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2,023 results
  • A carbonic anhydrase pseudogene sensitizes select Brucella lineages to low CO2 tension. [Journal Article]
    J Bacteriol 2019Varesio LM, Willett JW, … Crosson S
  • Brucella are intracellular pathogens that cause a disease known as brucellosis. Though the genus is highly monomorphic at the genetic level, species have animal host preferences and some defining physiologic characteristics. Of note is the requirement for CO2 supplementation to cultivate particular species, which confounded early efforts to isolate B. abortus from diseased cattle. Differences in …
  • BCAT1 and BCAT2 disruption in CHO cells has cell line-dependent effects. [Journal Article]
    J Biotechnol 2019Pereira S, Ley D, … Rørdam Andersen M
  • In recombinant protein expression using Chinese hamster ovary (CHO) cells, chemically defined media contain essential amino acids such as branched chain amino acids (BCAAs) leucine, isoleucine and valine. Availability of amino acids is critical as these are building blocks for protein synthesis. However, breakdown of amino acids can lead to build up of toxic intermediates and metabolites that dec…
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