- Boosting mesenchymal stem cells regenerative activities on biopolymers-calcium phosphate functionalized collagen membrane. [Journal Article]
- CSColloids Surf B Biointerfaces 2019 Jun 15; 181:671-679
- The regeneration of bone-soft tissue interface, using functional membranes, remains challenging and can be promoted by improving mesenchymal stem cells (MSCs) paracrine function. Herein, a collagen m…
The regeneration of bone-soft tissue interface, using functional membranes, remains challenging and can be promoted by improving mesenchymal stem cells (MSCs) paracrine function. Herein, a collagen membrane, used as guided bone regeneration membrane, was functionalized by calcium phosphate, chitosan and hyaluronic acid hybrid coating by simultaneous spray of interacting species process. Composed of brushite, octacalcium phosphate and hydroxyapatite, the hybrid coating increased the membrane stiffness by 50%. After 7 days of MSCs culture on the hybrid coated polymeric membrane, biological studies were marked by a lack of osteoblastic commitment. However, MSCs showed an enhanced proliferation along with the secretion of cytokines and growth factors that could block bone resorption and favour endothelial cell recruitment without exacerbating polynuclear neutrophils infiltration. These data shed light on the great potential of inorganic/organic coated collagen membranes as an alternative bioactive factor-like platform to improve MSCs regenerative capacity, in particular to support bone tissue vascularization and to modulate inflammatory infiltrates.
- Draft Genome Sequence of Bacillus subtilis SB-14, an Antimicrobially Active Isolate from Namibian Social Spiders (Stegodyphus dumicola). [Journal Article]
- MRMicrobiol Resour Announc 2019 Jun 20; 8(25)
- We present the high-quality draft genome sequence of Bacillus subtilis SB-14, isolated from the Namibian social spider Stegodyphus dumicola In accordance with its antimicrobial activity, both known a…
We present the high-quality draft genome sequence of Bacillus subtilis SB-14, isolated from the Namibian social spider Stegodyphus dumicola In accordance with its antimicrobial activity, both known and potentially novel antimicrobial biosynthetic gene clusters were identified in the genome of SB-14.
- Cationic antimicrobial peptides and periodontal physiopathology: A systematic review. [Review]
- JPJ Periodontal Res 2019 Jun 19
- CONCLUSIONS: Given their role in innate immunity and their antimicrobial functions, LL-37 and α-defensins may be eligible as periodontal clinical biomarkers and could be an interesting way for therapeutic development.
- Lead and Other Trace Elements in Danish Birds of Prey. [Journal Article]
- AEArch Environ Contam Toxicol 2019 Jun 18
- Lead is a widely used and toxic heavy metal that poses a serious hazard to wildlife species and their ecosystems. Lead is used for production of hunting ammunition. Via gunshot or rifle projectiles, …
Lead is a widely used and toxic heavy metal that poses a serious hazard to wildlife species and their ecosystems. Lead is used for production of hunting ammunition. Via gunshot or rifle projectiles, it spreads in ecosystems and may end up in predators and scavengers feeding on wounded or dead animals shot with lead-based ammunition. To assess to what degree Danish raptors are subject to lead contamination, we measured the content of lead in liver tissue from Danish birds of prey (n = 137). Additionally, the study included values for 54 other trace elements. In our analysis, emphasis was put on interpretation of lead levels. Levels of cadmium, mercury and selenium were also discussed, while data for the remaining elements were provided for reference purposes. Bismuth was included to assess if lead originated from bismuth gunshot used as an alternative to lead shot. Concentrations of lead, cadmium, mercury and selenium were generally below the levels in similar studies of birds of prey in other northern European countries and none exceeded known and generally accepted threshold values for adverse health effects. As for lead, this is possibly related to the phase out of lead shot for hunting since 1986. The study confirms results from other studies showing that bismuth shot contains traces of lead that is deposited with bismuth in the target animal.
- Temporal trends of mercury differ across three northern white-tailed eagle (Haliaeetus albicilla) subpopulations. [Journal Article]
- STSci Total Environ 2019 Jun 04; 687:77-86
- The spatiotemporal trends of mercury (Hg) are crucial for the understanding of this ubiquitous and toxic contaminant. However, uncertainties often arise from comparison among studies using different …
The spatiotemporal trends of mercury (Hg) are crucial for the understanding of this ubiquitous and toxic contaminant. However, uncertainties often arise from comparison among studies using different species, analytical and statistical methods. The long-term temporal trends of Hg exposure were reconstructed for a key sentinel species, the white-tailed eagle (Haliaeetus albicilla). Body feathers were sampled from museum collections covering 150 years in time (from 1866 to 2015) from West Greenland (n = 124), Norway (n = 102), and Sweden (n = 87). A significant non-linear trend was observed in the Norwegian subpopulation, with a 60% increase in exposure occurring from 1866 to 1957 followed by a 40% decline until 2015. In the Swedish subpopulation, studied at a later period, the Hg exposure showed a drastic decline of 70% from 1967 to 2011. In contrast, no significant trend could be observed in the Greenland subpopulation. The additional analysis of dietary proxies (δ13C and δ15N) in general increased performance of the temporal trend models, but this was dependent on the subpopulation and study period. The downward trend of Hg coincided with the decreasing δ13C and δ15N in the Norwegian subpopulation, suggesting a potential dietary mitigation of Hg contamination. Hg exposure in both the Greenland and Norwegian subpopulations was consistently below the suggested threshold for adverse health effects (40.0 μg g-1), while the maximum exposure in the Swedish subpopulation was distinctively elevated (median: 46.0 μg g-1) and still remains well above natural background concentrations (maximum 5.0 μg g-1).
- Trophic Rewilding Advancement in Anthropogenically Impacted Landscapes (TRAAIL): A framework to link conventional conservation management and rewilding. [Journal Article]
- AAmbio 2019 Jun 14
- A variety of rewilding initiatives are being implemented across Europe, generally characterized by a more functionalist approach to nature management compared to the classic compositional approach. T…
A variety of rewilding initiatives are being implemented across Europe, generally characterized by a more functionalist approach to nature management compared to the classic compositional approach. To address the increasing need for a framework to support implementation of rewilding in practical management, we present TRAAIL-Trophic Rewilding Advancement in Anthropogenically Impacted Landscapes. TRAAIL has been co-produced with managers and other stakeholders and provides managers with a framework to categorize rewilding initiatives and to link conventional nature management and rewilding by guiding steps towards a higher degree of self-regulation. Applying TRAAIL to data obtained in a Danish survey of rewilding-inspired initiatives we find that out of 44 initiatives there is no "Full rewilding" initiatives, 3 "Near-full rewilding" initiatives, 23 "Partial rewilding" initiatives, 2 "minimal rewilding" initiatives and 16 "Effort-intensive conservation management" initiatives. This study shows how TRAAIL can guide and inform trophic rewilding on a local and national scale.
- Wafer-scale fabrication of high-quality tunable gold nanogap arrays for surface-enhanced Raman scattering. [Journal Article]
- NNanoscale 2019 Jun 13
- We report a robust and high-yield fabrication method for wafer-scale patterning of high-quality arrays of dense gold nanogaps, combining displacement Talbot lithography based shrink-etching with dry …
We report a robust and high-yield fabrication method for wafer-scale patterning of high-quality arrays of dense gold nanogaps, combining displacement Talbot lithography based shrink-etching with dry etching, wet etching, and thin film deposition techniques. By using the self-sharpening of <111>-oriented silicon crystal planes during the wet etching process, silicon structures with extremely smooth nanogaps are obtained. Subsequent conformal deposition of a silicon nitride layer and a gold layer results in dense arrays of narrow gold nanogaps. Using this method, we successfully fabricate high-quality Au nanogaps down to 10 nm over full wafer areas. Moreover, the gap spacing can be tuned by changing the thickness of deposited Au layers. Since the roughness of the template is minimized by the crystallographic etching of silicon, the roughness of the gold nanogaps depends almost exclusively on the roughness of the sputtered gold layers. Additionally, our fabricated Au nanogaps show a significant enhancement of surface-enhanced Raman scattering (SERS) signals of benzenethiol molecules chemisorbed on the structure surface, at an average enhancement factor up to 1.5 × 106.
- Postdeposition UV-Ozone Treatment: An Enabling Technique to Enhance the Direct Adhesion of Gold Thin Films to Oxidized Silicon. [Journal Article]
- ANACS Nano 2019 Jun 13
- We found that continuous films of gold (Au) on oxidized silicon (SiO2) substrates, upon treatment with ultraviolet (UV)-ozone, exhibit strong adhesion to the SiO2 support. Importantly, the enhancemen…
We found that continuous films of gold (Au) on oxidized silicon (SiO2) substrates, upon treatment with ultraviolet (UV)-ozone, exhibit strong adhesion to the SiO2 support. Importantly, the enhancement is independent of micro- or nanostructuring of such nanometer-thick films. Deposition of a second Au layer on top of the pretreated Au layer makes the adhesion stable for at least 5 months in environmental air. Using this treatment method enables us to large-scale fabricate various SiO2-supported Au structures at various thicknesses with dimensions spanning from a few hundreds of nanometers to a few micrometers, without the use of additional adhesion layers. We explain the observed adhesion improvement as polarization-induced increased strength of Auδ-Siδ+ bonds at the Au-SiO2 interface due to the formation of a gold oxide monolayer on the Au surface by the UV-ozone treatment. Our simple and enabling method thus provides opportunities for patterning Au micro/nanostructures on SiO2 substrates without an intermediate metallic adhesion layer, which is critical for biosensing and nanophotonic applications.
- Isolation and Immunocharacterization of Lactobacillus salivarius from the Intestine of Wakame-Fed Pigs to Develop Novel "Immunosynbiotics". [Journal Article]
- MMicroorganisms 2019 Jun 06; 7(6)
- Emerging threats of antimicrobial resistance necessitate the exploration of effective alternatives for healthy livestock growth strategies. 'Immunosynbiotics', a combination of immunoregulatory probi…
Emerging threats of antimicrobial resistance necessitate the exploration of effective alternatives for healthy livestock growth strategies. 'Immunosynbiotics', a combination of immunoregulatory probiotics and prebiotics with synergistic effects when used together in feed, would be one of the most promising candidates. Lactobacilli are normal residents of the gastrointestinal tract of pigs, and many of them are able to exert beneficial immunoregulatory properties. On the other hand, wakame (Undaria pinnafida), an edible seaweed, has the potential to be used as an immunoregulatory prebiotic when added to livestock feed. Therefore, in order to develop a novel immunosynbiotic, we isolated and characterized immunoregulatory lactobacilli with the ability to utilize wakame. Following a month-long in vivo wakame feeding trial in 8-week-old Landrace pigs (n = 6), sections of intestinal mucous membrane were processed for bacteriological culture and followed by identification of pure colonies by 16S rRNA sequence. Each isolate was characterized in vitro in terms of their ability to assimilate to the wakame and to differentially modulate the expression of interleukin-6 (IL-6) and interferon beta (IFN-β) in the porcine intestinal epithelial (PIE) cells triggered by Toll-like receptor (TLR)-4 and TLR-3 activation, respectively. We demonstrated that feeding wakame to pigs significantly increased the lactobacilli population in the small intestine. We established a wakame-component adjusted culture media that allowed the isolation and characterization of a total of 128 Lactobacilli salivarius colonies from the gut of wakame-fed pigs. Interestingly, several L. salivarius isolates showed both high wakame assimilation ability and immunomodulatory capacities. Among the wakame assimilating isolates, L. salivarius FFIG71 showed a significantly higher capacity to upregulate the IL-6 expression, and L. salivarius FFIG131 showed significantly higher capacity to upregulate the IFN-β expression; these could be used as immunobiotic strains in combination with wakame for the development of novel immunologically active feeds for pigs.
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- Association of dietary folate and vitamin B-12 intake with genome-wide DNA methylation in blood: a large-scale epigenome-wide association analysis in 5841 individuals. [Journal Article]
- AJAm J Clin Nutr 2019 Jun 05
- CONCLUSIONS: We identified novel epigenetic loci that are associated with folate and vitamin B-12 intake. Interestingly, we found a negative association between folate and DNA methylation. Replication of these methylation loci is necessary in future studies.