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2,098 results
  • An Inexpensive Staining Alternative for Gelatin Zymography Gels. [Journal Article]
    Methods Protoc 2019; 2(3)Wechselberger C, Doppler C, Bernhard D
  • Zymography is a widely used electrophoretic method to determine proteolytic activities in samples from various sources. The method is based on copolymerizing a suitable protein substrate within a sodium dodecyl sulfate-polyacrylamide gel. Following electrophoretic separation of the protease containing samples and a suitable incubation period, degradation of the substrate can be visualized through…
  • In vitro reconstitution of yeast RNA polymerase II transcription initiation with high efficiency. [Journal Article]
    Methods 2019; 159-160:82-89Fujiwara R, Murakami K
  • Transcription initiation can be reconstituted from highly purified general transcription factors (GTFs), RNA polymerase II (pol II), and promoter DNA. However, earlier biochemical reconstitution systems had a serious technical limitation, namely very poor initiation efficiency. Due to the poor efficiency of the reaction and trace amounts of proteins involved in the pre-initiation complex (PIC) as…
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