- Right ventricular total isovolumic time: Reference value study. [Journal Article]
- EEchocardiography 2019 Jun 04
- CONCLUSIONS: The normal values of RV tIVT increase with age and correlate significantly with Doppler diastolic parameters.
- A calibration transfer optimized single kernel near-infrared spectroscopic method. [Journal Article]
- SASpectrochim Acta A Mol Biomol Spectrosc 2019 May 07; 220:117098
- Single kernel near-infrared spectroscopy (SKNIRS) could aid in the quality screening of early-generation seeds, to improve the efficiency of seed breeding. However, the application of SKNIRS is limit…
Single kernel near-infrared spectroscopy (SKNIRS) could aid in the quality screening of early-generation seeds, to improve the efficiency of seed breeding. However, the application of SKNIRS is limited due to the irregular physical characteristics, the heterogeneous constituent distributions of individual seeds, and the insufficient detection accuracy of the reference method. The reported near-infrared detection results of single seeds are often less accurate than those of dehusked seeds and seed flour. In this paper, a calibration transfer-optimized single kernel near-infrared spectroscopic method is proposed. This method aims to accurately detect the chemical composition of single seeds by using the calibration model of the corresponding dehusked seeds or seed flour. The proposed method was applied to the analysis of the protein content of a single rice kernel. The near-infrared transmission spectra of three forms of rice (single rice kernel (SRK), single brown rice kernel (SBK) and rice flour (RF)) of 201 individual rice seeds and the corresponding protein content values were obtained. By comparing different pretreatment methods and spectral ranges, the spectral range 950-1250 nm, the standard normal variate transformation (SNV) pretreatment, and 9 PLS factors were selected to construct the optimal partial least squares (PLS) regression models. Then, the protein content of single rice kernels were determined through two different methods: (i) the direct method, in which single rice kernels were analyzed using the single rice kernel model directly; and (ii) the proposed method, in which the spectra of single rice kernels were transferred into the spectra of single brown rice kernels and rice flours with a calibration transfer algorithm, spectral space transformation (SST), and were analyzed by the respective calibration models. The external validation coefficient correlation (R) value of the direct method was 0.971, and the R values of the proposed method were 0.962 (SBK) and 0.975 (RF). The root mean square error of prediction (RMSEP) value of the direct method was 0.423, and the RMSEP of the proposed method were 0.480 (SBK) and 0.401 (RF). In addition, the transfer results among the spectra of three forms of rice were compared. By comparison, the results of the proposed method are fairly close to the results of the direct method. The results indicate that the spectra generated from one individual rice seed can be transferred freely among the three forms by means of calibration transfer. The proposed method is a promising way to overcome the challenges associated with analyzing individual seeds and to improve SKNIRS.
- REFERENCE INTERVALS FOR PLASMA BIOCHEMISTRY OF HEMOLYMPH IN SUBADULT CHILEAN ROSE TARANTULA (GRAMMASTOLA ROSEA) UNDER CHEMICAL RESTRAINT. [Journal Article]
- JZJ Zoo Wildl Med 2019; 50(1):127-136
- Tarantulas are a commonly kept species that are occasionally presented to veterinarians in exotic practice. A recent study on Grammastola rosea hemolymph biochemistry has been performed with nonanest…
Tarantulas are a commonly kept species that are occasionally presented to veterinarians in exotic practice. A recent study on Grammastola rosea hemolymph biochemistry has been performed with nonanesthetized adult theraphosids. The objective of this study was to produce reference intervals for biochemistry biomarkers in hemolymph of chemically restrained G. rosea for use diagnostically by exotic veterinarians. Cardiac hemolymph collection was performed on 20 subadult tarantulas under general anesthesia with isoflurane. Samples were processed by a commercial laboratory. Statistics performed on the data include outlier exclusion, descriptive statistics, normality tests, and Pearson correlations. Reference intervals were made for total protein, creatine kinase (CK), aspartate aminotransferase (AST), glucose, uric acid (UA), calcium, and phosphorus. No cortisol was detected. The majority of the intervals produced were normally distributed with the exceptions of UA, phosphorus, and CK. Pearson correlation tests found several significant (P = <0.05) correlations between variables. The majority of the data displayed a normal distribution, unlike the previous study, with a greater number of replicates. The total protein, glucose, UA, calcium, and AST values generated were similar to those reported in the previous study. Conversely several variables such as phosphorus, CK, and albumin were not consistent with those previously reported. Evidence is presented for a lack of albumin, CK, and AST in Arachnida and thus previous data for these proteins is likely to be artifactual.
- Simultaneous quantification of 48 plasma amino acids by liquid chromatography-tandem mass spectrometry to investigate urea cycle disorders. [Journal Article]
- CCClin Chim Acta 2019 May 13; 495:406-416
- Urea cycle disorders (UCD) are inborn errors of ammonia detoxification in which early diagnosis and treatment are critical to prevent metabolic emergencies. Unfortunately, the diagnosis was often and…
Urea cycle disorders (UCD) are inborn errors of ammonia detoxification in which early diagnosis and treatment are critical to prevent metabolic emergencies. Unfortunately, the diagnosis was often and pronounced delayed. To improve diagnosis, we developed herein a liquid chromatography-tandem mass spectrometry method to investigate the disturbance of amino acid profile caused by UCD. The method enabled absolute quantification of 48 amino acids (AAs) within 20 min. Only 2.5 μL plasma was required for the analysis. The lower limits of quantification for most AAs were 0.01 μmol/L. Method accuracies ranged from 89.9% to 113.4%. The within- and between-run coefficients of variation were 0.8-7.7% and 2.6-14.5%, respectively. With this method, age-specific reference values were established for 42 AAs by analyzing 150 samples from normal controls, and patients with different subtypes of UCD were successfully distinguished. The data of patients revealed that UCD not only disturbed the metabolism of urea cycle AAs and induced accumulation of ammonia detoxification AAs, but also interfered the metabolism of some nervous system related AAs, such as pipecolic acid and N-acetylaspartic acid. This data may provide new insight into pathogenesis for UCD.
- [Soil Organic Carbon Distribution and Components in Different Plant Communities Along a Water Table Gradient in the Huixian Karst Wetland in Guilin]. [Journal Article]
- HJHuan Jing Ke Xue 2019 Mar 08; 40(3):1491-1503
- In order to reveal the effect of vegetation type and soil physicochemical properties on the distribution of soil organic carbon and its components, a field survey was carried out on nine different pl…
In order to reveal the effect of vegetation type and soil physicochemical properties on the distribution of soil organic carbon and its components, a field survey was carried out on nine different plant communities along a water table gradient in the Huixian wetland with samples of soil at 0-10 cm, 10-20 cm, and 20-30 cm in depth. The soil organic carbon (SOC), light fraction organic carbon (LFOC), heavy fraction organic carbon (HFOC), easily oxidized organic carbon (EOC), dissolved organic carbon (DOC), particulate organic carbon (POC), and microbial biomass carbon (MBC) were measured. The correlations among soil organic carbon components and soil physicochemical properties were also examined. The results showed that:① The average proportion of LFOC and HFOC to SOC at 0-30 cm soil depth was 11.10% and 88.90%, respectively. The distribution ratio of the heavy component was much higher than of the light component in soils. ② The content of SOC, DOC, EOC, POC, and MBC (except in the Panicum repens community) and the values of DOC/SOC, EOC/SOC, and POC/SOC all decreased with increase of the soil depth. ③ Among the nine different plant communities, the contents of SOC, LFOC, HFOC, MBC, DOC, EOC, and POC of Cladium chinense were significantly higher than for other communities in same soil layers. ④ There were significantly positive correlations among soil organic carbon components (SOC) and soil total nitrogen (TN). LFOC, HFOC, DOC, and POC were also positively correlated with soil pH. The soil bulk density was significantly negative correlated with LFOC, HFOC, DOC, EOC, and POC, and the content of clay was also negatively correlated with LFOC, HFOC, DOC, POC, and MBC. ⑤ Path analysis showed that TN, soil pH, soil sand content, and soil water content (SWC) has indirect effects on HFOC by influencing other soil factors. Soil TN had strong positive effects on EOC, DOC, and POC, and SWC also has the largest direct negative effect on MBC. This showed that there were close interactions between soil physicochemical properties and soil organic carbon components. This study may provide a reference base for sustainable development and scientific predictions regarding the Huixian Karst wetland.
- Biomagnification characteristics and health risk assessment of the neurotoxin BMAA in freshwater aquaculture products of Taihu Lake Basin, China. [Journal Article]
- CChemosphere 2019; 229:332-340
- In freshwater aquaculture ecosystems with high-frequency occurrences of cyanobacteria blooms, a chronic neurotoxic cyanobacteria toxin, β-N-methylamino-l-alanine (BMAA), is a new pollutant that affec…
In freshwater aquaculture ecosystems with high-frequency occurrences of cyanobacteria blooms, a chronic neurotoxic cyanobacteria toxin, β-N-methylamino-l-alanine (BMAA), is a new pollutant that affects the normal growth, development, and reproduction of aquaculture organisms. BMAA poses a great threat to the food quality and food safety of aquatic products. In this paper, high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) was used to detect the contents of BMAA in the edible portions of six representative freshwater aquaculture products (Corbicula fluminea, Anodonta arcaeformis, Macrobrachium nipponense, Eriocheir sinensis, Ctenopharyngodon idella, and Mylopharyngodon piceus) from Taihu Lake Basin in China. Noncarcinogenic health risks were assessed with reference to the model recommended by the International Environmental Modelling and Software Society and based on the biomagnification characteristics of BMAA in the various aquaculture products investigated by the stable nitrogen isotope technique. The average BMAA concentrations in the edible portions of the six freshwater culture products were from 2.05 ± 1.40 to 4.21 ± 1.26 μg g-1 dry weight (DW), and the difference was significant (p < 0.05), such a difference increased with the increase in the trophic level in the aquaculture products. Although a biomagnification indication was observed, the trophic magnification factor (TMF) was only 1.20 which exhibited a relatively low biomagnification efficiency. The annual health risk values of BMAA in all the measured aquatic products were within the maximum tolerable range (<1 × 10-6 a-1), and the health risk increased with the increase in the trophic level. The risk values of BMAA in the six freshwater aquaculture products for children was slightly higher than the negligible level (<1 × 10-7 a-1), thus there might have potential health risks for children's long-term consumption. Considering China's national conditions, the guidance values of BMAA based on the quality and safety of freshwater aquaculture products were proposed to be 7.2 μg g-1 DW for adults and 1.8 μg g-1 DW for children.
- Baseline haematological and biochemical reference values for healthy male adults from Mali. [Journal Article]
- PAPan Afr Med J 2019; 32:5
- CONCLUSIONS: This is the first study to determine normal laboratory parameters in Malian adult males. Our results underscore the necessity of establishing region-specific clinical reference ranges that would allow clinicians and practitioners to manage laboratory tests, diagnosis and therapies. These data are useful not only for the management of patients in Mali, but also to support European and American clinicians in the health management of asylum seekers and migrants from Mali.
- A seriously sand storm mixed air-polluted area in the margin of Tarim Basin: Temporal-spatial distribution and potential sources. [Journal Article]
- STSci Total Environ 2019 Aug 01; 676:436-446
- In order to analyze the temporal-spatial distribution characteristics of PM2.5, PM10, SO2, NO2, CO and O3 in five cities and the potential sources of PM10 in southern Xinjiang during 2016, we collect…
In order to analyze the temporal-spatial distribution characteristics of PM2.5, PM10, SO2, NO2, CO and O3 in five cities and the potential sources of PM10 in southern Xinjiang during 2016, we collected one year officially released data for analysis. The average PM10, PM2.5, SO2, NO2, O3 and CO concentrations were 289 ± 363, 99 ± 106, 17 ± 9, 29 ± 11, 65 ± 25 μg m-3 and 1.3 ± 0.6 mg m-3 in southern Xinjiang in 2016, respectively. The air pollutants presented distinct seasonal and spatial distribution characteristics. During sandstorm process, the particulate matters (PM) concentrations increased abruptly, with the PM10 and PM2.5 maximum concentrations exceeding 1000 and 500 μg m-3 in each city. The backward trajectory results showed that the air masses in Akesu, Kurla, Hotan, Kashi and Atushi were mainly from the Bayingol Mongolian Autonomous Prefecture, Kyrgyzstan, Kizilesu Kirgiz Autonomous Prefecture and Taklimakan Desert (TD). In addition, TD was the main potential contributor to ambient PM10 in five cities during the dust season (DS), with a weighted potential source contribution function (WPSCF) > 0.9. While the trajectories of air masses from TD, Bayingol Mongolian Autonomous Prefecture, Urumqi-Changji Area and local emission were potential sources contributing to PM2.5 in these five cities during DS, with a WPSCF > 0.7. Moreover, the high weighted concentration weighted trajectory (WCWT) values were distributed in the Tarim basin, with PM10 > 700 μg m-3, however, the local emission and long distance transport contributed to the PM2.5 > 160 μg m-3 for five cities. This study comprehensively analyzes the pollution characteristics of air pollutants in five important cities in the southern margin of the Tarim Basin for the first time, and will provide an important reference basis for the prevention and control of air pollution in southern Xinjiang.
- A Novel Mathematical Model for Correcting the Physiologic Variance of Two-Dimensional Echocardiographic Measurements in Healthy Chinese Adults. [Journal Article]
- JAJ Am Soc Echocardiogr 2019 Apr 24
- CONCLUSIONS: The novel optimized multivariate allometric model developed in this study is superior to traditional the single-variable isometric model in the correction of echocardiographic parameters for physiologic effects of age, gender, and body size variables and thus should be encouraged in both scientific research and clinical practice.
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- Simultaneous determination of nine trace concentration angiotensin peptides in human serum using ultra high performance liquid chromatography with tandem mass spectrometry with sephadex LH-20 gel solid-phase extraction. [Journal Article]
- JSJ Sep Sci 2019 Apr 25
- The renin-angiotensin system is a highly complex enzymatic system consisting of multiple peptide hormones, enzymes, and receptors. Here, an assay to simultaneously quantify eight angiotensin peptides…
The renin-angiotensin system is a highly complex enzymatic system consisting of multiple peptide hormones, enzymes, and receptors. Here, an assay to simultaneously quantify eight angiotensin peptides and bradykinin in human serum was developed and validated, using ultra high performance liquid chromatography coupled with tandem mass spectrometry. A pre-concentration method of Sephadex LH-20 gel solid-phase extraction was first applied for analysis of angiotensin peptides from serum sample. The triple quadrupole mass spectrometer was operated in the positive ion mode and multiple reaction monitoring was used for drug quantification. The analytical time was within 5 min, much raising the analysis efficiency. Limits of detection ranged from 0.9 to 1.3 pg/mL, and displayed the same level of sensitivity compared with radioimmunoassay. The method was successfully applied to 22 healthy human serum samples, giving the concentrations of angiotensin I, angiotensin II, angiotensin III, angiotensin IV, angiotensin 1-9, angiotensin 1-7, angiotensin 1-5, Asn1 ,Val5 -Angiotensin II, and bradykinin for reference. This novel metabolic profile study of vasoactive peptides based on gel solid-phase extraction concentration provided not only an accurate quantitative assay of the serum concentrations, but also a promising methodology for evaluating the diagnostic values of the various peptides.