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43 results
  • Tension in haemoglobin revealed by Fe-His(F8) bond rupture in the fully liganded T-state. [Journal Article]
    J Mol Biol. 1997 Aug 15; 271(2):161-7.Paoli M, Dodson G, … Wilkinson AJ
  • In 1972, Perutz proposed that the low affinity of T-state haemoglobin is caused by tension in the bond between the iron and the proximal histidine, restraining the Fe from moving into the porphyrin plane on binding oxygen. This proposal has often been disputed. If such tension does exist, it will be manifest in the liganded T-state. Here we describe the structure of the fully liganded T-state cya…
  • Asymmetric hemoglobin hybrids. [Journal Article]
    J Mol Biol. 1996 Oct 18; 263(1):90-7.Marden MC, Griffon N, Poyart C
  • We have investigated the functional properties of hemoglobin (Hb) valency hybrids, and specifically whether it makes a difference if the oxidized subunits are on the same dimer (asymmetric hybrid) or not. CO recombination kinetics were used to probe the allosteric equilibrium of tetramers with two oxidized subunits. Asymmetric hybrids were prepared by mixing HbCO with a large excess of cyano-metH…
  • Oxidative conversion of thiocyanate to cyanide by oxyhemoglobin during acid denaturation. [Journal Article]
    Arch Biochem Biophys. 1995 Aug 01; 321(1):245-54.Seto Y
  • Human oxyhemoglobin (Hb-O2) converts thiocyanate to cyanide under acidic conditions, in a hemoglobin and thiocyanate concentration-dependent manner. Other naturally occurring hemoglobin derivatives and hematin did not result in cyanide-producing activity. Pre-denaturation completely destroyed cyanide production. In the presence of 10% phosphoric acid, 12 microM cyanide was produced by 1 mM Hb-O2 …
  • Effects of recombinant human hemoglobin on motor functions of the opossum esophagus. [Journal Article]
    J Pharmacol Exp Ther. 1995 May; 273(2):762-7.Conklin JL, Murray J, … Rosenthal G
  • Chemically altered hemoglobins are being investigated as blood substitutes. They may affect numerous biological processes since free hemoglobin binds nitric oxide (NO). Nitric oxide is a neural mediator of relaxation of the lower esophageal sphincter (LES) and esophageal peristalsis. We hypothesize that recombinant human hemoglobin (rHb1.1) alters esophageal motor function by scavenging NO. Contr…
  • Evaluation of the alkaline haematin D-575 method for haemoglobin estimation in east Africa. [Journal Article]
    Bull World Health Organ. 1994; 72(6):937-41.Lema OE, Carter JY, … Rees PH
  • In many health facilities in east Africa, haemoglobin estimation is performed using visual colour comparison methods. Efforts to establish colorimetric methods face numerous constraints, including the unavailability of standards for quality control. In contrast, the alkaline haematin D-575 method for haemoglobin estimation is a colorimetric method that uses primary standards prepared from pure, c…
  • [Clinical significance of free hemoglobin in blood serum in viral hepatitis]. [Journal Article]
    Klin Lab Diagn. 1993 May-JunLevina LD, Bezuglova IA, Bezuglov DA
  • Blood serum levels of extraerythrocytic hemoglobin (EEHb) were measured by the hemoglobin cyanide method in 71 patients with acute viral hepatitis B over the course of the disease. The mean values of this parameter in 30 normal subjects were found to be 0.67 +/- 0.03 g/l. In the patients in the acute period of hepatitis (weeks 1-2 of jaundice) EEHb levels were drastically elevated, particularly s…
  • Electron spin echo studies of hemoglobin cyanide and nitroxide derivatives. [Journal Article]
    Biochim Biophys Acta. 1987 Nov 05; 916(1):48-53.Krishna YV, Aruna B, Narayana PA
  • Electron spin echo envelope modulation studies are performed on human hemoglobin cyanide, hemoglobin nitroxide and hemoglobin nitroxide + inositol hexaphosphate at neutral pH. The modulation data are Fourier transformed and are analyzed in the frequency domain. The frequency components observed from hemoglobin cyanide indicate modulation from the coordinated nitrogen N-1 of the proximal imidazole…
  • Determination of haemoglobin in birds by a modified alkaline haematin (D-575) method. [Journal Article]
    Comp Biochem Physiol B. 1987; 86(4):701-4.O'Halloran J, Duggan PF, Myers AA
  • A recently published method for measuring human haemoglobin based on alkaline haematin (Zander et al., Clin. chem. Acta 136, 83-93, 1984) has been adopted for bird samples. The new method yields comparable haemoglobin values with that of a previously used alkaline haematin method. Levels of haemoglobin estimated using alkaline haematin were higher than for cyanhaemiglobin, the reference method fo…
  • Ethylene glycol-stabilized haemolysates as control material in haemoglobinometry. [Journal Article]
    Clin Chim Acta. 1983 Dec 15; 135(2):175-9.Franzini C
  • Human blood haemolysates containing ethylene glycol (final volume fraction 0.35) were prepared and stored at -20 degrees C (in the liquid state) up to 372 days. During the whole period, the total haemoglobin concentration (assayed in the material by means of the reference HiCN method) was found to be stable; spectral analysis also failed in detecting any deterioration, Hi formation being low. Goo…
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