- Umbilical cord-derived mesenchymal stromal/stem cells expressing IL-24 induce apoptosis in gliomas. [Journal Article]
- JCJ Cell Physiol 2019 Jul 12
- Although much progress has been made in the treatment of gliomas, the prognosis for patients with gliomas is still very poor. Stem cell-based therapies may be promising options for glioma treatment. …
Although much progress has been made in the treatment of gliomas, the prognosis for patients with gliomas is still very poor. Stem cell-based therapies may be promising options for glioma treatment. Recently, many studies have reported that umbilical cord-derived mesenchymal stromal/stem cells (UC-MSCs) are ideal gene vehicles for tumor gene therapy. Interleukin 24 (IL-24) is a pleiotropic immunoregulatory cytokine that has an apoptotic effect on many kinds of tumor cells and can inhibit the growth of tumors specifically without damaging normal cells. In this study, we investigated UC-MSCs as a vehicle for the targeted delivery of IL-24 to tumor sites. UC-MSCs were transduced with lentiviral vectors carrying green fluorescent protein (GFP) or IL-24 complementary DNA. The results indicated that UC-MSCs could selectively migrate to glioma cells in vitro and in vivo. Injection of IL-24-UC-MSCs significantly suppressed tumor growth of glioma xenografts. The restrictive efficacy of IL-24-UC-MSCs was associated with the inhibition of proliferation as well as the induction of apoptosis in tumor cells. These findings indicate that UC-MSC-based IL-24 gene therapy may be able to suppress the growth of glioma xenografts, thereby suggesting possible future therapeutic use in the treatment of gliomas.
- The synergy between radiographic and macroscopic observation of skeletal lesions on dry bone. [Journal Article]
- IJInt J Legal Med 2019 Jul 12
- The diagnosis of bone lesions is a fundamental part of the study of skeletal remains, both in the archeological and forensic context. On the one side, the literature proved the relevance of radiograp…
The diagnosis of bone lesions is a fundamental part of the study of skeletal remains, both in the archeological and forensic context. On the one side, the literature proved the relevance of radiography for the detection of bone lesions; on the other side, the careful macroscopic observation of the morphology of bone lesions is often underestimated. For this study, we examined and performed plain radiography on 14 skeletons of the CAL Milano Cemetery Skeletal Collection diagnosed with rheumatoid arthritis, diabetes, multiple myeloma, metastatic cancer, and osteomalacia to compare the macroscopic morphology and radiographic visualization of bone lesions. At least 200 osteolytic lesions and 65 areas of proliferative bone reaction (either spongiosclerotic or periosteal) were studied. We realized "comparative sets" of macroscopic pictures and radiographic imaging of the same skeletal elements to allow comparisons of detection and recognition of bone lesions. As a result, while trabecular lesions may be lost through naked eye observation, many lesions can also be unperceived on radiographs due to contrast, including periosteal reactions, osteolytic lesions, and spongiosclerosis. The aim of this research was to investigate the strengths and pitfalls of digital radiography and macroscopic analysis and to demonstrate the synergy of a complementary approach between the two methods for lesion analysis in dry bone.
- Exploring the role of music therapy in multiple sclerosis: brief updates from research to clinical practice. [Journal Article]
- NSNeurol Sci 2019 Jul 12
- CONCLUSIONS: We explored the efficacy of several MT programs, taking into account the different aspects of application feasibility in the clinical management of MS patients and the future challenges.
- Control of Synaptic Specificity by Establishing a Relative Preference for Synaptic Partners. [Journal Article]
- NNeuron 2019 Jun 28
- The ability of neurons to identify correct synaptic partners is fundamental to the proper assembly and function of neural circuits. Relative to other steps in circuit formation such as axon guidance,…
The ability of neurons to identify correct synaptic partners is fundamental to the proper assembly and function of neural circuits. Relative to other steps in circuit formation such as axon guidance, our knowledge of how synaptic partner selection is regulated is severely limited. Drosophila Dpr and DIP immunoglobulin superfamily (IgSF) cell-surface proteins bind heterophilically and are expressed in a complementary manner between synaptic partners in the visual system. Here, we show that in the lamina, DIP mis-expression is sufficient to promote synapse formation with Dpr-expressing neurons and that disrupting DIP function results in ectopic synapse formation. These findings indicate that DIP proteins promote synapses to form between specific cell types and that in their absence, neurons synapse with alternative partners. We propose that neurons have the capacity to synapse with a broad range of cell types and that synaptic specificity is achieved by establishing a preference for specific partners.
- Intercalated Cells of the Kidney Collecting Duct in Kidney Physiology. [Review]
- SNSemin Nephrol 2019; 39(4):353-367
- The epithelium of the kidney collecting duct (CD) is composed mainly of two different types of cells with distinct and complementary functions. CD principal cells traditionally have been considered t…
The epithelium of the kidney collecting duct (CD) is composed mainly of two different types of cells with distinct and complementary functions. CD principal cells traditionally have been considered to have a major role in Na+ and water regulation, while intercalated cells (ICs) were thought to largely modulate acid-base homeostasis. In recent years, our understanding of IC function has improved significantly owing to new research findings. Thus, we now have a new model for CD transport that integrates mechanisms of salt and water reabsorption, K+ homeostasis, and acid-base status between principal cells and ICs. There are three main types of ICs (type A, type B, and non-A, non-B), which first appear in the late distal convoluted tubule or in the connecting segment in a species-dependent manner. ICs can be detected in CD from cortex to the initial part of the inner medulla, although some transport proteins that are key components of ICs also are present in medullary CD, cells considered inner medullary. Of the three types of ICs, each has a distinct morphology and expresses different complements of membrane transport proteins that translate into very different functions in homeostasis and contributions to CD luminal pro-urine composition. This review includes recent discoveries in IC intracellular and paracrine signaling that contributes to acid-base regulation as well as Na+, Cl-, K+, and Ca2+ homeostasis. Thus, these new findings highlight the potential role of ICs as targets for potential hypertension treatments.
- The effects of prolonged working life on subjective quality of life across Europe. [Journal Article]
- SSSoc Sci Res 2019; 82:33-44
- This paper examines the effect of prolonged working careers on subjective quality of life (QoL) in four European regions. The paper tests a basic assumption of the role accumulation theory and the ac…
This paper examines the effect of prolonged working careers on subjective quality of life (QoL) in four European regions. The paper tests a basic assumption of the role accumulation theory and the active ageing approach that additional roles, including prolonged working careers, are beneficial for the quality of life of older people. The propensity score matching method was used on data from the Survey of Health, Ageing and Retirement in Europe (SHARE) for four European regions with distinctive economic, institutional, and cultural contexts connected to paid work. The context-sensitive effects of prolonged labour force participation on QoL as a whole, control, and pleasure are positive in regions in which financial need serves as a more important motivation to work than nonmaterial need. In contrast, the effects on QoL as a whole and autonomy are negative in wealthier and more developed European regions. The paper concludes that the main motivation for prolonged working careers seems to be to avoid deteriorating living standards; satisfactory retirement conditions should thus be an aim complementary to incentives for those who are willing and able to work longer.
- The role of health animators in malaria control: a qualitative study of the health animator (HA) approach within the Majete malaria project (MMP) in Chikwawa District, Malawi. [Journal Article]
- BHBMC Health Serv Res 2019 Jul 12; 19(1):478
- CONCLUSIONS: The HA approach is a useful strategy for complementing malaria prevention strategies in rural communities and improving practices for health-seeking behaviour. Various factors influence HAs' motivation, retention, community engagement, and programme sustainability. However, little is known about how these factors interact to influence volunteers' motivation, community participation and sustainability over time. More research is needed to explore the acceptability of an HA approach and the impact on malaria control in other rural communities in Malawi.
- Factors affecting tobacco smoking in Ethiopia: evidence from the demographic and health surveys. [Journal Article]
- BPBMC Public Health 2019 Jul 12; 19(1):938
- CONCLUSIONS: To effectively control tobacco smoking of the diverse communities in Ethiopia, policymakers should consider a multi-pronged policy approach that combines various policy tools that account for regional variation, the local social contexts, as well as the complementary nature of smoking and khat chewing practices.
- Use of nanotechnology for infectious disease diagnostics: application in drug resistant tuberculosis. [Journal Article]
- BIBMC Infect Dis 2019 Jul 12; 19(1):618
- CONCLUSIONS: The AuNP based lateral flow assay was capable of differentiating the specific mutation and the wild type along with MTb identification within 3 h.
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- Surgical Management of the Nose in Relation With the Fronto-Orbital Area to Change and Feminize the Eyes' Expression. [Journal Article]
- JCJ Craniofac Surg 2019; 30(5):1376-1379
- Gender affirmation surgeries in male-to-female transition patients include breast augmentation, genital construction, and facial feminization. Facial features serve an integral role in identifying an…
Gender affirmation surgeries in male-to-female transition patients include breast augmentation, genital construction, and facial feminization. Facial features serve an integral role in identifying an individual's gender, with certain characteristics perceived as feminine or masculine.The nose, as the centerpiece of the middle third of the face, is an important component of facial attractiveness and gender identity. Thus, reduction rhinoplasty is a mainstay of the facial feature remodeling (FFRS) and facial feminization surgery (FFS).The authors usually divide the surgery planning in 2 steps, obviously depending on each patient needs, but generally speaking, the authors do the upper part of the head in one stage and the mandible and face lift in another.If the patient only requires frontal bone surgery and softening of the orbital rims, the authors always perform the nose surgery in this stage. If a mandible reduction is needed, combined with a face-lift, the authors do it in another procedure, usually separated between 7 to 10 days from the other surgery. In these cases, the authors begin with the lower part of the face on the first stage and then, the frontal bone and rhinoplasty on the second.The fronto naso-orbital complex is one of the main facial areas that determine the identification of facial gender, the nose plays a crucial role in FFS of the middle third. The main goal of rhinoplasty in FFRS is to change masculine nasal features to feminine ones by performing dorsal reduction, tip refinement, and narrowing the nasal bones, most often using an external approach and cartilaginous grafts to provide good tip support and long-term stability. Lip-lift and frontonasal recontouring can be technically complementary to rhinoplasties associated with facial features remodeling and feminization.