- Barcoding of Chrysomelidae of Euro-Mediterranean area: efficiency and problematic species. [Journal Article]
- SRSci Rep 2018 Sep 07; 8(1):13398
- Leaf beetles (Coleoptera: Chrysomelidae), with more than 37,000 species worldwide and about 2,300 in the Euro-Mediterranean region, are an ecological and economical relevant family, making their mole…
Leaf beetles (Coleoptera: Chrysomelidae), with more than 37,000 species worldwide and about 2,300 in the Euro-Mediterranean region, are an ecological and economical relevant family, making their molecular identification of interest also in agriculture. This study, part of the Mediterranean Chrysomelidae Barcoding project (www.c-bar.org), aims to: (i) develop a reference Cytochrome c oxidase I (COI) library for the molecular identification of the Euro-Mediterranean Chrysomelidae; (ii) test the efficiency of DNA barcoding for leaf beetles identification; (iii) develop and compare optimal thresholds for distance-based identifications estimated at family and subfamily level, minimizing false positives and false negatives. Within this study, 889 COI nucleotide sequences of 261 species were provided; after the inclusion of information from other sources, a dataset of 7,237 sequences (542 species) was analysed. The average intra-interspecific distances were in the range of those recorded for Coleoptera: 1.6-24%. The estimated barcoding efficiency (~94%) confirmed the usefulness of this tool for Chrysomelidae identification. The few cases of failure were recorded for closely related species (e.g., Cryptocephalus marginellus superspecies, Cryptocephalus violaceus - Cryptocephalus duplicatus and some Altica species), even with morphologically different species sharing the same COI haplotype. Different optimal thresholds were achieved for the tested taxonomic levels, confirming that group-specific thresholds significantly improve molecular identifications.
- Updated checklist of Balearic leaf beetles (Coleoptera: Chrysomelidae). [Journal Article]
- ZZootaxa 2017 05 29; 4272(2):151-177
- The first updated checklist of Balearic leaf beetles (Chrysomelidae) since 1960 is presented here, evincing the presence of 118 species. This estimation is clearly lower than the 141 species reported…
The first updated checklist of Balearic leaf beetles (Chrysomelidae) since 1960 is presented here, evincing the presence of 118 species. This estimation is clearly lower than the 141 species reported in the only list available to date (Jolivet, 1953), and the dissimilarity is even more pronounced if we take into account that 22 new species have been added during this period. The possible explanations for these differences are discussed. The main island in the archipelago holds most of the species (Mallorca, 113 spp.), followed by Menorca (71 spp.), Eivissa (39 spp.) and Formentera (19 spp.). Thus, the Gymnesian islands (Mallorca and Menorca) are more species-rich than the Pityusic ones (Eivissa and Formentera). The number of species per island is significantly correlated with their respective areas not only for the Balearic but also for the much larger western Mediterranean islands of Corsica and Sardinia, and these abundances are not related with their nearness to the closest mainland. Among the different subfamilies and tribes, the Balearic flea-beetles (Alticinae) are clearly more prevalent whereas on the contrary, the Clytrini are less represented in comparison with the nearest mainland (Iberian Peninsula). The presented checklist includes four endemic species, Cryptocephalus majoricensis (Mallorca, Menorca and Formentera), C. tramuntanae (Mallorca), Cyrtonus majoricensis (Mallorca) and Timarcha balearica (Mallorca and Menorca). Furthermore, two adventitious species, Monoxia obesula and Epitrix hirtipennis of North American origin, have been reported for the first time in the Balearic Islands, in agreement with previous findings in other Mediterranean countries.
- Barcoding Chrysomelidae: a resource for taxonomy and biodiversity conservation in the Mediterranean Region. [Journal Article]
- ZZookeys 2016; (597):27-38
- The Mediterranean Region is one of the world's biodiversity hot-spots, which is also characterized by high level of endemism. Approximately 2100 species of leaf beetle (Coleoptera; Chrysomelidae) are…
The Mediterranean Region is one of the world's biodiversity hot-spots, which is also characterized by high level of endemism. Approximately 2100 species of leaf beetle (Coleoptera; Chrysomelidae) are known from this area, a number that increases year after year and represents 5/6% of the known species. These features, associated with the urgent need to develop a DNA-based species identification approach for a broad spectrum of leaf beetle species, prompted us to develop a database of nucleotide sequences, with a solid taxonomic background, for all the Chrysomelidae Latreille, 1802 sensu latu inhabiting the Mediterranean region. The Mediterranean Chrysomelidae Barcoding project, which has started in 2009, involves more than fifty entomologists and molecular biologists from different European countries. Numerous collecting campaigns have been organized during the first seven years of the project, which led to the collection of more than 5000 leaf beetle specimens. In addition, during these collecting campaigns two new allochthonous species for Europe, namely Ophraella communa LeSage, 1986 and Colasposoma dauricum Mannerheim, 1849, were intercepted and some species new to science were discovered (e.g., Pachybrachis sassii Montagna, 2011 and Pachybrachis holerorum Montagna et al., 2013). DNA was extracted from 1006 specimens (~13% of the species inhabiting the Mediterranean region) and a total of 910 cox1 gene sequences were obtained (PCR amplification efficiency of 93.8%). Here we report the list of the barcoded subfamilies, genera and the number of species for which cox1 gene sequences were obtained; the metadata associated with each specimen and a list of problematic species for which marker amplification failed. In addition, the nucleotide divergence within and between species and genera was estimated and values of intraspecific nucleotide divergence greater than the average have been discussed. Cryptocephalus quadripunctatus G. A. Olivier, 1808, Cryptocephalus rugicollis G. A. Olivier, 1791 and Exosoma lusitanicum Linnaeus, 1767) are representatives of these cases.
- Checklist of leaf beetles (Coleoptera: Chrysomelidae) from the state of Morelos, Mexico. [Journal Article]
- ZZootaxa 2016 Mar 07; 4088(1):91-111
- We record 116 genera and 366 species of Chrysomelidae from the state of Morelos, Mexico. This represents an increase of 9.3% in the species richness of these beetles for the state. Also, Morelos is c…
We record 116 genera and 366 species of Chrysomelidae from the state of Morelos, Mexico. This represents an increase of 9.3% in the species richness of these beetles for the state. Also, Morelos is currently the third most diverse state in leaf beetles within Mexico, with 16.78% of total species recorded for the country. The most diverse genera were Calligrapha, Disonycha, Blepharida, Leptinotarsa, Cryptocephalus, Systena, Alagoasa, Diabrotica and Pachybrachis, each with more than eight species. Most of these genera contain large, showy beetles. When the chrysomelid fauna is more fully understood, some of the genera of tiny beetles will likely prove to be more diverse.
- Taxonomic remarks, phylogeny and evolutionary notes on the leaf beetle species belonging to the Cryptocephalus sericeus complex (Coleoptera: Chrysomelidae: Cryptocephalinae). [Journal Article]
- ZZootaxa 2014 Aug 29; 3857(3):333-78
- A cladistic analysis was carried out for a complex of 33 species of the genus Cryptocephalus that has been recognized for some time as monophyletic. 55 morphological adult characters have been used, …
A cladistic analysis was carried out for a complex of 33 species of the genus Cryptocephalus that has been recognized for some time as monophyletic. 55 morphological adult characters have been used, with 119 character states. Analyses were performed using parsimony procedures as well as Bayesian inference. Further information about phylogenetic scenarios were obtained by combining the morphological dataset with a sequence of 507 bp of the central portion of the 18S ribosomal RNA. Illustrations are provided for most morphological characters used in the analyses. Besides, the following taxonomic changes are proposed here: subg. Cerodens has to be considered a new synonym of subg. Cryptocephalus; C. zambanellus is raised again to species level; C. sericeus intrusus has to be considered a new synonym of C. zambanellus; C. telueticus has to be considered a new synonym of C. azurescens; all the populations of C. violaceus ranging from Iberian Peninsula to central Alps are attributed to the subspecies scaffaiolus Burlini, formerly described from the Northern Apennines. Biogeographic remarks are given and some evolutionary hypotheses about the radiation of the monophylum are proposed on the basis of the acquired knowledge. An identification key for the species is also provided.
- Metamicrobiomics in herbivore beetles of the genus Cryptocephalus (Chrysomelidae): toward the understanding of ecological determinants in insect symbiosis. [Journal Article]
- ISInsect Sci 2015; 22(3):340-52
- The Cryptocephalus marginellus (Coleoptera: Chrysomelidae) complex is composed by six species that are supposed to have originated by events of allo- or parapatric speciation. In the present study we…
The Cryptocephalus marginellus (Coleoptera: Chrysomelidae) complex is composed by six species that are supposed to have originated by events of allo- or parapatric speciation. In the present study we investigated the alternative hypotheses that the bacterial communities associated with six populations of this species complex are shaped by environmental factors, or reflect the proposed pattern of speciation. The microbiota associated with the six populations, from five species of the complex, have been characterized through 16S rRNA pyrotag sequencing. Based on a 97% sequence similarity threshold, data were clustered into 381 OTUs, which were analyzed using a variety of diversity indices. The microbiota of C. acquitanus and C. marginellus (Calanques) were the most diverse (over 100 OTUs), while that from C. zoiai yielded less bacterial diversity (45 OTUs). Taxonomic assignment revealed Proteobacteria, Tenericutes and Firmicutes as the dominant components of these beetles' microbiota. The most abundant genera were Ralstonia, Sphingomonas, Rickettsia, and Pseudomonas. Different strains of Rickettsia were detected in C. eridani and C. renatae. The analysis of β-diversity revealed high OTU turnover among the populations of C. marginellus complex, with only few shared species. Hierarchical clustering taking into account relative abundances of OTUs does not match the phylogeny of the beetles, therefore we hypothesize that factors other than phylogenetic constraints play a role in shaping the insects' microbiota. Environmental factors that could potentially affect the composition of bacterial communities were tested by fitting them on the results of a multi-dimensional scaling analysis. No significant correlations were observed towards the geographic distances or the host plants, while the composition of the microbiota appeared associated with altitude. The metabolic profiles of the microbiotas associated with each population were inferred from bacterial taxonomy, and interestingly, the obtained clustering pattern was consistent with the host phylogeny.
- New Coleoptera records from New Brunswick, Canada: Megalopodidae and Chrysomelidae. [Journal Article]
- ZZookeys 2012; (179):321-48
- Zeugophora varians Crotch and the family Megalopodidae are newly recorded for New Brunswick, Canada. Twenty-eight species of Chrysomelidae are newly recorded for New Brunswick, including Acalymma gou…
Zeugophora varians Crotch and the family Megalopodidae are newly recorded for New Brunswick, Canada. Twenty-eight species of Chrysomelidae are newly recorded for New Brunswick, including Acalymma gouldi Barber, Altica knabii Blatchley, Altica rosae Woods, Altica woodsi Isely, Bassareus mammifer (Newman), Chrysolina marginata (Linnaeus), Chrysomela laurentia Brown, Crepidodera violacea Melsheimer, Cryptocephalus venustus Fabricius, Neohaemonia melsheimeri (Lacordaire), Neohaemonia nigricornis (Kirby), Pachybrachis bivittatus (Say), Pachybrachis m-nigrum (Melsheimer), Phyllobrotica limbata (Fabricius), Psylliodes affinis (Paykull), Odontota dorsalis (Thunberg), Ophraella communa (LeSage), Ophraella cribrata (LeConte), Ophraella notata (Fabricius), Systena hudsonias (Forster), Tricholochmaea ribicola (Brown), and Tricholochmaea rufosanguinea (Say), which are also newly recorded for the Maritime provinces. Collection data, habitat data, and distribution maps are presented for all these species.
- Prevalence and pathology of lungworm infection in bottlenose dolphins Tursiops truncatus from southwest Florida. [Journal Article]
- DADis Aquat Organ 2009 Dec 22; 88(1):85-90
- Parasitism of the respiratory system is a relatively common finding in stranded cetaceans; however, no systematic investigations regarding the severity, distribution, and clinical consequences of the…
Parasitism of the respiratory system is a relatively common finding in stranded cetaceans; however, no systematic investigations regarding the severity, distribution, and clinical consequences of these infections in bottlenose dolphins Tursiops truncatus have been conducted previously. The present study determined the prevalence of lungworm infections in dead stranded (n=22) and live bottlenose dolphins (n=44) from southwestern Florida, USA, during the period from 2003 to 2005. Dead stranded bottlenose dolphins were necropsied and lungs were examined visually, by palpation, and histologically for lesions consistent with verminous pneumonia. When present, nematodes were counted, measured, and identified to species based upon their morphology. Dolphin feces and blowhole swabs were collected and examined for nematode larvae. Lungworm prevalence was 77% in dead animals (n=22). The lesions in most cases were mild, chronic, and not the primary cause of death. Only 13% of dead animals examined had patent infections, with larvae present in blowhole and fecal cytology, and only 18% of animals had intact worms present at necropsy, with a geometric mean intensity of infection of 22.6 worms animal(-1). Intact worms were identified as either Halocercus lagenorhynchi or Skrjabinalius cryptocephalus. The highest prevalence of active infections was found in neonates and calves, including 1 stillborn calf. For free-ranging animals, all blowhole swabs (n=44) were negative, and fecal cytology (n=22) showed a 3% prevalence of patent infection. Findings from the present study support the theory that bottlenose dolphins can be infected transplacentally by lungworms. The impact that such infections may have on neonatal survival is unknown; however, these infections could increase neonatal mortality.