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5,601 results
  • Gastrulation in Drosophila melanogaster: Genetic control, cellular basis and biomechanics. [Review]
    Mech Dev. 2020 Jun 29 [Online ahead of print]Gheisari E, Aakhte M, Müller HJ
  • Gastrulation is generally understood as the morphogenetic processes that result in the spatial organization of the blastomere into the three germ layers, ectoderm, mesoderm and endoderm. This review summarizes our current knowledge of the morphogenetic mechanisms in Drosophila gastrulation. In addition to the events that drive mesoderm invagination and germband elongation, we pay particular atten…
  • A cell in hand is worth two in the embryo: recent advances in 2-cell like cell reprogramming. [Review]
    Curr Opin Genet Dev. 2020 Jun 26; 64:26-30.Iturbide A, Torres-Padilla ME
  • Multicellular organisms develop from a single cell, the zygote. This feature is referred to as totipotency. In the mouse, only the zygote and the 2-cell stage embryo display this attribute. Cells resembling the embryonic 2-cell stage blastomeres were identified in embryonic stem (ES) cell cultures as '2-cell-like cells' (2CLCs). This discovery brought the first cellular model with the possibility…
  • Pluripotency-associated genes reposition during early embryonic developmental stages in pigs. [Journal Article]
    Anim Sci J. 2020 Jan; 91(1):e13408.Nguyen HT, Nghia NT, … Kikuchi K
  • We examined the allelic expression and positioning of two pluripotency-associated genes, OCT4 and SOX2, and two housekeeping genes, ACTB and TUBA, in 4- and 8-cell porcine embryos utilizing RNA and DNA fluorescence in situ hybridization (FISH) in single blastomeres. The proportion of blastomeres expressing SOX2 bi-allelically increased from 45% at the 4-cell stage to 60% at the 8-cell stage. More…
  • Heterochromatin Morphodynamics in Late Oogenesis and Early Embryogenesis of Mammals. [Review]
    Cells. 2020 Jun 19; 9(6)Bogolyubova I, Bogolyubov D
  • During the period of oocyte growth, chromatin undergoes global rearrangements at both morphological and molecular levels. An intriguing feature of oogenesis in some mammalian species is the formation of a heterochromatin ring-shaped structure, called the karyosphere or surrounded "nucleolus", which is associated with the periphery of the nucleolus-like bodies (NLBs). Morphologically similar heter…
  • Application of platelet-rich plasma in the in vitro production of bovine embryos. [Journal Article]
    Trop Anim Health Prod. 2020 Jun 23 [Online ahead of print]Ramos-Deus P, Santos Nascimento P, … Oliveira MAL
  • The aim of this study was to replace fetal bovine serum (FBS) with platelet-rich plasma (PRP) for in vitro production of bovine embryos. The maturation media (TCM-199 medium) for the cumulus-oocyte complexes (COCs) was supplemented with 5% (G5) and 10% (G10) PRP or 10% FBS (GC). After fertilization, the presumed zygotes were randomly distributed in culture medium supplemented with 5% (G5) and 10%…
  • The role of RHOA signaling in trophectoderm cell-fate decision in cattle. [Journal Article]
    Biochem Biophys Res Commun. 2020 Jun 05 [Online ahead of print]Kohri N, Akizawa H, … Kawahara M
  • Mammalian blastocysts are composed of two distinct cell lineages, namely the inner cell mass (ICM) and trophectoderm (TE). TE cells that give rise to the embryonic placenta are marked by an exclusive expression of the key determinant transcription factor, CDX2. Although Hippo signaling pathway is known to be responsible for this TE-specific expression of CDX2, the upstream regulator of this pathw…
  • Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design. [Journal Article]
    Sci Adv. 2020 Apr; 6(17):eaay1751.Rosàs-Canyelles E, Modzelewski AJ, … Herr AE
  • The process by which a zygote develops from a single cell into a multicellular organism is poorly understood. Advances are hindered by detection specificity and sensitivity limitations of single-cell protein tools and by challenges in integrating multimodal data. We introduce an open microfluidic tool expressly designed for same-cell phenotypic, protein, and mRNA profiling. We examine difficult-t…
  • Sperm DNA damage causes genomic instability in early embryonic development. [Journal Article]
    Sci Adv. 2020 Apr; 6(16):eaaz7602.Middelkamp S, van Tol HTA, … Kuijk EW
  • Genomic instability is common in human embryos, but the underlying causes are largely unknown. Here, we examined the consequences of sperm DNA damage on the embryonic genome by single-cell whole-genome sequencing of individual blastomeres from bovine embryos produced with sperm damaged by γ-radiation. Sperm DNA damage primarily leads to fragmentation of the paternal chromosomes followed by random…
  • The impact of embryo binucleation depends upon its origin. [Journal Article]
    Reproduction. 2020 Jul; 160(1):V1-V4.Gomes Paim LM, FitzHarris G
  • Preimplantation embryos frequently contain binucleated cells, but reports differ as to whether binucleation affects development and whether such embryos should be used clinically. In this Point Of View article, we propose a possible explanation for this disparity: binucleation can arise by distinct routes, one that produces healthy blastomeres and one that directly threatens embryo viability.
  • Are polyploid giant cancer cells in high grade serous carcinoma of the ovary blastomere-like cancer stem cells? [Review]
    Ann Diagn Pathol. 2020 Mar 18; 46:151505.Salem A, Pinto K, … Silva EG
  • Polyploid giant cancer cells, either multinucleated or mononucleated, in high grade serous carcinoma of the ovary have been previously recognized. Different theories including degenerative changes or an important step in the development of high grade serous carcinoma have been proposed. Here we investigate possible explanations for the presence of polyploid giant cancer cells in high grade serous…
  • Spawning induction and embryonic development of Salminus hilarii (Characiformes: Characidae). [Journal Article]
    Zygote. 2020 May 08 [Online ahead of print]Araújo BC, Mello PH, … Honji RM
  • The present study aimed to evaluate two different methods of artificial reproduction induction, and characterize the embryonic development of Salminus hilarii. Different than observed for other tropical fish species, artificial reproduction induction followed by hand-stripping of gametes was considered unfeasible for S. hilarii, as no gamete fertilization was observed. However, females that were …
  • The ABCF gene family facilitates disaggregation during animal development. [Journal Article]
    Mol Biol Cell. 2020 Jun 15; 31(13):1324-1345.Skuodas S, Clemons A, … Fassler JS
  • Protein aggregation, once believed to be a harbinger and/or consequence of stress, age, and pathological conditions, is emerging as a novel concept in cellular regulation. Normal versus pathological aggregation may be distinguished by the capacity of cells to regulate the formation, modification, and dissolution of aggregates. We find that Caenorhabditis elegans aggregates are observed in large c…
  • Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development. [Journal Article]
    Sci Rep. 2020 Apr 10; 10(1):6255.Steele H, Makri D, … Kölle S
  • In artificial insemination the use of sex-sorted bovine sperm results in reduced conception, the causes of which are only partly understood. Therefore, we set out to investigate the effects of sexing on bovine sperm function and early embryonic development. Computer-assisted semen analysis (CASA) of sperm of the same bulls (n = 5), before and after sexing, demonstrated significantly reduced fast …
  • Molecular genetics of maternally-controlled cell divisions. [Journal Article]
    PLoS Genet. 2020 Apr; 16(4):e1008652.Abrams EW, Fuentes R, … Mullins MC
  • Forward genetic screens remain at the forefront of biology as an unbiased approach for discovering and elucidating gene function at the organismal and molecular level. Past mutagenesis screens targeting maternal-effect genes identified a broad spectrum of phenotypes ranging from defects in oocyte development to embryonic patterning. However, earlier vertebrate screens did not reach saturation, an…
  • CRISPR-on for activation of endogenous SMARCA4 and TFAP2C expression in bovine embryos. [Journal Article]
    Reproduction. 2020 Jun; 159(6):767-778.Savy V, Alberio V, … Salamone DF
  • CRISPR-mediated transcriptional activation, also known as CRISPR-on, has proven efficient for activation of individual or multiple endogenous gene expression in cultured cells from several species. However, the potential of CRISPR-on technology in preimplantation mammalian embryos remains to be explored. Here, we report for the first time the successful modulation of endogenous gene expression in…
  • Application of improved single blastomere fixation technique in preimplantation genetic diagnosis. [Journal Article]
    Cytotechnology. 2020 Apr; 72(2):217-226.Yu G, Ma S, … Hao A
  • To modify a fixation method improving the intensity and clarity of the single blastomeric signal detection by fluorescence in situ hybridization (FISH) in preimplantation genetic diagnosis. 333 cycles of assisted reproduction with preimplantation genetic diagnosis FISH (PGD-FISH) performed in our hospital were analyzed and a total of 3452 single blastomeres were obtained. For the conventional fix…
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