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217,175 results
  • CD4+CD25+ Regulatory T Cells Decreased CD8+IL-4+Cells in a Mouse Model of Allergic Asthma. [Journal Article]
    Iran J Allergy Asthma Immunol 2019; 18(4):369-378Li P, Li Z, … Chen J
  • Interleukin (IL)-4-producing-CD8 (cytotoxic T cells, Tc) contribute to lung eosinophilia and airway hyper-responsiveness (AHR) to an antigen. CD4+CD25+ regulatory T cells (Tregs) attenuate airway inflammation and AHR. This study investigated whether Tregs decrease Tc2frequencies in ovalbumin (OVA)-induced asthma model of mice. Female C57BL/6 mice were sensitized with OVA intraperitoneally and cha…
  • Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells. [Journal Article]
    Methods Mol Biol 2019; 2032:213-226Sen M, Wang L, … Carpenter EL
  • Noninvasive isolation of circulating tumor cells (CTCs) from patient blood samples allows for interrogation of valuable molecular and phenotypic information useful for disease diagnosis and monitoring response to therapy. However, CTCs are extremely rare relative to red and white blood cells (R/WBC), thus making CTC isolation from unmanipulated whole blood very time-consuming. Moreover, single CT…
  • Valid Post-clustering Differential Analysis for Single-Cell RNA-Seq. [Journal Article]
    Cell Syst 2019Zhang JM, Kamath GM, Tse DN
  • Single-cell computational pipelines involve two critical steps: organizing cells (clustering) and identifying the markers driving this organization (differential expression analysis). State-of-the-art pipelines perform differential analysis after clustering on the same dataset. We observe that because clustering "forces" separation, reusing the same dataset generates artificially low p values and…
  • Mechanisms of Interplay between Transcription Factors and the 3D Genome. [Review]
    Mol Cell 2019Kim S, Shendure J
  • Transcription factors (TFs) bind DNA in a sequence-specific manner and thereby serve as the protein anchors and determinants of 3D genome organization. Conversely, chromatin conformation shapes TF activity, for example, by looping TF-bound enhancers to distally located target genes. Despite considerable effort, our understanding of the mechanistic relation between TFs and 3D genome organization r…
  • An overview on enrichment methods for cell surface proteome profiling. [Review]
    J Sep Sci 2019Li Y, Qin H, Ye M
  • Cell surface proteins are essential for many important biological processes, including cell-cell interactions, signal transduction, and molecular transportation. With the characteristics of low abundance, high hydrophobicity, and high heterogeneity, it is difficult to get a comprehensive view of cell surface proteome by direct analysis. Thus, it is important to selectively enrich the cell surface…
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