- Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins - the introduction of protein-corona-free polymer nanomedicine. [Journal Article]
- NNanoscale 2018 Mar 21
- In this paper, we revised the current understanding of the protein corona that is created on the surface of nanoparticles in blood plasma after an intravenous injection. We have focused on nanopartic...
In this paper, we revised the current understanding of the protein corona that is created on the surface of nanoparticles in blood plasma after an intravenous injection. We have focused on nanoparticles that have a proven therapeutic outcome. These nanoparticles are based on two types of biocompatible amphiphilic copolymers based on N-(2-hydroxypropyl)methacrylamide (HPMA): a block copolymer, poly(ε-caprolactone) (PCL)-b-poly(HPMA), and a statistical HPMA copolymer bearing cholesterol moieties, which have been tested both in vitro and in vivo. We studied the interaction of nanoparticles with blood plasma and selected blood plasma proteins by electron paramagnetic resonance (EPR), isothermal titration calorimetry, dynamic light scattering, and cryo-transmission electron microscopy. The copolymers were labeled with TEMPO radicals at the end of hydrophobic PCL or along the hydrophilic HPMA chains to monitor changes in polymer chain dynamics caused by protein adsorption. By EPR and other methods, we were able to probe specific interactions between nanoparticles and blood proteins, specifically low- and high-density lipoproteins, immunoglobulin G, human serum albumin (HSA), and human plasma. It was found that individual proteins and plasma have very low binding affinity to nanoparticles. We observed no hard corona around HPMA-based nanoparticles; with the exception of HSA the proteins showed no detectable binding to the nanoparticles. Our study confirms that a classical "hard corona-soft corona" paradigm is not valid for all types of nanoparticles and each system has a unique protein corona that is determined by the nature of the NP material.
- Noninvasive magnetic resonance/photoacoustic imaging for photothermal therapy response monitoring. [Journal Article]
- NNanoscale 2018 Mar 21
- In vivo assessment of vascular permeability and therapeutic response provides novel insights into photothermal therapy (PTT) that is currently under clinical investigation. We have developed noninvas...
In vivo assessment of vascular permeability and therapeutic response provides novel insights into photothermal therapy (PTT) that is currently under clinical investigation. We have developed noninvasive imaging strategies to improve the monitoring of nanoparticle-mediated PTT responses for personalized nanomedicine. Briefly, dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and photoacoustic imaging (PAI) were applied to study the enhanced permeability and retention (EPR) effect in tumor models of different microvascular permeabilities (i.e., 4T1 mouse breast tumor model and HUH-7 human hepatoma model in nude mice). Magnetic resonance temperature imaging (MRTI) and diffusion-weighted MRI (DWI) showed that the 4T1 tumor model exhibits a higher PTT temperature response than that of the HUH-7 tumor model. Our findings demonstrate that the combined use of MRI and PAI techniques is useful in monitoring the vascular permeability and temperature status following PTT, promising to help guide PTT in future translational investigation.
- Antitumor effect of a Pt-loaded nanocomposite based on graphene quantum dots combats hypoxia-induced chemoresistance of oral squamous cell carcinoma. [Journal Article]
- IJInt J Nanomedicine 2018; 13:1505-1524
- CONCLUSIONS: Taken together, our results show that GPt demonstrates superiority in combating hypoxia-induced chemoresistance. It might serve as a novel strategy for future microenvironment-targeted cancer therapy.
- The theranostic efficiency of tumor-specific, pH-responsive, peptide-modified, liposome-containing paclitaxel and superparamagnetic iron oxide nanoparticles. [Journal Article]
- IJInt J Nanomedicine 2018; 13:1495-1504
- CONCLUSIONS: Considering all our in vitro and in vivo results, we conclude that we developed targeting modified theranostic liposome which could achieve both role of antitumor and MRI.
- Biodistribution, pharmacokinetics, and toxicity of dendrimer-coated iron oxide nanoparticles in BALB/c mice. [Journal Article]
- IJInt J Nanomedicine 2018; 13:1483-1493
- CONCLUSIONS: Although more systematic studies are still required, our results encouraged the future investigations of G4@IONPs in biomedical applications.
- Targeting experimental orthotopic glioblastoma with chitosan-based superparamagnetic iron oxide nanoparticles (CS-DX-SPIONs). [Journal Article]
- IJInt J Nanomedicine 2018; 13:1471-1482
- CONCLUSIONS: Hybrid chitosan-dextran magnetic particles demonstrated high MR contrast enhancing properties for the delineation of the brain tumor. Due to a significant retention of the particles in the tumor an application of the CS-DX-SPIONs could not only improve the tumor imaging but also could allow a targeted delivery of chemotherapeutic agents.
- Endocytic mechanisms and osteoinductive profile of hydroxyapatite nanoparticles in human umbilical cord Wharton's jelly-derived mesenchymal stem cells. [Journal Article]
- IJInt J Nanomedicine 2018; 13:1457-1470
- CONCLUSIONS: Our data provide additional insight about the interactions of HANPs with MSCs and suggest their application potential in hard tissue regeneration.
- Debugging Nano-Bio Interfaces: Systematic Strategies to Accelerate Clinical Translation of Nanotechnologies. [Review]
- TBTrends Biotechnol 2018 Mar 17
- Despite considerable efforts in the field of nanomedicine that have been made by researchers, funding agencies, entrepreneurs, and the media, fewer nanoparticle (NP) technologies than expected have m...
Despite considerable efforts in the field of nanomedicine that have been made by researchers, funding agencies, entrepreneurs, and the media, fewer nanoparticle (NP) technologies than expected have made it to clinical trials. The wide gap between the efforts and effective clinical translation is, at least in part, due to multiple overlooked factors in both in vitro and in vivo environments, a poor understanding of the nano-bio interface, and misinterpretation of the data collected in vitro, all of which reduce the accuracy of predictions regarding the NPs' fate and safety in humans. To minimize this bench-to-clinic gap, which may accelerate successful clinical translation of NPs, this opinion paper aims to introduce strategies for systematic debugging of nano-bio interfaces in the current literature.
- Polymorphism genotyping based on loop-mediated isothermal amplification and smartphone detection. [Journal Article]
- BBBiosens Bioelectron 2018 Mar 08; 109:177-183
- The genotyping of a single-nucleotide polymorphism (SNP) is addressed through methods based on loop-mediated isothermal amplification (LAMP) combined with user-friendly optical read-outs to cover the...
The genotyping of a single-nucleotide polymorphism (SNP) is addressed through methods based on loop-mediated isothermal amplification (LAMP) combined with user-friendly optical read-outs to cover the current demand for point-of-care DNA biomarker detection. The modification of primer design and reaction composition improved the assay selectivity yielding allele-specific results and reducing false-positive frequency. Furthermore, the reduced cost, ease of use and effectiveness of colorimetric detection (solution and hybridisation chip formats) were availed for the image capture by a smartphone, reching high sensitivity. In order to evaluate their discriminating capacities, LAMP-based methods were applied to human samples to genotype a SNP biomarker (rs1954787) located in the GRIK4 gene and related to the treatment response to anti-depressants drugs. Sensitive (limit of detection: 100 genomic DNA copies), reproducible (< 15% error), fast (around 70 min) and low-cost assays were accomplished. Patient subgroups were correctly discriminated, agreeing with reference sequencing techniques. The achieved analytical performances using the developed amplification-detection principles confirmed the approach potential for point-of-care optical DNA testing.
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- Ocular findings in Zucker Diabetic Fatty rats emphasize the key role of neuroglia degeneration in diabetic retinopathy pathophysiology. [Journal Article]
- NRNeural Regen Res 2018; 13(2):239-240