- Biological assessment of ozone therapy on experimental oral candidiasis in immunosuppressed rats. [Journal Article]
- BBBiochem Biophys Rep 2018; 15:57-60
- Immunosuppressive drugs can compromise local or general defense mechanisms and can lead to oral candidiasis. Ozone therapy has a wide range of applications in almost every field of dentistry. Its uni…
Immunosuppressive drugs can compromise local or general defense mechanisms and can lead to oral candidiasis. Ozone therapy has a wide range of applications in almost every field of dentistry. Its unique properties include immunostimulant, analgesic, antihypnotic, detoxicating, and antimicrobial actions. Sixty male healthy rats were immunosuppressed with dexamethasone in their drinking water one week before candida infection. The animals were divided into four equal groups. Rats of group 1 were kept without any manipulation and those of group II were given oral inoculums of C. albicans on the dorsal surface of the tongue. Group III rats were handled as group II and instead the rats were treated by daily mycostatin drops local applicator as a routine treatment. Meanwhile, group IV rats were handled as group II and instead the rats were received daily intraperitoneal injection of 1 cm3 of ozone oxygen gas mixture with concentration of ozone 70 μg/cm3. After two weeks, all rats were euthanized and tongue specimens were prepared for histological staining with Haematoxylin & Eosin and CD3 immunohistochemical staining. Histological examination revealed that treatment with ozone therapy lead to gradual decrease in lingual papillary atrophy and invasion of candida yeast. Immunohistochemical study showed significant decrease in CD3 counting. We can conclude that ozone acts as an excellent fungicidal agent also, ozone is capable of alerting the immune system.
- Mildronate exerts acute anticonvulsant and antihypnotic effects. [Journal Article]
- BPBehav Pharmacol 2010; 21(5-6):548-55
- The effects of mildronate [3-(2,2,2-trimethylhydrazinium) propionate], an inhibitor of L-carnitine biosynthesis and an anti-ischaemic drug, were examined in various in-vivo conditions to investigate …
The effects of mildronate [3-(2,2,2-trimethylhydrazinium) propionate], an inhibitor of L-carnitine biosynthesis and an anti-ischaemic drug, were examined in various in-vivo conditions to investigate the neuropharmacological profile after acute administration. Mildronate (200 mg/kg, acute intraperitoneal administration) exerted anticonvulsant activity in a chemoconvulsant pentylenetetrazole-induced clonic and tonic seizure test but did not change the effects of a convulsion-inducing dose of (+)-bicuculline, a gamma-aminobutyric acid receptor antagonist. Mildronate also dose-dependently inhibited the sleeping time in ethanol-induced loss of righting reflex test. However, in a pentylenetetrazole-induced seizure test, mildronate significantly stimulated the anticonvulsant activity of ethanol. The anticonvulsant activity of mildronate was completely blocked after pre-treatment with alpha2-adrenergic receptor antagonist yohimbine (2 mg/kg) and nitric oxide synthase inhibitor N(G)-nitro-L-arginine (10 mg/kg). These results show that the acute administration of mildronate induces anticonvulsant and antihypnotic effects, which involve alpha2-adrenergic receptor and nitric oxide -dependent mechanisms. These findings indicate that the acute administration of mildronate could be beneficial for the treatment of seizures and alcohol intoxication.
- [Mechanism of drug dependency: answers to the questions of KATO]. [Journal Article]
- SSSeishin Shinkeigaku Zasshi 2000; 102(6):562-7
- The complicated mechanisms of drug dependency were discussed from the perspective of a reward, especially the perspective of psychic dependency of the brain. In Kato's article, the following question…
The complicated mechanisms of drug dependency were discussed from the perspective of a reward, especially the perspective of psychic dependency of the brain. In Kato's article, the following questions were raised. (1) Improper usage of terminology in describing the objective pharmacological behavior and describing the subjective psychic experience, e.g. the term "obsessional comfort". (2) The enhanced effects of morphine and d-amphetamine in ICSS (intracranial self-stimulation) were not suitably regarded, as these might be the results of stimulus current running in the brain. The conclusion that "morphine and d-amphetamine are 2 kinds of addictive drugs due to the reinforcement effects on ICSS" appears to be putting the incidental before the fundamental because these are addictive drugs in clinical use. (3) It was unknown why the basic problem of drug dependency was limited to psychic stimulant (antihypnotic, etc.), and why only opiates were represented as an addictive drug. (4) The conclusion that the reinforcement effects of barbital and benzodiazepine were not observed in ICSS contradicted Kato's conclusion, in which the reinforcement effects were detected in an experiment involving self-administration. (5) He reiterated that the usage of terminology was confused in describing the psychic experience and pharmacological behavioral. In response to the comments of the editors, I would answer the questions as follows: It is well-known that the terminologies used in psychiatry are strict and prudent. This should also be the case in the description of pharmacological behaviors in animal models. The author admitted Kato's profound relevant experiences in drug dependency, and did not disapprove of his criticisms about the terminology used in the article to describe objective behaviors and observe subjective experience. That article was excerpted from one of my lectures, which to some extent allowed freedom in phrases and sentences. The following are my answers to Kato's questions.
- Induction of methamphetamine-specific antibody using biodegradable carboxymethyl-chitin. [Journal Article]
- ABAnal Biochem 1987 Feb 15; 161(1):117-22
- Induction of a specific antibody for methamphetamine, an antihypnotic drug, has been studied using carboxymethyl-chitin (CM-chitin) as a hapten carrier. The hapten-specific antiserum was induced by o…
Induction of a specific antibody for methamphetamine, an antihypnotic drug, has been studied using carboxymethyl-chitin (CM-chitin) as a hapten carrier. The hapten-specific antiserum was induced by only a couple of hypodermal injections every 2 weeks. Inhibition of antigen-antibody complex formation was linearly related to methamphetamine concentration in the range 0.5 to 50 ng/50 microliter when an enzyme-linked immunosorbent assay was performed using an avidin-biotin-peroxidase complex. Little antibody directed against CM-chitin and CM-chitin oligomer was detected. Thus it seems to be advantageous in the hapten-bovine serum albumin system. The specificity of the antibody was high as shown by the use of various methamphetamine analogs.
- [Antisoporific and cardiotropic properties of new amino acid derivatives of nicotinic acid]. [Journal Article]
- FTFarmakol Toksikol 1986 May-Jun; 49(3):35-7
- On the models of urethane, nembutal and ethyl alcohol sleep it was shown that nicotinoylcapronate, nicotinoylserine and nicotinoylvaline possess antihypnotic properties being superior to those of cor…
On the models of urethane, nembutal and ethyl alcohol sleep it was shown that nicotinoylcapronate, nicotinoylserine and nicotinoylvaline possess antihypnotic properties being superior to those of cordiamine and on some models phenamine. Nicotinoylvaline and nicotinoylalanine normalized the myocardial contraction in animals with experimental ischemia.
- [Comparative study of the neurotropic and hormonal activity of thyrotropin releasing hormone and its analogs]. [Journal Article]
- PEProbl Endokrinol (Mosk) 1980 Mar-Apr; 26(2):48-52
- Comparative study of hormonal and antihypnotic activity of thyrotropine releasing hormone (TRH) and of its two analogues: pyroglutamine-serine-leucine amide (A-1) and pyroglutamine-serine-glycine ami…
Comparative study of hormonal and antihypnotic activity of thyrotropine releasing hormone (TRH) and of its two analogues: pyroglutamine-serine-leucine amide (A-1) and pyroglutamine-serine-glycine amide (A-2) was carried out on male rats. Along with hormonal activity, TRH possessed distinct antagonistic action against nembutal, diminishing its toxicity. In the group of control animals given nembutal and physiological saline the LD50 for nembutal constituted 5.75 mg/100 g. Administration of TRH in a dose of 0.5 mg/kg increased this index to 8.0 mg/100 g, and in a dose of 1 mg/kg--to 11 mg/100 g. The analogues under study failed to influence the TRH secretion of the hypophysis, but differed in respect to their action on nembutal: A-1 decreased nembutal toxicity more intensively than TRH, whereas A-2 was ineffective. The problem of the neutropic action of TRH and A-1 is discussed.
- [Relationship between the hypnotic action of medinal as well as the antihypnotic action of caffeine and the seasons]. [Journal Article]
- FTFarmakol Toksikol 1966 Sep-Oct; 29(5):540-2
- [Studies on glucuronic acid metabolism. 5. Antihypnotic effects of carbohydrates which have lactone rings on barbital action in rats]. [Journal Article]
- NYNihon Yakurigaku Zasshi 1962 Sep 20; 58:407-10