- Pharmacological characterization of the seven human NOX isoforms and their inhibitors. [Journal Article]
- RBRedox Biol 2019 Jul 11; 26:101272
- CONCLUSIONS: Our findings indicate that experimental results obtained with widely used NOX inhibitors must be carefully interpreted and highlight the challenge of developing reliable pharmacological inhibitors of these key molecular targets.
- Simultaneous determination of phenolic acids and flavonoids in Artemisiae Argyi Folium by HPLC-MS/MS and discovery of antioxidant ingredients based on relevance analysis. [Journal Article]
- JPJ Pharm Biomed Anal 2019 Jul 05; 175:112734
- A rapid and reliable LC-MS/MS method was developed for the quantitation of major components in Folium Artemisiae Argyi (mugwort), a widely used traditional Chinese herbal medicine. A total of 5 pheno…
A rapid and reliable LC-MS/MS method was developed for the quantitation of major components in Folium Artemisiae Argyi (mugwort), a widely used traditional Chinese herbal medicine. A total of 5 phenolic acids and 17 flavonoids were separated and simultaneously determined by using a Shiseido C18 column (150 × 3.0 mm, 3 μm) and gradient elution of acetonitrile-aqueous formic acid (100:0.1, v/v) at a 0.5 mL min-1 flow rate, via multiple reaction monitor (MRM) in polarity switching mode. The quantitative method was validated in terms of sensitivity, linearity, precision, accuracy and stability, which proved to be sensitive, accurate and reproducible. Then 65 samples collected from different areas were selected for component analysis by LC-MS/MS and assessment of antioxidant activity using DPPH, ABTS, FRAP, O2- and OH scavenging assays. Grey relational analysis and partial least square regression were used to evaluate the relevance between chemicals and bioactivities, and the results indicated chlorogenic acid, isochlorogenic acid B, A, C, eriodictyol, jaceosidin and eupatilin made the key contribution to antioxidant activity. The present study combines chemical analysis and bioassay to identify bioactive markers, which possesses potential value for the activity-oriented quality control of mugwort.
- Self-Limiting Processes in the Flame-Based Fabrication of Superhydrophobic Surfaces from Silicones. [Journal Article]
- AAACS Appl Mater Interfaces 2019 Jul 22
- Outdoor applications of superhydrophobic coatings require synthetic approaches that allow their simple, fast, scalable, and environmentally benign deployment on large, heterogeneous surfaces, and the…
Outdoor applications of superhydrophobic coatings require synthetic approaches that allow their simple, fast, scalable, and environmentally benign deployment on large, heterogeneous surfaces, and their rapid regeneration in situ. We recently showed that the thermal degradation of silicones by flames fulfills these characteristics by spontaneously structuring silicone surfaces into a hierarchical, textured structure that provides wear-resistant, healable superhydrophobicity. This paper elucidates how flame processing - a simple, rapid, and out-of-equilibrium process - can be so counterintuitively reliable and robust in producing such a complex structure. A comprehensive study of the effect of the processing speed and flame temperature on the chemical and physical properties of the coatings yielded three surprising results. (i) Three thermal degradation mechanisms drive the surface texturing: depolymerization (in the O2-rich conditions of the surface), decomposition (in the O2-poor conditions found few microns from the surface), and pyrolysis at excessive temperatures. (ii) The operational condition is delimited by the onset of the depolymerization at low temperatures, and the onset of pyrolysis at high temperatures. (iii) The remarkably wide operational conditions and robustness of this approach results from self-limiting growth and oxidation of the silicone particles that are are responsible for the surface texturing and in the extent of their deposition. As a result of this analysis we show that superhydrophobic surfaces can be produced or regenerated with this approach at a speed of 15 cm·s-1 (i.e., the length of an airport runway in ~4.5 hrs).
- Netrin-1 Dampens Hypobaric Hypoxia-Induced Lung Injury in Mice. [Journal Article]
- HAHigh Alt Med Biol 2019 Jul 22
- CONCLUSIONS: Netrin-1 dampens hypobaric hypoxia-induced lung injury by inhibiting neutrophil migration and attenuating apoptosis.
- Tomato GLR3.3 and GLR3.5 mediate cold acclimation-induced chilling tolerance by regulating apoplastic H2 O2 production and redox homeostasis. [Journal Article]
- PCPlant Cell Environ 2019 Jul 22
- Plant GLUTAMATE RECEPTOR-LIKE (GLR) genes play important roles in plant development and immune response. However, the functions of GLRs in abiotic stress response remain unclear. Here we show that co…
Plant GLUTAMATE RECEPTOR-LIKE (GLR) genes play important roles in plant development and immune response. However, the functions of GLRs in abiotic stress response remain unclear. Here we show that cold acclimation at 12°C induced the transcripts of GLR3.3 and GLR3.5 with increased tolerance against a subsequent chilling at 4 °C. Silencing of GLR3.3 or/and GLR3.5 or application of the antagonist of ionotropic glutamate receptor 6,7-dinitroquinoxaline-2,3-dione (DNQX), all compromised the acclimation-induced increases in the transcripts of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1), activity of NADPH oxidase, the accumulation of apoplastic H2 O2 and the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG), resulting in an attenuated chilling tolerance, the effect, however, was rescued by foliar application of H2 O2 or GSH. Both RBOH1-silenced and glutathione biosynthesis genes, γ-GLUTAMYLCYSTEINE SYNTHETASE (GSH1)- and GLUTATHIONE SYNTHETASE (GSH2)-cosilenced plants had decreased chilling tolerance with reduced GSH/GSSG ratio. Moreover, application of DNQX had little effects on the GSH/GSSG ratio and the tolerance in RBOH1-silenced plants and GSH1- and GSH2-cosilenced plants. These findings unmasked the functional hierarchy of GLR-H2 O2 -Glutathione cascade and shed new light on cold response pathway in tomato plants.
- Effects of chalcones on NADPH oxidaseChalcone skeleton promotes transcription of gp91-phox gene but inhibits expression of gp91-phox protein, and hydroxyl groups in hydroxychalcones participate in the stable expression of gp91-phox protein. [Journal Article]
- MIMicrobiol Immunol 2019 Jul 22
- We studied the effects of chalcone and butein on the induction of the superoxide anion (O2 -)-generating system in U937 cells by all-trans retinoic acid (RA). Chalcone skeleton, a common structural m…
We studied the effects of chalcone and butein on the induction of the superoxide anion (O2 -)-generating system in U937 cells by all-trans retinoic acid (RA). Chalcone skeleton, a common structural motif in them, significantly enhanced transcription of gp91-phox in an epigenetic manner. In contrast, chalcone and butein showed the opposite effects on induction of the O2 - -generating activity by RA and expression of gp91-phox protein. Chalcone inhibited whereas butein promoted the induction of O2 - -generating activity by RA and the expression of gp91-phox protein. Our data raise the possibility that modification of chalcone skeleton can produce more effective differentiation-promoting agents. This article is protected by copyright. All rights reserved.
- Large-Area, Uniform, Aligned Arrays of Na3 (VO)2 (PO4)2 F on Carbon Nanofiber for Quasi-Solid-State Sodium-Ion Hybrid Capacitors. [Journal Article]
- SSmall 2019 Jul 22; :e1902466
- Sodium-vanadium fluorophosphate (Na3 V2 O2 x (PO4)2 F3-2 x , NVPF, 0 ≤ x ≤ 1) is considered to be a promising Na-storage cathode material due to its high operation potentials (3.6-4 V) and minor volu…
Sodium-vanadium fluorophosphate (Na3 V2 O2 x (PO4)2 F3-2 x , NVPF, 0 ≤ x ≤ 1) is considered to be a promising Na-storage cathode material due to its high operation potentials (3.6-4 V) and minor volume variation (1.8%) during Na+ -intercalation. Research about NVPF is mainly focused on powder-type samples, while its ordered array architecture is rarely reported. In this work, large-area and uniform Na3 (VO)2 (PO4)2 F cuboid arrays are vertically grown on carbon nanofiber (CNF) substrates for the first time. Owing to faster electron/ion transport and larger electrolyte-electrode contact area, the as-prepared NVPF array electrode exhibits much improved Na-storage properties compared to its powder counterpart. Importantly, a quasi-solid-state sodium-ion hybrid capacitor (SIHC) is constructed based on the NVPF array as an intercalative battery cathode and porous CNF as a capacitive supercapacitor anode together with the P(VDF-HFP)-based polymer electrolyte. This novel hybrid system delivers an attractive energy density of ≈227 W h kg-1 (based on total mass of two electrodes), and still remains as high as 107 Wh kg-1 at a high specific power of 4936 W kg-1 , which pushes the energy output of sodium hybrid capacitors toward a new limit. In addition, the growth mechanism of NVPF arrays is investigated in detail.
- Synthesis of CaO2 Nanocrystals and Their Spherical Aggregates with Uniform Sizes for Use as a Biodegradable Bacteriostatic Agent. [Journal Article]
- SSmall 2019 Jul 22; :e1902118
- As a solid precursor to O2 and hydrogen peroxide (H2 O2), calcium peroxide (CaO2) has found widespread use in applications related to disinfection and contaminant degradation. The lack of uniform nan…
As a solid precursor to O2 and hydrogen peroxide (H2 O2), calcium peroxide (CaO2) has found widespread use in applications related to disinfection and contaminant degradation. The lack of uniform nanoparticles, however, greatly limits the potential use of this material in other applications related to medicine. Here, a new route to the facile synthesis of CaO2 nanocrystals and their spherical aggregates with uniform, controllable sizes is reported. The synthesis involves the reaction between CaCl2 and H2 O2 to generate CaO2 primary nanocrystals of 2-15 nm in size in ethanol, followed by their aggregation into uniform, spherical particles with the aid of poly(vinyl pyrrolidone) (PVP). The average diameter of the spherical aggregates can be easily tuned in the range of 15-100 nm by varying the concentrations of CaCl2 and/or PVP. For the spherical aggregates with a smaller size, they release H2 O2 and O2 more quickly when exposed to water, resulting in superior antimicrobial activity. This study not only demonstrates a new route to the synthesis of uniform CaO2 nanocrystals and their spherical aggregates but also offers a promising bacteriostatic agent with biodegradability.
- Mononuclear Manganese(III) Superoxo Complexes: Synthesis, Characterization, and Reactivity. [Journal Article]
- ICInorg Chem 2019 Jul 22
- Metal-superoxo species are typically proposed as key intermediates in the catalytic cycle of dioxygen activation by metalloenzymes involving different transition metal cofactors. In this regard, whil…
Metal-superoxo species are typically proposed as key intermediates in the catalytic cycle of dioxygen activation by metalloenzymes involving different transition metal cofactors. In this regard, while a series of Fe-, Co-, and Ni-superoxo complexes have been reported to date, well-defined Mn-superoxo complexes remain rather rare. Herein, we report two mononuclear MnIII-superoxo species, Mn(BDPP)(O2•-) (2, H2BDPP = 2,6-bis((2-(S)-diphenylhydroxylmethyl-1-pyrrolidinyl)methyl)pyridine) and Mn(BDPBrP)(O2•-) (2', H2BDPBrP = 2,6-bis((2-(S)-di(4-bromo)phenylhydroxyl-methyl-1-pyrrolidinyl)methyl)pyridine), synthesized by bubbling O2 into solutions of their MnII precursors, Mn(BDPP) (1) and Mn(BDPBrP) (1'), at -80 °C. A combined spectroscopic (resonance Raman and electron paramagnetic resonance (EPR) spectroscopy) and computational study evidence that both complexes contain a high-spin MnIII center (SMn = 2) antiferromagnetically coupled to a superoxo radical ligand (SOO• = 1/2), yielding an overall S = 3/2 ground state. Complexes 2 and 2' were shown to be capable of abstracting a H atom from 2,2,6,6-tetramethyl-1-hydroxypiperidine (TEMPO-H) to form MnIII-hydroperoxo species, Mn(BDPP)(OOH) (5) and Mn(BDPBrP)(OOH) (5'). Complexes 5 and 5' can be independently prepared by the reactions of the isolated MnIII-aqua complexes, [Mn(BDPP)(H2O)]OTf (6) and [Mn(BDPBrP)(H2O)]OTf (6'), with H2O2 in the presence of NEt3. The parallel-mode EPR measurements established a high-spin S = 2 ground state for 5 and 5'.
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- Effects of exercise training with short-duration intermittent hypoxia on endurance performance and muscle metabolism in well-trained mice. [Journal Article]
- PRPhysiol Rep 2019; 7(14):e14182
- The author previously reported that short-duration intermittent hypoxia had additive effects on improvements in endurance capacity by enhancing fatty acid metabolism. The present study was designed t…
The author previously reported that short-duration intermittent hypoxia had additive effects on improvements in endurance capacity by enhancing fatty acid metabolism. The present study was designed to investigate the effects of short-duration intermittent hypoxia on endurance capacity, metabolic enzyme activity, and protein levels associated with mitochondrial biogenesis in well-trained mice. Mice in the training group were housed in a cage with a running wheel for 7 weeks from 5 weeks old. Voluntary running markedly increased maximal work values by 5.0-fold. Trained mice were then subjected to either endurance treadmill training (ET) for 60 min or hybrid training (HT, ET for 30 min followed by sprint interval exercise (5-sec run-10-sec rest) for 30 min) with (H-ET or H-HT) or without (ET or HT) short-duration intermittent hypoxia (4 cycles of 12-13% O2 for 15 min and 20.9% O2 for 10 min) for 4 weeks. Maximal endurance capacity was markedly greater in the H-ET and H-HT than ET and HT groups, respectively. H-ET and H-HT increased activity levels of 3-hydroxyacyl-CoA-dehydrogenase in oxidative muscle portion and pyruvate dehydrogenase complex in glycolytic muscle portion. These activity levels were significantly correlated with maximal endurance capacity. Protein levels of dynamin-related protein-1 were increased more by H-ET and H-HT than by ET and HT, but were not significantly correlated with maximal work. These results suggest that intermittent hypoxic exposure has beneficial effects on endurance and hybrid training to improve the endurance capacity via improving fatty acid and pyruvate oxidation in highly trained mice.