This study describes the validation of a approach to detect in herbal supplements regulated Hoodia species or their extracts, targeting the oxypregnane steroidal glycoside P57AS3, using liquid chromatography coupled to tandem mass spectrometry with a high-resolution acute mass detection (LC-HRMS/MS). The method was validated by assessing the selectivity and specificity by analysing other related Apocynaceae species, particularly Stapeliinae subtribe members, and various plants commonly found in dietary supplements promoting weight loss. The limit of detection (LOD), equal to 8 µg P57AS3 per gram powder, matrix effects, and the ratio of false positives (0%) were determined. Next, the analytical sensitivity of the LC-HRMS/MS methodology was compared to a recently developed quantitative PCR (qPCR) method by comparing the results obtained from 20 commercial herbal supplements, often originating from rogue internet pharmacies, that either claimed or were reported to contain Hoodia sp. material or extracts thereof. The study results highlight the complementary nature of LC-HRMS/MS and qPCR. While qPCR provides high molecular sensitivity for powdered material, LC-HRMS/MS delivers chemical confirmation and detection of metabolites when DNA is absent or degraded. The integration of both techniques creates a comprehensive detection framework, enhancing accuracy, reliability, and confidence in the identification of regulated plant material across different and complex herbal matrices and mixtures thereof.

Plants belonging to the Hoodia genus are widely used as ingredients in herbal dietary supplements. Rising demand and overharvesting have led to supply shortages, adulteration, and CITES-regulated trade, threatening the authenticity and safety of Hoodia in commercial products. These factors highlight the need for a reliable genus-level detection method for Hoodia to support the control of illegal trade and the verification of marketed Hoodia-based products. To address this need, we developed a real-time PCR method specific to Hoodia genus targeting the ITS2 region, a molecular marker with high variability enabling detection and differentiation of plant species or genera. Due to the limited number of publicly available Hoodia ITS2 sequences, 13 additional sequences were generated in-house to support assay design. This real-time PCR method was validated in compliance with international standards, demonstrating high specificity, high sensitivity, and inter-laboratory transferability. Its applicability was verified by testing commercial herbal dietary supplements. This study provides enforcement laboratories with a validated molecular tool to support Hoodia monitoring and authentication in commercial products.

The popularity of plant-based food supplements has risen all across the globe, supported by the assumption that anything natural is safe. However, these supplements usually consist of various plant mixtures, posing a problem for the detection of undeclared regulated plants. A combination of mass spectrometry (MS) and chemometrics is applied in this paper to provide an overview of the presence of regulated plants in plant food supplements across the illegal and legal markets in Belgium. Regulated plants, i.e. Aristolochia fangchi, Ilex paraguariensis, Garcinia cambogia, and Hoodia gordonii, which are popular for weight loss applications, were evaluated. After generating fingerprints and applying chemometric techniques, the dominating presence was found of two plants (Ilex paraguariensis and Garcinia cambogia) in both the illegal and legal markets. The prohibited plant, Aristolochia fangchi, could also be detected in some samples. This raises a matter of concern and necessitates stricter control regarding plant food supplements entering the Belgian market and sheds light on its importance for consumer's health.

Oxidosqualene cyclases (OSCs) are the key enzymes accountable for the cyclization of 2,3-oxidosqualene to varied triterpenoids and phytosterols. Hoodia gordonii (from the family Apocynaceae), a native of the Kalahari deserts of South Africa, Namibia, and Botswana, is being sold as a prevalent herbal supplement for weight loss. The appetite suppressant properties are attributed to P57AS3, an oxypregnane steroidal glycoside. At the molecular level, the enzymes involved in the biosynthesis of triterpenes and phytosterols from H. gordonii have not been previously reported. In the current study, predicted transcripts potentially encoding oxidosqualene cyclases were recognized first by searching publicly available H. gordonii RNA-seq datasets. Two OSC-like sequences were selected for functional analysis. A monofunctional OSC, designated HgOSC1 which encodes lupeol synthase, and HgOSC2, a multifunctional cycloartenol synthase forming cycloartenol and other products, were observed through recombinant enzyme studies. These studies revealed that distinct OSCs exist for triterpene formation in H. gordonii and provided opportunities for the metabolic engineering of specific precursors in producing phytosterols in this plant species.

Obesity and associated complications including diabetes, cardiometabolic dysfunction, disability, malignancy and premature mortality are considered epidemic. Research on obesity is therefore of worldwide importance. The development of obesity is a multifactorial phenomenon with contributions from biological, behavioral, genetic and environmental factors. Obesity and its associated issues require various lifestyle modifications and treatment options such medication, exercise, diet, surgery, pharmacological therapy and dietary supplements. Dietary supplements are considered an attractive alternative to traditional therapy due to their low toxicity profile and their accessibility to the general population. Dietary supplements may include one or more dietary ingredients. In this narrative review, we analyze the effects on obesity and obesity-related issues of various natural components. For example, there are a myriad of supplements that have been used as dietary supplements for weight loss such as minerals, vitamins, amino acids, metabolites, herbs, and plant extracts. This narrative review aims to present the benefits and side-effects of several ingredients of dietary supplements for weight loss and treatment of obesity. In particular, the mechanism of action, results of clinical trials, and possible side effects will be presented for the following ingredients: β-Glucans, bitter orange, calcium, vitamin D, chitosan, chromium, cocoa, coleus forskohlii, conjugate linoleic acid, ephedra sinica, fucoxanthin, garcinia cambogia, glucomannan, green coffee, green tea, guar gum, raspberry, hoodia gordonii, irvingia gabonensis, phenylpropylamine, pyruvate, white kidney bean.

Due to the increase in the prevalence of obesity, the consumption of dietary supplements promoting weight loss has increased in the last few years. Many dietary supplements are available on the market, such as L-carnitine, glucomanan, chitosan, and Hoodia gordonii. The safety and clinical effectiveness of most of these supplements have not been assessed by high-quality trials. The scientific evidence of their benefit remains weak. The risk of adverse effects and drug interactions, most often unknown to patients, exists and must be carefully considered before initiating supplementation, in consultation with a physician, in the same way as other aspects of the treatment of obese patients.

Single phase CeO2 nanocrystals were bio-synthesized using Hoodia gordonii natural extract as an effective chelating agent. The nanocrystals with an average diameter of 〈Ø〉 ~ 5-26 nm with 4+ electronic valence of Ce displayed a remarkable UV selectivity and an exceptional photostability. The diffuse reflectivity profile of such CeO2 exhibited a unique UV selectivity, in a form of a Heaviside function-like type profile in the solar spectrum. While the UV reflectivity is significantly low; within the range of 0.7%, it reaches 63% in the VIS and NIR. Their relative Reactive Oxygen Species (ROS) production was found to be < 1 within a wide range of concentration (0.5-1000 μg/ml). This exceptional photostability conjugated to a sound UV selectivity opens a potential horizon to a novel family of green nano-cosmetics by green nano-processing.

Metabolic syndrome is recognized as a proinflammatory condition leading to hepatic steatosis and nonalcoholic steatohepatitis (NASH). We tested the effects of a succulent species Hoodia parviflora N.E. Br., of the genus Hoodia sweetex Dence, on animal models of NASH and insulin resistance (ob/ob mouse and the sand rat Psammomys obesus). IL6 secretion was evaluated by ELISA and hepatic signal transducer and activator of transcription 3 by Western blot. We followed liver enzymes, weight, glucose, hepatic histology, hepatic triglycerides (TGs), and total fat and serum insulin. Oral administration of extracts derived from H. parviflora alleviated the insulin resistance manifested by improved glucose tolerance tests. Treatment alleviated the liver injury noted by a decrease in liver enzyme levels, improved intrahepatic TG content, total hepatic fat, and improved hepatic histology. Similarly, treatment with H. parviflora reduced hepatic inflammation in mice with Concanavalin A-induced hepatitis. These effects were independent of food consumption and weight. H. parviflora was associated with alleviated insulin resistance, hepatic steatosis, and liver injury. The data support its use as a liver protector.

Rising rates of obesity across the globe have been associated with an increase in the use of herbal preparations for weight control. However, the mechanisms of action for these substances are often not known, as is the potential for interaction with other herbal preparations or prescription pharmaceutical drugs. To investigate the reported efficacy and safety of herbal weight loss preparations, we conducted a review of the literature focusing on herbs that are most commonly used in weight loss preparations, specifically, Garcinia cambogia, Camellia sinensis, Hoodia gordonii, Citrus aurantium and Coleus forskohlii. There was no clear evidence that the above herbal preparations would cause sustained long-term weight loss in humans in the long term. Serious illness and even death have occasionally resulted from the use of herbal weight loss preparations. Few clinical trials have been undertaken to evaluate the efficacy and/or safety of herbal weight loss preparations. In addition, potential issues of herb-herb and herb-drug interactions are often not considered. Regulation of these products is much less rigorous than for prescription medications, despite documented cases of associated hepatotoxicity.

Hoodia is succulent, cactus-like plant of the Kalahari Desert, extracts of which are marketed as weight loss aids. Hoodia has not been implicated in causing serum enzyme elevations or clinically apparent liver injury.

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on the dried aerial parts of Hoodia parviflora as a novel food (NF) submitted pursuant to Regulation (EC) No 258/97. The information provided on the composition, the specifications, the production process, the batch-to-batch variability and the stability of the NF is sufficient and does not raise safety concerns. The applicant intends to use the NF in a number of energy-reduced/sugar-free/no-added-sugar foods in quantities of up to 15 mg per serving. The applicant also proposes to provide the NF as a food supplement. The target population proposed by the applicant is adults. The highest intake estimates were found in the group of elderly (≥ 65 years) individuals, with a high intake of 1.0 mg/kg body weight (bw) per day. One 90-day toxicity study in rodents was provided from which a benchmark dose lower confidence limit (BMDL 05) of 53.5 mg/kg bw per day was derived for effects of the NF on bodyweight. The Panel concludes that the addition of the NF to foods as a food ingredient at the uses and use levels as proposed by the applicant would exceed intake levels considered safe in humans. The Panel considers that the NF is safe to be used as a food supplement at a maximum dose of 9.4 mg/day. The target population is adults.

Ultrahigh-performance liquid chromatography quadrupole-time-of-flight mass spectrometry (UHPLC-QToF-MS) profiling was used for the identification of marker compounds and generation of metabolic patterns that could be interrogated using chemometric modeling software. UHPLC-QToF-MS was used to generate comprehensive fingerprints of three botanicals (Hoodia, Terminalia, and chamomile), each having different classes of compounds. Detection of a broad range of ions was carried out in full scan mode in both positive and negative modes over the range m/z 100-1700 using high-resolution mass spectrometry. Multivariate statistical analysis was used to extract relevant chemical information from the data to easily differentiate between Terminalia species, chamomile varieties, and quality control of Hoodia products. Using nontargeted analysis, identification of 37 compounds contributed to the differences between Terminalia species, 26 flavonoids were identified to show the differences between German and Roman chamomile, and 43 pregnane glycosides were identified from Hoodia gordonii samples. The UHPLC-QToF-MS-based chemical fingerprinting with principal component analysis was able to correctly distinguish botanicals and their commercial products. This work can be used as a basis to assure the quality of botanicals and commercial products.