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205,542 results
  • Concatenation of 14-3-3 with partner phosphoproteins as a tool to study their interaction. [Journal Article]
    Sci Rep 2019; 9(1):15007Tugaeva KV, Kalacheva DI, … Sluchanko NN
  • Regulatory 14-3-3 proteins interact with a plethora of phosphorylated partner proteins, however 14-3-3 complexes feature intrinsically disordered regions and often a transient type of interactions making structural studies difficult. Here we engineer and examine a chimera of human 14-3-3 tethered to a nearly complete partner HSPB6 which is phosphorylated by protein kinase A (PKA). HSPB6 includes …
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