 0.001). A higher energy-to-protein ratio resulted in higher body fat mass at the same BW (P < 0.001). Sexual maturation was related to body protein mass at 21 wk of age, where each 100 g of body protein mass extra advanced sexual maturation by 5.4 d (R2 = 0.83). Estimates of energetic efficiency for growth (kg) and egg production (ke) appeared not constant, but varied with age in a quadratic manner between 0.27 and 0.54 for kg and between 0.28 and 0.56 for ke. The quadratic relationship could be expressed as kg=0.408-0.0319*Age+0.00181*Age2 (R2 = 0.72; P < 0.001) and ke=-0.211+0.034*Age-0.00042*Age2 (R2 = 0.46; P < 0.001). Body protein mass in broiler breeders is tightly regulated and mainly depended on BW and seems to be the main determinant for sexual maturation. Body fat mass is exponentially related to BW, where an increase in dietary energy-to-protein ratio results in a higher body fat mass. Treatments had minimal effects on estimated energetic efficiencies in breeders.

Two experiments (Exp.) were conducted to evaluate the effects of exogenous carbohydrases on nutrient and energy utilization of corn with different compositions by broilers. In Exp. 1, a total of 448 Cobb 500 male chicks were distributed in a 2 × 4 factorial arrangement (corn from regions geographically located in the North or South of Brazil × 4 carbohydrases supplementation), with 8 replicate cages of 7 birds each. In Exp. 2, 672 Cobb 500 male chicks were fed 12 experimental feeds, in a 3 × 4 factorial arrangement [3 corn endosperm compositions (waxy, semi-dent, or semi-flint) × 4 carbohydrases], with 8 replicate cages of 7 birds. Birds were fed semi-purified test diets with 95.9% corn from d 14 to 24 in both studies. In Exp. 1, α-amylase, β-xylanase, or carbohydrase complex (cellulase, glucanase, and xylanase) were added to the diet. In Exp. 2, α-amylase, β-xylanase, or α-amylase + β-xylanase were supplemented. Digestibility of DM, N, ether extract (EE), Ca, and P as well as AME, AMEn, and IDE were determined. In Exp. 2, jejunal starch digestibility was determined on d 24. Data were subjected to analysis of variance and means were compared by Tukey test (P ≤ 0.05). Corn from the North origin had higher AME, AMEn, and digestibility of DM and N compared to the South (P ≤ 0.05). Amylase supplementation led to increases of 3% in AME and 2% in N digestibility when compared to the non-supplemented feeds (P ≤ 0.01). In Exp. 2, the highest ME values and EE digestibility were observed in the semi-flint corn compared to the waxy, whereas the semi-dent presented the highest digestibility of N and starch. Corn diets supplemented with amylase + xylanase had improvements of 2.5% AMEn and 3% starch digestibility. In conclusion, energy and nutrient utilization of corn by broilers depend on the region where it was grown. Corn genetics, expressed by the endosperm composition, and carbohydrase supplementation influenced energy and nutrient utilization by broilers.

Hyperpigmentation is a skin condition where patches of skin become darker in color due to excess melanin production upon UV exposure leading to melasma, which are lentigines or post inflammatory hyperpigmentation that psychologically affecting a great number of people. The present study investigates the anti-melanogenic effect of Butyroside D and the underling mechanism. After the confirmation of the non-cytotoxic effect of Butyroside D on B16F10 cells, we proceeded with analyzing the impact of the treatment at low and high concentration (i.e., 0.2 μM and 2 μM) using gene profiling analysis and examined the differentiation in gene expression. Our results identify cyclic adenosine monophosphate (cAMP), Wnt/β-catenin and Mitogen-Activated Protein Kinase (MAPK) signaling pathways to be downregulated upon treatment with Butyroside D. These pathways were targeted to further validate the effect of Butyroside D on membrane receptors melanocortin 1 receptor (MC1R) and receptor tyrosine kinase (c-Kit), related microphthalmia-associated transcription factor (MITF) and consequently tyrosinase (TYR), and tyrosine-related protein-1 (TYRP-1) that were all shown to be downregulated and, therefore, leading to the repression of melanin biosynthesis. Finally, the anti-melanogenic effect of Butyroside D was confirmed on human epidermal melanocytes (HEM) cells by inhibiting the activation of cAMP pathway generally mediated through α-melanocyte-stimulating hormone (α-MSH) and MC1R. Overall, this study suggests the potential applicability of this purified compound for the prevention of hyperpigmentation conditions.

This study was performed to investigate the effects of apparent metabolizable energy (AMEn) and protein levels on laying performance, egg quality, blood parameters, blood biochemistry, and apparent total tract digestibility of energy and nutrients in diets fed to laying hens in an aviary system. A total of 560 Hy-Line Brown laying hens (age = 30 week) were distributed in a completely randomized experimental design in 2 × 2 factorial arrangements with 2 metabolizable energy levels (2700 and 2800 kcal AMEn/kg) and 2 protein levels (16.5 and 14.5% CP). Four treatments and four replicates of 40 birds each (stocking density = 15 birds/m2) were prepared. Results revealed no significant interaction between AMEn and CP in the diet in terms of egg production, floor eggs, broken and dirty egg production, egg mass, feed intake, and feed conversion ratio of laying hens. However, egg weight was affected. As dietary energy and CP levels (2800 kcal of AMEn/kg and 16.5% CP) increased, egg weight increased (p < 0.05). Egg weight, feed intake, and feed conversion ratio significantly differed (p < 0.05) as the energy content in the feed increased. Ether extract significantly varied (p < 0.05) as the energy content in the feed increased. In conclusion, laying performance and egg quality of Hy-Line Brown laying hens in the middle stage of egg production (30 to 50 weeks) were not affected by different dietary energy and protein levels, but feed intake decreased with an increasing level of AMEn in diets. Ether extract significantly varied as the energy content in the feed increased.

The chemical composition, antioxidant activity, tannic acid content, mineral, fatty acid, and amino acid profiles of oil-extracted Moringa peregrina seed meal (OEMPSM) were determined. Apparent (AME) and true (AMEn) metabolizable energy and apparent (AAAU) and true (TAAU) amino acid utilization were evaluated using a precision feeding trial. The protein (CP) quality was evaluated by a total efficiency analysis method. The antioxidant activity, gauged by 2,2-diphenyl-1-picrylhydrazyl (DPPH), was 237, 353, and 15.2 mg/mL for the water and ethanol extracts, and ascorbic acid, respectively. Tannic acids were 131.4 mg/100 g dry weight. The OEMPSM had 27.2% CP and 22.4, 15.1, and 15.8 MJ/kg of gross energy, AME and AMEn, respectively. The neutral detergent fiber, acid detergent fiber, and hemicellulose were 40.2, 29.7, and 10.5% DM, respectively. The 15.41% of total fatty acids were saturated and 84.57% unsaturated. The AAAU and TAAU of OEMPSM were 30.92% and 61.06%, respectively. From findings, OEMPSM comprises a valuable level of bioactive substances, amino acids, fatty acids, minerals, and energy; it can provide up to 1.12% of the requirements of total amino acids of chickens (1-21 days); however, the quality of its protein was found to be 44.6% less than that of protein of soybean meal.

We collected stingless bee propolis Tetragonula biroi in order to find materials for medicine and cosmetics applications from tropical rainforest resources. Even though this bee has some biological functions including a cancer cell line, hair growth promotion, asthma remedy, α-glucosidase enzyme inhibition, and antiviral action, the investigation on anti-acne has not been reported yet. This study was to focus on propolis Tetragonula biroi extracts and leads us to isolate active compounds for antioxidant, anti-inflammatory, and anti-acne. We used methanol to obtain the extract from this propolis and assayed it with antioxidants, anti-inflammation, and anti-acne. The extract showed strong activity in antioxidants by DPPH radical scavenging activity (82.31% in 6.25 μg/ml). Via a column chromatography and Reveleris PREP purification system, we isolated 3'-O-methyldiplacone, nymphaeol A, and 5,7,3',4'-tetrahydroxy-6-geranyl flavonol. These compounds showed potential biological activity with IC50 for antioxidant 6.33, 15.49, 17.32 μM; and antiinflammatory 121.54, 121.20, 117.31 μM. The isolated compounds showed anti-acne properties with properties 0.00, 14.11, and 13.78 mm for the inhibition zone (at a concentration of 1 μg/well), respectively. The results indicated that the propolis extract of Tetragonula biroi has the potential to be developed as a cosmetic agent; however, further work needs to be done to clarify its application.

Interleukin 1 (IL-1) has been indicated as a mediator of recurrent pericarditis. Rilonacept, a soluble IL-1 receptor chimeric fusion protein neutralizing interleukin 1 alpha (IL-1α) and interleukin 1 beta (IL-1β), has demonstrated promising results in a phase II study in recurrent or refractory pericarditis. Anakinra is a recombinant inhibitor of the IL-1 receptor with a demonstrated reduction in the incidence of recurrent pericarditis. Definite pharmacological management of pericarditis is key to preventing recurrences, mostly treatment options for recurrent pericarditis refractory to conventional drugs. Here we critically discuss the existing therapy options for recurrent pericarditis, with a focus on new pharmacological approaches: rilonacept and anakinra. A systematic search was conducted across online databases such as PubMed, Cochrane, Google Scholar, ScienceDirect, CINAHL, Scopus, and Embase to obtain clinical trials that assess the effectiveness of anti-interleukin 1 therapy such as anakinra and rilonacept in the management of recurrent pericarditis. Our study concluded that anti-interleukin 1 therapy significantly improved both the quality of life and the clinical outcomes of the study population. These outcomes were most prominent with the use of rilonacept and anakinra in the trial treatment. Rilonacept and anakinra are valuable options in case of recurrent pericarditis refractory to conventional drugs.

Abamectin (ABM) is a common agricultural pesticide and veterinary anthelmintic drug. It can discharge from the sites of application to aquatic systems via surface run-off or spray drift, causing harmful effects to aquatic organisms. The present study investigated the protective effect of dietary quercetin supplementation on hemato-biochemical parameters and hepato-renal oxidative stress biomarkers in Nile tilapia (Oreochromis niloticus) exposed to a sublethal dose of ABM. Fish were allocated into six equal groups. The first group was kept as a control group. The second and third groups (Q400, and Q800) were fed diets supplemented with two quercetin levels (400 and 800 mg/kg diet), respectively. The fourth group (ABM) was intoxicated with 20.73 µg/L of ABM. The fifth and sixth groups (ABM + Q400, and ABM + Q800) were fed diet supplemented with two quercetin levels (400 and 800 mg/kg diet) and simultaneously intoxicated with ABM for 60 days. The results showed that ABM significantly decreased RBCs, hemoglobin content, hematocrit, total protein, albumin levels, and acetylcholinesterase activity activities compared to the control. Meanwhile, ABM significantly increased white blood cells, glucose, total lipids, cholesterol, and alanine and aspartate aminotransferase activities. Liver and kidney levels of lipid peroxidation was significantly increased, while hepato-renal antioxidant biomarkers (reduced glutathione, super oxide dismutase, catalase, and total antioxidant capacity) were significantly decreased upon ABM exposure. On the other hand, quercetin dietary supplementation improved the hemato-biochemical alterations and alleviated oxidative stress induced by ABM exposure. Fish supplemented with quercetin at a level of 800 mg/kg diet showed better alleviating effects against ABM compared to 400 mg/kg diet. Based on these study findings, we suggest that quercetin dietary supplementation (800 mg/kg) offered direct protection against ABM-induced physiological disturbance and oxidative stress in Nile tilapia.

The broader objective of this study is to identify natural materials that might inhibit the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We have focused on stingless bee honey, which has a unique taste that is both sweet and sour and sometimes bitter. We screened 12 samples of honey from 11 species of stingless bees using an angiotensin-converting enzyme 2 (ACE2)-spike protein-binding assay and phytochemical analysis. Ten of the samples showed inhibition above 50% in this assay system. Most of the honey contained tannins, alkaloids, flavonoids, triterpenoids, carotenoids and carbohydrates. Our findings in this in vitro study showed that honey from stingless bees may have a potent effect against SARS-CoV-2 infection by inhibiting the ACE2-spike protein-binding.

Metal-oxide semiconducting materials are promising for building high-performance field-effect transistor (FET) based biochemical sensors. The existence of well-established top-down scalable manufacturing processes enables the reliable production of cost-effective yet high-performance sensors, two key considerations toward the translation of such devices in real-life applications. Metal-oxide semiconductor FET biochemical sensors are especially well-suited to the development of Point-of-Care testing (PoCT) devices, as illustrated by the rapidly growing body of reports in the field. Yet, metal-oxide semiconductor FET sensors remain confined to date, mainly in academia. Toward accelerating the real-life translation of this exciting technology, we review the current literature and discuss the critical features underpinning the successful development of metal-oxide semiconductor FET-based PoCT devices that meet the stringent performance, manufacturing, and regulatory requirements of PoCT.

Frail older people ageing alone in place need help to perform daily living activities, especially when functional limitations are increasing and formal/informal supports are lacking. This context represents a risk of experiencing health emergencies, in particular falls. It is thus important to understand how seniors manage these potential difficulties and who helps them. The present study aimed to explore these dimensions in Italy, where 120 qualitative interviews were carried out in 2019 within the "Inclusive ageing in place" (IN-AGE) research project, involving frail older people living alone at home. A content analysis was conducted. Results showed that seniors need to manage health emergencies regarding heart and breathing problems but mainly episodes of falls are reported, with consequent fractures and fear of falling again. In several cases, the use of a mobile phone was crucial in order to seek for help, and the first to intervene were children, in addition to some neighbors. Some seniors also referred their ability to call independently the General Practitioner (GP) or the emergency room, in order to not disturb family members. These findings highlight new useful insights for policy makers, regarding health emergencies prevention and management measures to put in place, especially concerning falls, and the support provided by communication technologies.

Physical pellet quality and AMEN concentration are strongly related to each other in broiler feeding. A study was conducted to evaluate the relationship between dietary AMEN concentration and feed processing on pellet quality, nutrient digestibility, broiler performance, serum markers, and yield of commercial cuts. Six diets were formulated. The first diet had the recommended AMEN concentration, each further diet was calculated with 40 kcal/kg less, from 0 to -200 kcal/kg, resulting in six levels for each feed phase: starter (1-14 d), grower (15-28 d), and finisher (29-35 d). These diets were processed with and without expander conditioning prior to pelleting, using an average corn particle size of 1.6 mm, ground with a roller mill. A total of 1008 one-day-old male Ross 308 broiler chickens were placed in a 6 × 2 (6 energy levels and 2 conditionings) factorial trial with six boxes as replications, with three in each broiler performance trial period. Excreta were collected 2 days before the end of each feed phase for apparent total tract digestibility measurement. On day 36, four broilers from each replication (pen) were weighed and then euthanized for blood collection, following which the gastrointestinal organs were weighed, and the ileal and gizzard contents were collected. On day 37, all remaining broilers were slaughtered after fasting to measure commercial cuts and abdominal fat. The results show that the pellet durability index (PDI) was most affected by energy reducing and expander conditioning prior to pelleting, and it was better when diets had energy reduced by 40 to 200 kcal/kg (p > 0.001), as when expander conditioning was used. Digestibility of nutrients was slightly affected by treatments, as was the broiler performance; however, feed efficiency was improved in broiler-fed diets without AMEN reduction and when an expander was used, with p = 0.050 and p = 0.031, respectively. No effects were observed on the weight of gastrointestinal tract organs and serum markers, except for liver (p = 0.037) and α-amylase (p = 0.047). The lowest liver weight and lowest serum protein, cholesterol, triglyceride, gamma-glutamyl, and lipase concentrations were obtained when diets were formulated without energy reduction (Ross-0). There was no effect on commercial cuts relative to live weight at slaughter. The energy reduction was well reflected in the proportion of abdominal fat, which decreased when AMEN was reduced (p = 0.001). The present study shows it is possible to use diets with up to 200 kcal/kg reduction in AMEN without losses in performance, and the use of expander conditioning prior to pelleting promotes higher pellet quality and broiler feed efficiency.

A study was conducted to assess the chemical composition and apparent metabolizable energy (AME), nitrogen-corrected AME (AMEn), and their respective coefficients of dry brewery residue (DBR) for broilers. A second study was conducted to evaluate the increasing inclusions of DBR in broiler diets and its effects on growth performance, carcass traits, blood metabolites, and meat quality. In the first trial (metabolism assay), a total of 250 male broilers were assigned to 5 diets (0, 100, 200, 300, and 400 g DBR/kg) composed by 5 replications with 10 animals per cage in a completely randomized design in the period from days 11 to 21. There was an increasing linear effect for AME, AMEn, metabolizable coefficient of gross energy (MCGE), and MCGE corrected for N (MCGEn). In the second experiment, a total of 840 one-day-old male broiler chickens were assigned to 6 treatments (0, 20, 40, 60, 80, and 100 g DBR/kg) with 7 replications and 20 animals per pen, from days 0 to 42. Growth performance was determined at days 21 and 42. There was an increasing linear effect for weight gain in the period from days 0 to 21 and for feed intake from days 0 to 42. A linear decreasing effect was observed at day 21 for cholesterol and quadratic effect for uric acid (UA), aspartate aminotransferase, and albumin. At day 42, there was a quadratic effect for UA and creatinine. At day 21, an increasing linear effect was observed for protein deposition rate. In conclusion, the inclusion of DBR up to 100 g/kg improved WG in the starter phase and did not negatively affect the productive parameters of broilers from days 0 to 42.

The aim of this study was to determine the effect of emulsifier and multicarbohydrase enzyme supplementation on performance, nutrient utilization, and apparent metabolizable energy-nitrogen (AMEN) value of broiler diets containing rapeseed meal (RSM) as well as their influence on the gut morphological structures, excretion of total and free sialic acid, and cecum concentration of short-chain fatty acids (SCFAs) in broiler chickens. A total of 384 male broiler chicks were assigned to four dietary treatments. The diet of the control treatment (CON) consisted of soybean, maize, and RSM (5% in starter, 7% in grower, 15% in finisher) with soybean and palm oils. The diets used for the experimental treatments were the control diet supplemented with an emulsifier (EMU), enzyme (ENZ), or both (EMU + ENZ). The duodenum (n = 10/treatment) and ileum (n = 10/treatment) digesta samples were assessed to determine nutrient digestibility: crude protein (CP), ether extract (EE), starch, Ca. Throughout the experimental period, EMU + ENZ treatment indicated the lowest total average feed intake and feed conversion ratio, with the highest average weight gain among the studied treatments (P < 0.05). The EMU + ENZ treatment also resulted in higher (P < 0.05): apparent prececal digestibility (APD) of CP, total tract neutral detergent fibre (NDF) degradation, apparent total tract digestibility (ATTD) of EE, villus height to crypt depth ratio (P < 0.1). The highest APD of EE was noted in the EMU treatment (P < 0.05). No significant differences were found in the AMEN values of the diets. A greater jejunum villi surface area was found in groups supplemented by enzyme compared to CON (P < 0.05). The EMU + ENZ treatment presented lower sialic acid excretion in the ileum and concentration of cecum SCFAs compared to the CON treatment (P < 0.05). The obtained results indicate that simultaneous usage of additives had beneficial effect on production parameters, nutrient digestibility, NDF degradation, as well as gut mucosa morphology. Based on the SCFAs concentration results, separate or simultaneous addition of emulsifier or/and enzyme did not provoke excessive fermentation activity of cecal bacteria.

A total of 180 female Japanese quail breeders were allocated to 5 treatments with 6 replicates of 6 birds and fed a diet supplemented with 0, 125, 250, 500, or 1000 mg/kg of a phytogenic feed additive (PFA) in a 9-wk experiment. Egg weight, feed efficiency, shell breaking strength and calcium content, specific gravity, Haugh unit, and percentages of fertile eggs increased with increasing PFA levels (P < 0.05). Increasing of PFA levels increased nutrient digestibility, dietary AMEn, and activities of digestive enzymes in the pancreas and intestinal digesta (P < 0.05). Supplementation of PFA reduced Escherichia coli (P < 0.05), Clostridium spp. (P < 0.05) and Salmonella spp. counts (P < 0.05), while increased Lactobacillus and Bifidobacterium spp. counts in the ileal and cecal contents (P < 0.05). Dietary PFA increased jejunal villus height and decreased ileal crypt depth (P < 0.05). Serum diamine oxidase activity and D-lactate level were decreased with increase in PFA level (P < 0.05). Increasing PFA levels increased glutathione peroxidase activity in the pancreas, small intestine, and cecal tonsil, but decreased malondialdehyde contents (P < 0.05). Birds fed PFA exhibited increased levels of secretory IgA in the intestinal mucosa (P < 0.05), and increased the percentage of CD3+ T cells, ratio of CD4+/CD8+ T cells, and cytokine concentrations in the cecal tonsils (P < 0.05). In conclusion, PFA could improve gut health and nutrient utilization and, therefore, benefit productivity, egg quality, and fertility in quails.

The objective of this experiment was to determine the energy values and nutrient utilisation of five corns at starer (14-16 days) and grower (28-30 days) phases. The 1-5 corn samples were obtained from Jinzhou, Liaoning; Gongzhuling, Jilin; Qingdao, Shandong; Songyuan, Jilin and Xinxiang, Henan, China. Each phase of the trial consisted of six treatments with six replicates each. The number of birds per replicate was 4 (Days 14-16) and 2 (Days 28-30). Diets included a corn-soybean meal basal diet and five test diets contained 30% of each corn sample, which replaced 30% of the energy-yielding components of the basal diet (as-fed basis). The trial consisted of six cycles, each cycle was 7 days, including 4 days for adaptation to feed. On Day 4, the birds were transferred to the respiration chambers. In the following 3 days, total excreta were collected and daily heat production was measured. The apparent total tract retention of dry matter, crude protein, ether extract, and starch improved with age, showing the same trend as energy values. The nitrogen-corrected metabolisable energy (AMEn) values for corn samples at the starter phase were in the range of 16.25-17.41 MJ/kg DM and at the grower phase were in the range of 17.23-17.60 MJ/kg DM. On the other hand, the net energy (NE) values of the five corn samples ranged from 9.93 to 11.67 MJ/kg DM during the starter period and from 11.19 to 12.27 MJ/kg DM during the grower period. The AMEn and NE values of the five corn samples were close to the literature values for corn. Results from our research indicated the nutrient digestibility and energy values of corn samples increase with the age of the broiler.

In the available literature, there are limited data about the energetic value of insect-derived products. In particular, insect fat cannot be used in practical broiler nutrition due to the lack of precise apparent metabolisable energy (AME) value. Therefore, the present study aimed to investigate the AME and apparent metabolisable energy corrected to zero nitrogen balance (AMEN) levels of Hermetia illucens larvae fat for broiler chickens of various ages. A total of 400 1-day-old male Ross 308 chicks were randomly allotted to four dietary groups (10 replicate pens per treatment; 10 birds per pen). The following treatments were applied: HI0 - basal diet without dietary fat inclusion, HI03 - basal diet enriched with 30 g/kg H. illucens larvae fat, HI06 - basal diet enriched with 60 g/kg H. illucens larvae fat, and HI09 - basal diet enriched with 90 g/kg H. illucens larvae fat. Broilers had ad libitum access to mash form feed and water. Excreta samples were collected on d 14, d 28, and d 35. To determine the AME and AMEN values of H. illucens larvae fat, the simple linear regression method was used. The results show that the AME and AMEN values of H. illucens larvae fat for broiler chickens are 9 049 kcal/kg (37.86 MJ/kg) and 9 019 kcal/kg (37.74 MJ/kg), respectively. Additionally, because the birds' age significantly (P < 0.001) affected the AME and AMEN levels, the implementation of H. illucens larvae fat to broiler diets should be considered in each nutritional period using the recommended regression model AME = 2 559.758 + 62.989 × fat inclusion (%) + 7.405 × day of age and AMEN = 2 543.2663 + 62.8649 × fat inclusion (%) + 7.3777 × day of age. The present data emphasised that the H. illucens larvae fat metabolisable energy is similar to that of soybean oil.

The influence of the energy and the standardized ileal digestible lysine (DLys) content of the diet on egg production and egg quality, was studied in brown-egg laying hens from 18 to 41 wk of age. The experimental design was completely randomized with 10 treatments organized as a 2 × 5 factorial with 2 energy concentrations (2,750 and 2,800 kcal AMEn/kg) and 5 levels of DLys (values varied from 0.66 to 0.78% and 0.67 to 0.79%, for the low and high energy diets, respectively). Each treatment was replicated 10 times (10 hens per replicate). The data were analyzed using the MIXED procedure of SAS with energy concentration and DLys content of the diets as main effects. In addition, the effects of the DLys on the variables studied were partitioned into its lineal and quadratic components. From 18 to 21 wk of age (pre-peak phase), diet composition had limited effects on egg production. From 22 to 41 wk of age (peak phase), however, an increase of 50 kcal AMEn/kg diet increased egg weight (P < 0.05) and tended to improve energy intake (P = 0.083) and feed conversion ratio (P = 0.074). An increase in DLys improved linearly (P < 0.001) egg production, egg weight, egg mass, feed conversion, and energy conversion ratio, and tended to increase BW gain (P = 0.074). Diet composition did not any affect egg quality trait except shell strength that increased linearly (P < 0.05) with increases in the DLys. Cumulatively (18-41 wk of age), egg weight increased (P < 0.05) as the energy and the DLys content of the diet increased. In summary, an increase in energy and DLys content of the diet had limited effects on egg production during the prepeak phase but improved egg production, feed conversion ratio, and BW gain during the peak phase. The data indicate that hens require at least 839 mg DLys/d to maximize egg production in the peak production phase.

During the processing of compound feed for broilers, several changes occur that affect the physical and probably the nutritional properties of pellets, influencing animal performance. The effects of mill type, particle size (PS) and expander conditioning prior to pelleting (E + P) were combined to generate pellets. A 2 × 3 × 2 factorial arrangement was designed with two mill types (a hammer mill (HM) or roller mill (RM)), three PSs (0.8, 1.2 or 1.6 mm) and two E + Ps (with or without expander processing prior to pelleting), with six replications of 12 unsexed Ross 308 broilers each. All the processing lines reduced the PS from mash to finished pellets via secondary grinding, by 2.35 times on average. However, RM grinding required less electric power (p < 0.001). The intended PS (0.8, 1.2 or 1.6 mm) did not affect this energy consumption. E + P and the PS interacted for the pellet durability index (PDI) (p = 0.006). The worst PDI in the pellets was observed when a PS of 1.6 mm without E + P was used. Only E + P positively affected starch (p < 0.001) and amino acids' ileal apparent digestibility (p < 0.01). Organic matter (OM) (p = 0.02) and fat (p < 0.001) digestibility, as well as AMEN (p = 0.005) content, were influenced by the PS (main effect), whereas E + P and mill type interacted with these values (p < 0.005). Lower OM digestibility and AMEN content were observed when RM without E + P was used (p = 0.001). The feed conversion ratio (FCR) was enhanced and feed intake (FI) was improved with E + P. The combination of the RM mill, a 1.6 mm mean PS, and E + P improved FCR (three-way interaction, p = 0.019)), showing that for a higher PS, E + P is necessary for animal performance. Carcass yield was, on average, 80.1%. No effects on commercial cuts (breast, legs and wings) were observed. In contrast, abdominal fat was affected by mill type * PS (p = 0.012) and E + P * PS (p = 0.048) in a two-way interaction. The highest abdominal fat indicated an imbalance in the amino acid (AA)-to-AMEN ratio. Coarse PS promoted heavier gizzards (p = 0.02) but E + P tended to reduce them (p = 0.057). The processing steps improved pellet quality and feed efficiency associated with RM, coarse PS and E + P, highlighting the positive effects of E + P on abdominal fat and AMEN content, which should be adjusted to AA or reduced at formulation. However, these results are for an experimental processing plant and may not necessarily apply to larger plants, so the use of these data and methods should be considered as guidelines for replication at production sites.

Organic pollutants (OPs) including organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and polycyclic aromatic hydrocarbons (PAHs) have showed neuro-damaging effects, but studies concerning the autism spectrum disorder (ASD) risk are limited. A case-control study with ASD (n = 125) and healthy control (n = 125) children was conducted on the different land use settings across Punjab, Pakistan. Serum concentrations of 26 OCPs, 29 PCB congeners, 11 PBDEs and 32 PAHs were measured. Serum PCB77 (AOR = 2.00; 95% CI: 1.43, 2.18), PCB118 (AOR = 1.49; 95% CI: 1.00, 2.00), PCB128 (AOR = 1.65; 95% CI: 1.01, 1.91), PCB153 (AOR = 1.80; 95% CI: 1.55, 1.93) were significantly higher, but PCB187 (AOR = 0.37; 95% CI: 0.24, 0.49) was significantly lower in the ASD cases when compared to the controls. Serum BDE99 (AOR = 0.48; 95% CI: 0.26, 0.89) was significantly higher in the healthy controls than in the ASD cases. Among the analyzed OCPs, p,p'-DDE (AOR = 1.50; 95% CI: 1.00, 1.85) was significantly elevated in the ASD cases with comparison in the controls. For PAHs, serum dibenzothiophene (AOR = 7.30; 95% CI: 1.49, 35.85) was significantly higher in the ASD, while perylene (AOR = 0.25; 95% CI: 0.06, 1.10) and fluorene (AOR = 0.21; 95% CI: 0.06, 0.72) were significantly higher in the controls. In addition, many of the serum pollutants were significantly associated with GSTT1, GSTM1 (null/present polymorphism) and presented the genotypic variation to respond xenobiotics in children. The children living in proximity to urban and industrial areas had a greater exposure to most of the studied pollutants when compared to the rural children, however children residing in rural areas showed higher exposure to OCPs. This comprehensive study documents an association between environmental exposure risk of several organic pollutants (OPs) from some contaminated environmental settings with ASD risk in children from Pakistan.

The purpose of this scoping review is to investigate the value of creative arts therapies in healthy older adults. This article aims to shed light on current knowledge concerning the effectiveness of art therapies (ATs) for the prevention of common age-related conditions using the definition of art therapy provided by the American Art Therapy Association (AATA), as well as Cohen's conceptual framework for the psychological conceptualization of the relationship between the arts and health in later life. The objective is to carefully capture subthreshold situations of distress, which are often not taken into account and primarily involve psychological aspects that are crucial in the multidimensional perspective of healthy aging. Twelve articles were selected and examined following an initial electronic search on 3 databases. A thematic analysis of the results identified four major themes: improving cognitive performance and proprioception; enhancing self-identity and meaningful life; reducing feelings of loneliness and depressive symptoms; and the importance of socialization. All these aspects constitute the basis for preventing psychological distress and enhancing mental well-being for healthy aging.