- Severe Obesity, Obesity, and Cardiometabolic Risk in Children 0 to 6 Years of Age. [Journal Article]
- COChild Obes 2017; 13(5):415-424
- CONCLUSIONS: Young children with a zBMI >3 have significantly higher blood pressure measures and trends toward worse lipid profiles than children at lower zBMIs.
- Effect of high-density lipoprotein on oocyte maturation and bovine embryo development in vitro. [Journal Article]
- RDReprod Domest Anim 2018 Nov 12
- High-density lipoprotein (HDL) is the main lipoprotein in the follicular fluid and it has anti-inflammatory, antioxidant and cryoprotectant properties. The anti-inflammatory and antioxidant potential...
High-density lipoprotein (HDL) is the main lipoprotein in the follicular fluid and it has anti-inflammatory, antioxidant and cryoprotectant properties. The anti-inflammatory and antioxidant potential are derived from its lipid composition, especially the apolipoprotein AI (ApoAI) and paraoxonase 1 (PON1). The aim of this study was to evaluate the effect of HDL during in vitro maturation (IVM) on oocyte maturation and early bovine embryo development. For this, cumulus oocyte complexes (COCs) were obtained from bovine ovaries collected at a local slaughterhouse. COCs (n=2250) were allocated into three groups (n=50 COCs/group) according to the addition of HDL protein (HDL-P) during IVM for 22 h: 0 mg/dL (control), 50 mg/dL and 150 mg/dL. After IVM, COCs were inseminated (IVF) and cultivated for 7 days. Total cholesterol concentration, total protein, triglycerides and ApoAI concentrations on IVM medium increased proportionally to HDL-P addition. However, PON1 activity was not detected in any treatment. The addition of HDL-P did not affect nuclear maturation rate, endogenous reactive oxygen species and glutathione levels in COCs (P > 0.05). The highest HDL-P concentration (150 mg/dL) decreased cleavage and blastocyst rate (P < 0.05). Moreover, the HDL-P 150 mg/dL group had lower cellular count/blastocyst than the 50 mg/dL group (P < 0.05). However, the addition of HDL-P did not affect relative gene expression of evaluated genes. In conclusion, the complex HDL/ApoAI obtained from human plasma, in absence of PON1 activity during in vitro oocyte maturation, decreased initial embryo development. This article is protected by copyright. All rights reserved.
- Effect of pennyroyal, savory and thyme essential oils on Japanese quail physiology. [Journal Article]
- HHeliyon 2018; 4(10):e00881
- An experiment was conducted to show the effects of different levels of pennyroyal, thyme and savory essential oils dietary supplementation on performance, organs weight, intestinal morphology, and se...
An experiment was conducted to show the effects of different levels of pennyroyal, thyme and savory essential oils dietary supplementation on performance, organs weight, intestinal morphology, and serum lipids in quails. A total of 550 day-old Japanese quail chicks were allocated into 11 dietary treatments of 5 replications (10 birds in each cage) under a randomized experimental design. The treatments were a basal diet (Control), or 3 levels (200, 300, and 400 ppm) of thyme essential oil (TO), or 3 levels (200, 300, and 400 ppm) of savory essential oil (SO), or 3 levels (200, 300, and 400 ppm) of pennyroyal essential oil (PO) added separately to the basal diet, or also the basal diet supplemented with 100 ppm of flavophospholipol. Body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) were measured weekly and calculated totally for 35 d. The organs weight and morphology of intestine parts of one bird from each cage were measured on 35 d. At the same day, blood samples were collected and cholesterol and triglyceride values were separately determined for male and female quails. Although the addition of different levels of essential oils in quail's diet did not affect BWG, a significant decline in FI was observed in the group supplemented with 400 ppm of TO. At the same time, FCR significantly improved (P < 0.05) in the same group as well as in the group supplemented with the antibiotic. However, the organs weight was not significantly affected by the experimental treatments. Nevertheless, villi height of duodenum, jejunum and ileum was significantly increased and crypt depth was significantly decreased in the quails fed diets supplemented with different levels of TO and SO. The values of serum triglycerides decreased in both sexes in the groups that received diets supplemented with different levels of essential oils whereas the values of cholesterol decreased only in males of the essential oils supplemented groups. From the present observations it can be concluded that thyme and savory essential oils can improve FCR by decreasing FI through boosting the absorption of nutrients in intestine. These plant essential oils can replace antibiotic growth parameters without having any adverse effect on quail's health with thyme essential oil exerting the most effective activity.
- N-Acetylcysteine (NAC) Ameliorates Lipid-Related Metabolic Dysfunction in Bone Marrow Stromal Cells-Derived Adipocytes. [Journal Article]
- EBEvid Based Complement Alternat Med 2018; 2018:5310961
- Recent experimental data suggest that fatty acids and lipotoxicity could play a role in the initiation and evolution of metabolic bone diseases such as osteoporosis. A functional bone marrow adipose ...
Recent experimental data suggest that fatty acids and lipotoxicity could play a role in the initiation and evolution of metabolic bone diseases such as osteoporosis. A functional bone marrow adipose tissue (BMAT) may provide support to surrounding cells and tissues or may serve as a lipid reservoir that protects skeletal osteoblasts from lipotoxicity. The present study examined the effect of N-acetylcysteine (NAC), a powerful antioxidant and precursor of glutathione, commonly used to treat chronic obstructive pulmonary disease, on triglycerides accumulation in bone marrow stromal cells-derived adipocytes. Quantification of Oil Red O stained cells showed that lipid droplets decreased following NAC treatment. Additionally, exposure of bone marrow stromal cells (HS-5) to NAC increased adiponectin, PPARγ, HO-1, and SIRT-1 and increased beta-oxidation markers such as PPARα and PPARδ mRNA levels. As there is now substantial interest in alternative medicine, the observed therapeutic value of NAC should be taken into consideration in diabetic patients.
- The Effects of Folic Acid Supplementation on Recurrence and Metabolic Status in Endometrial Hyperplasia: A Randomized, Double-Blind, Placebo-Controlled Trial. [Journal Article]
- AIArch Iran Med 2018 Oct 01; 21(10):452-459
- CONCLUSIONS: In conclusion, we found that folic acid administration for 12 weeks to subjects with EH improved glycemic control, triglycerides, VLDL-cholesterol and hs-CRP levels, but did not influence recurrence and other metabolic profiles.
- Energy and metabolic pathways in trefoil factor family member 2 (Tff2) KO mice beyond the protection from high-fat diet-induced obesity. [Journal Article]
- LSLife Sci 2018 Nov 07
- CONCLUSIONS: We mapped a part of the metabolic and biochemical pathways of lipids and glucose involving the adipose tissue, liver, skeletal muscle and sympathetic nervous system that protect Tff2 KO mice from the HF diet-induced obesity. Our data highlight Tff2-related pathways as potential targets for obesity therapies.
- Comparative effects of dietary n-3 docosapentaenoic acid (DPA), DHA and EPA on plasma lipid parameters, oxidative status and fatty acid tissue composition. [Journal Article]
- JNJ Nutr Biochem 2018 Oct 12; 63:186-196
- The specific and shared physiologic and metabolic effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and even more of n-3 docosapentaenoic acid (DPA) are poorly known. We investigated...
The specific and shared physiologic and metabolic effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and even more of n-3 docosapentaenoic acid (DPA) are poorly known. We investigated the physiological effects and the overall fatty acid tissue composition of a nutritional supplementation of DPA compared both to EPA and DHA in healthy adult rats. Rats (n=32) were fed with semisynthetic diets supplemented or not with 1% of total lipids as EPA, DPA or DHA in ethyl esters form from weaning for 6 weeks. Fatty acid tissue composition was determined by gas chromatography-mass spectrometry, and blood assays were performed. The DPA supplementation was the only one that led to a decrease in plasma triglycerides, total cholesterol, non-high-density lipoprotein (HDL)-cholesterol, cholesterol esters and total cholesterol/HDL-cholesterol ratio compared to the nonsupplemented control group. The three supplemented groups had increased plasma total antioxidant status and superoxide dismutase activity. In all supplemented groups, the n-3 polyunsaturated fatty acid level increased in all studied tissues (liver, heart, lung, spleen, kidney, red blood cells, splenocytes, peripheral mononucleated cells) except in the brain. We showed that the DPA supplementation affected the overall fatty acid composition and increased DPA, EPA and DHA tissue contents in a similar way than with EPA. However, liver and heart DHA contents increased in DPA-fed rats at the same levels than in DHA-fed rats. Moreover, a large part of DPA seemed to be retroconverted into EPA in the liver (38.5%) and in the kidney (68.6%). In addition, the digestibility of DPA was lower than that of DHA and EPA.
- Effects of short term hypothyroidism on the lipid transfer to HDL and other parameters related to lipoprotein metabolism in patients submitted to thyroidectomy for thyroid cancer. [Journal Article]
- TThyroid 2018 Nov 09
- CONCLUSIONS: In short-term hypothyroidism, HDL-cholesterol increased but this did not increase the capacity of the HDL fraction to receive lipids or the activity of PON-1, the anti-oxidation enzyme associated to HDL.
- Cerebrovascular Pathology in Hypertriglyceridemic APOB-100 Transgenic Mice. [Journal Article]
- FCFront Cell Neurosci 2018; 12:380
- Hypertriglyceridemia is not only a serious risk factor in the development of cardiovascular diseases, but it is linked to neurodegeneration, too. Previously, we generated transgenic mice overexpressi...
Hypertriglyceridemia is not only a serious risk factor in the development of cardiovascular diseases, but it is linked to neurodegeneration, too. Previously, we generated transgenic mice overexpressing the human APOB-100 protein, a mouse model of human atherosclerosis. In this model we observed high plasma levels of triglycerides, oxidative stress, tau hyperphosphorylation, synaptic dysfunction, cognitive impairment, increased neural apoptosis and neurodegeneration. Neurovascular dysfunction is recognized as a key factor in the development of neurodegenerative diseases, but the cellular and molecular events linking cerebrovascular pathology and neurodegeneration are not fully understood. Our aim was to study cerebrovascular changes in APOB-100 transgenic mice. We described the kinetics of the development of chronic hypertriglyceridemia in the transgenic animals. Increased blood-brain barrier permeability was found in the hippocampus of APOB-100 transgenic mice which was accompanied by structural changes. Using transmission electron microscopy, we detected changes in the brain capillary endothelial tight junction structure and edematous swelling of astrocyte endfeet. In brain microvessels isolated from APOB-100 transgenic animals increased Lox-1, Aqp4, and decreased Meox-2, Mfsd2a, Abcb1a, Lrp2, Glut-1, Nos2, Nos3, Vim, and in transgenic brains reduced Cdh2 and Gfap-σ gene expressions were measured using quantitative real-time PCR. We confirmed the decreased P-glycoprotein (ABCB1) and vimentin expression related to the neurovascular unit by immunostaining in transgenic brain sections using confocal microscopy. We conclude that in chronic hypertriglyceridemic APOB-100 transgenic mice both functional and morphological cerebrovascular pathology can be observed, and this animal model could be a useful tool to study the link between cerebrovascular pathology and neurodegeneration.
New Search Next
- Dietary encapsulated probiotic effect on broiler serum biochemical parameters. [Journal Article]
- VWVet World 2018; 11(9):1344-1348
- CONCLUSIONS: It may be concluded that supplementation of the encapsulated probiotic bacteria either alone or in combination significantly increased total serum protein, albumin, and globulin and significantly lowered mean total serum cholesterol, serum LDL cholesterol, and serum triglycerides as compared to the birds that were not supplemented with any probiotic or supplemented with non-encapsulated bacteria.