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Unbound Medicine.
(Spirochaeta)
862 results
  • Allosteric conformational change of a cyclic nucleotide-gated ion channel revealed by DEER spectroscopy. [Journal Article]
    Proc Natl Acad Sci U S A. 2020 May 19; 117(20):10839-10847.Evans EGB, Morgan JLW, … Stoll S
  • Cyclic nucleotide-gated (CNG) ion channels are essential components of mammalian visual and olfactory signal transduction. CNG channels open upon direct binding of cyclic nucleotides (cAMP and/or cGMP), but the allosteric mechanism by which this occurs is incompletely understood. Here, we employed double electron-electron resonance (DEER) spectroscopy to measure intersubunit distance distribution…
  • Complete Genome Sequences and Methylome Analysis of Two Environmental Spirochaetes. [Journal Article]
    Microbiol Resour Announc. 2020 Apr 09; 9(15)Fomenkov A, Grabovich MY, … Roberts RJ
  • Here, we report the finished closed genomes of two environmental bacteria, Oceanispirochaeta crateria K2 and Thiospirochaeta perfilievii P (formally known as Spirochaeta perfilievii P). In addition, we provide methylation data and the associated enzymes predicted and confirmed to be responsible for each modified motif.
  • Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii. [Journal Article]
    Front Microbiol. 2020; 11:308.Schwob G, Cabrol L, … Orlando J
  • Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bac…
  • Metabolic engineering of Clostridium thermocellum for n-butanol production from cellulose. [Journal Article]
    Biotechnol Biofuels. 2019; 12:186.Tian L, Conway PM, … Lynd LR
  • CONCLUSIONS: Here we report engineering C. thermocellum to produce n-butanol. Our initial pathway designs resulted in low levels (88 mg/L) of n-butanol production. By engineering the protein sequence of key enzymes in the pathway, we increased the n-butanol titer by 2.2-fold. We further increased n-butanol production by adding ethanol to the growth media. By combining all these improvements, the engineered strain was able to produce 357 mg/L of n-butanol from cellulose within 120 h.
  • Phyloproteomic and functional analyses do not support a split in the genus Borrelia (phylum Spirochaetes). [Journal Article]
    BMC Evol Biol. 2019 02 13; 19(1):54.Estrada-Peña A, Cabezas-Cruz A
  • CONCLUSIONS: We found only marginal functional differences among Borrelia species. Phyloproteomic networks that included all pairwise combinations between species, proteins, and processes were more effective than other methods for evaluating the evolutionary relationships among taxa. With the limitations of data availability, our results did not support a split of the arthropod-transmitted spirochaetes into the proposed genera, Borrelia and Borreliella.
  • The chemodiversity of paddy soil dissolved organic matter correlates with microbial community at continental scales. [Journal Article]
    Microbiome. 2018 10 19; 6(1):187.Li HY, Wang H, … Zhang ZJ
  • CONCLUSIONS: Our study demonstrates the continental-scale distribution of DOM is significantly correlated with the taxonomic profile and metabolic potential of the rice paddy microbiome. Abiotic factors that have a distinct effect on community structure can also influence the chemodiversity of DOM and vice versa. Deciphering these associations and the underlying mechanisms can precipitate understanding of the complex ecology of paddy soils, as well as help assess the effects of human activities on biogeochemistry and greenhouse gas emissions in paddy soils.
  • An iris diaphragm mechanism to gate a cyclic nucleotide-gated ion channel. [Journal Article]
    Nat Commun. 2018 09 28; 9(1):3978.Marchesi A, Gao X, … Scheuring S
  • Cyclic nucleotide-gated (CNG) ion channels are non-selective cation channels key to signal transduction. The free energy difference of cyclic-nucleotide (cAMP/cGMP) binding/unbinding is translated into mechanical work to modulate the open/closed probability of the pore, i.e., gating. Despite the recent advances in structural determination of CNG channels, the conformational changes associated wit…
  • Enhancement of synchronous bio-reductions of vanadium (V) and chromium (VI) by mixed anaerobic culture. [Journal Article]
    Environ Pollut. 2018 Nov; 242(Pt A):249-256.Wang S, Zhang B, … Liu H
  • The co-occurrence of toxic vanadium (V) and chromium (VI) in groundwater receives incremental attention while knowledge on their interactions in biogeochemical processes is limited, with lack of efficient removal means. This study is the first to realize synchronous bio-reductions of V(V) and Cr(VI) with high efficiency by mixed anaerobic culture. After 72-h operation, 97.0 ± 1.0% of V(V) and 99.…
  • A Spirochaete is suggested as the causative agent of Akoya oyster disease by metagenomic analysis. [Journal Article]
    PLoS One. 2017; 12(8):e0182280.Matsuyama T, Yasuike M, … Nakayasu C
  • Mass mortality that is acompanied by reddish browning of the soft tissues has been occurring in cultured pearl oyster, Pinctada fucata martensii. The disease is called Akoya oyster disease (AOD). Although spreading pattern of the disease and transmission experiments suggest that the disease is infectious, the causative agent has not yet been identified. We used shotgun and 16S rRNA-based metageno…
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