- The analytical prognosis of the susceptibility to aminoglycosides and doxycycline inacinetobacter baumanniiisoolated from burns of intensive care unit patients. [Journal Article]
- WLWiad Lek 2018; 71(3 pt 2):705-709
- CONCLUSIONS: Conclusion: A.baumannii, pathogens of infectious complications in patiens, were characterized by a decrease in sensitivity to gentamycin, tobramycin and amikacin. Despite of low efficacy rate of doxycycline the recovery of its effectiveness against A.baumannii is excepted.
- [Monoclonal spread of multi-drug resistant CTX-M-15-producing Klebsiella pneumoniae. Impact of measures to control the outbreak]. [Journal Article]
- RERev Esp Quimioter 2018 May 18
- CONCLUSIONS: We detected a monoclonal outbreak of MDR-CTX-M-15-KPN in 2015, with predominance of health-care associated cases. The success in the rapid spread of the outbreak was due to the delay in its detection and to the fact that most of the patients had previously received antibiotics. The control measures reduced the number of isolates by 50%.
- Detection of Acetyltransferase Modification of Kanamycin, an Aminoglycoside Antibiotic, in Bacteria Using Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry. [Journal Article]
- RCRapid Commun Mass Spectrom 2018 May 20
- CONCLUSIONS: A newly developed analytical method is able to determine bacterial resistance to aminoglycosides (via acetylation of kanamycin), qualitatively and quantitatively, within 30 minutes and six hours of incubation with kanamycin, respectively. High-resolution data supports placement of an acetyl group on kanamycin confirming aminoglycoside resistance and its mechanism. Quantification was achieved for both forms of the antibiotic 50-100 fold lower than the minimum inhibitory concentration for the resistant bacteria and can be used to replace conventional antimicrobial susceptibility tests (ASTs).
- Is MIC increase of meropenem against Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae correlated with the increase of resistance rates against some other antibiotics with Gram-negative activity? [Journal Article]
- JGJ Glob Antimicrob Resist 2018 May 15
- CONCLUSIONS: The overall susceptibility rates of antibiotics with Gram-negative activity may greatly vary among KPC-Kp clinical isolates. A tight relationship between meropenem MIC increase and the resistance rate for amikacin was documented.
- Structure-Based Design of a Eukaryote-Selective Antiprotozoal Fluorinated Aminoglycoside. [Journal Article]
- CChemMedChem 2018 May 15
- Aminoglycosides (AG) are antibiotics that lower the accuracy of protein synthesis by targeting a highly-conserved RNA helix of the ribosomal A-site. The discovery of AGs that selectively target the e...
Aminoglycosides (AG) are antibiotics that lower the accuracy of protein synthesis by targeting a highly-conserved RNA helix of the ribosomal A-site. The discovery of AGs that selectively target the eukaryotic ribosome, but lack activity in prokaryotes, are promising as antiprotozoals for the treatment of neglected tropical diseases, and as therapies to read-through point-mutation genetic diseases. However, a single nucleobase change A1408G in the eukaryotic A-site leads to negligible affinity for most AGs. Herein we report the synthesis of 6'-fluoro sisomicin, the first 6'-fluorinated aminoglycoside, which specifically interacts with the protozoal cytoplasmic rRNA A-site, but not the bacterial A-site, as evidenced by X-ray co-crystal structures. The respective dispositions of 6'-F sisomicin within the bacterial and protozoal A-sites reveal that fluorine acts only as an H-bond acceptor to favorably interact with G1408 of the protozoal A-site. Unlike aminoglycosides containing a 6'-amino group, 6'-F sisomicin cannot participate in the H-bonding pattern that characterizes stable pseudo base-pairs with A1408 of the bacterial A-sites. Based on these structural observations it may be possible to shift the biological activity of aminoglycosides to act preferentially as antiprotozoals. These findings expand the repertoire of small-molecules targeting the eukaryotic ribosome and demonstrate the usefulness of fluorine as a design element.
- Analysis of resistance genes of clinical Pannonibacter phragmitetus strain 31801 by complete genome sequencing. [Journal Article]
- AMArch Microbiol 2018 May 14
- To clarify the resistance mechanisms of Pannonibacter phragmitetus 31801, isolated from the blood of a liver abscess patient, at the genomic level, we performed whole genomic sequencing using a PacBi...
To clarify the resistance mechanisms of Pannonibacter phragmitetus 31801, isolated from the blood of a liver abscess patient, at the genomic level, we performed whole genomic sequencing using a PacBio RS II single-molecule real-time long-read sequencer. Bioinformatic analysis of the resulting sequence was then carried out to identify any possible resistance genes. Analyses included Basic Local Alignment Search Tool searches against the Antibiotic Resistance Genes Database, ResFinder analysis of the genome sequence, and Resistance Gene Identifier analysis within the Comprehensive Antibiotic Resistance Database. Prophages, clustered regularly interspaced short palindromic repeats (CRISPR), and other putative virulence factors were also identified using PHAST, CRISPRfinder, and the Virulence Factors Database, respectively. The circular chromosome and single plasmid of P. phragmitetus 31801 contained multiple antibiotic resistance genes, including those coding for three different types of β-lactamase [NPS β-lactamase (EC 126.96.36.199), β-lactamase class C, and a metal-dependent hydrolase of β-lactamase superfamily I]. In addition, genes coding for subunits of several multidrug-resistance efflux pumps were identified, including those targeting macrolides (adeJ, cmeB), tetracycline (acrB, adeAB), fluoroquinolones (acrF, ceoB), and aminoglycosides (acrD, amrB, ceoB, mexY, smeB). However, apart from the tripartite macrolide efflux pump macAB-tolC, the genome did not appear to contain the complete complement of subunit genes required for production of most of the major multidrug-resistance efflux pumps.
- Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR. [Journal Article]
- EIEnviron Int 2018 May 08; 117:146-153
- Wastewater treatment plants (WWTPs) contain diverse antibiotic resistance genes (ARGs), and thus are considered as a major pathway for the dissemination of these genes into the environments. However,...
Wastewater treatment plants (WWTPs) contain diverse antibiotic resistance genes (ARGs), and thus are considered as a major pathway for the dissemination of these genes into the environments. However, comprehensive evaluations of ARGs dynamic during wastewater treatment process lack extensive investigations on a broad spectrum of ARGs. Here, we investigated the dynamics of ARGs and bacterial community structures in 114 samples from eleven Chinese WWTPs using high-throughput quantitative PCR and 16S rRNA-based Illumina sequencing analysis. Significant shift of ARGs profiles was observed and wastewater treatment process could significantly reduce the abundance and diversity of ARGs, with the removal of ARGs concentration by 1-2 orders of magnitude. Whereas, a considerable number of ARGs were detected and enriched in effluents compared with influents. In particular, seven ARGs mainly conferring resistance to beta-lactams and aminoglycosides and three mobile genetic elements persisted in all WWTPs samples after wastewater treatment. ARGs profiles varied with wastewater treatment processes, seasons and regions. This study tracked the footprint of ARGs during wastewater treatment process, which would support the assessment on the spread of ARGs from WWTPs and provide data for identifying management options to improve ARG mitigation in WWTPs.
- The Use of Telemedicine for Penicillin Allergy Skin Testing. [Journal Article]
- JAJ Allergy Clin Immunol Pract 2018 May 08
- CONCLUSIONS: Telemedicine is an effective and novel approach to facilitate PST in the inpatient setting and carries a high degree of patient satisfaction. This method has the potential to optimize and improve access to Allergy/Immunology resources.
- Clustering Analysis of Antibiograms and Antibiogram Types of Streptococcus agalactiae Strains from Tilapia in China. [Journal Article]
- MDMicrob Drug Resist 2018 May 11
- In view of the changing antibiotic-resistance profiles of Streptococcus agalactiae from tilapia in China, antimicrobial susceptibilities of 75 S. agalactiae strains were determined by the disc diffus...
In view of the changing antibiotic-resistance profiles of Streptococcus agalactiae from tilapia in China, antimicrobial susceptibilities of 75 S. agalactiae strains were determined by the disc diffusion method, and cluster analyses of the antibiograms and antibiogram types were performed. All strains displayed multidrug resistance (MDR). The antimicrobial-resistance rates were highest (>90%) to aminoglycosides, sulfonamides, pipemidic acid, and norfloxacin, followed by penicillin, ampicillin, and ciprofloxacin (26.7-38.7%); those to furadantin, lincomycin, erythromycin, ofloxacin, tetracycline, and florfenicol were low (<10%), and no resistance to vancomycin, cefalexin, cefoxitin, amoxicillin, medemycin, doxitard, oxytetracycline, rifampin, chloramphenicol, or thiamphenicol was detected. Statistical analysis showed that the resistance rate to ciprofloxacin increased significantly in 2016 (p = 0.009), whereas that to trimethoprim/sulfamethoxazole decreased (p = 0.017). Cluster analyses identified that the strains had 23 antibiogram types (A-W) and clustered in five groups (Groups I-V). The strains with higher antimicrobial resistance mainly clustered in Groups I and II. Our results show that the antibiograms varied with time and by location and that antibiogram types are constantly updating and expanding. Effective measures must be taken to reduce the antimicrobial resistance and spread of MDR strains.
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- Defining the Relationship Between Phenotypic and Genotypic Resistance Profiles of Multidrug-Resistant Enterobacterial Clinical Isolates. [Journal Article]
- AEAdv Exp Med Biol 2018 May 11
- CONCLUSIONS: We suggest using blaTEM, blaCTX-M-15, qnr, and aac(6')-Ib-cr genes for better and faster prediction of suitable antibiotic therapy with effective doses against ESBL-producing isolates harboring plasmid-mediated quinolone resistance (PMQR) determinants. Amikacin, meropenem, gentamicin, and imipenem seem to be better choices of treatment for such life-threatening infections, because of their remaining highest activity.