- Molecular Characterization of 87 Functional Genes in Wheat Diversity Panel and Their Association With Phenotypes Under Well-Watered and Water-Limited Conditions. [Journal Article]
- FPFront Plant Sci 2019; 10:717
- Modern breeding imposed selection for improved productivity that largely influenced the frequency of superior alleles underpinning traits of breeding interest. Therefore, molecular diagnosis for the …
Modern breeding imposed selection for improved productivity that largely influenced the frequency of superior alleles underpinning traits of breeding interest. Therefore, molecular diagnosis for the allelic variations of such genes is important to manipulate beneficial alleles in wheat molecular breeding. We analyzed a diversity panel largely consisted of advanced lines derived from synthetic hexaploid wheats for allelic variation at 87 functional genes or loci of breeding importance using 124 high-throughput KASP markers. We also developed two KASP markers for water-soluble carbohydrate genes (TaSST-D1 and TaSST-A1) associated with plant height and thousand grain weight (TGW) in the diversity panel. KASP genotyping results indicated that beneficial alleles for genes underpinning flowering time (Ppd-D1 and Vrn-D3), thousand grain weight (TaCKX-D1, TaTGW6-A1, TaSus1-7B, and TaCwi-D1), water-soluble carbohydrates (TaSST-A1), yellow-pigment content (Psy-B1 and Zds-D1), and root lesion nematodes (Rlnn1) were fixed in diversity panel with frequency ranged from 96.4 to 100%. The association analysis of functional genes with agronomic and biochemical traits under well-watered (WW) and water-limited (WL) conditions revealed that 21 marker-trait associations (MTAs) were consistently detected in both moisture conditions. The major developmental genes such as Vrn-A1, Rht-D1, and Ppd-B1 had the confounding effect on several agronomic traits including plant height, grain size and weight, and grain yield in both WW and WL conditions. The accumulation of favorable alleles for grain size and weight genes additively enhanced grain weight in the diversity panel. Graphical genotyping approach was used to identify accessions with maximum number of favorable alleles, thus likely to have high breeding value. These results improved our knowledge on the selection of favorable and unfavorable alleles through unconscious selection breeding and identified the opportunities to deploy alleles with effects in wheat breeding.
- Composition of the holdfast polysaccharide from Caulobacter crescentus. [Journal Article]
- JBJ Bacteriol 2019 Jun 17
- Surface colonization is central to the lifestyles of many bacteria. Exploiting surface niches requires sophisticated systems for sensing and attaching to solid materials. Caulobacter crescentus synth…
Surface colonization is central to the lifestyles of many bacteria. Exploiting surface niches requires sophisticated systems for sensing and attaching to solid materials. Caulobacter crescentus synthesizes a polysaccharide-based adhesin known as the holdfast at one of its cell poles, which enables tight attachment to exogenous surfaces. The genes required for holdfast biosynthesis have been analyzed in detail, but difficulties in isolating analytical quantities of the adhesin have limited efforts to characterize its chemical structure. In this report, we describe a method to extract the holdfast from C. crescentus cultures and present a survey of its carbohydrate content. Glucose, 3-O-methylglucose, mannose, N-acetylglucosamine and xylose were detected in our extracts. Our results provide evidence that the holdfast contains a 1,4-linked backbone of glucose, mannose, N-acetylglucosamine and xylose that is decorated with branches at the C-6 positions of glucose and mannose. By defining the monosaccharide components in the polysaccharide, our work establishes a framework for characterizing enzymes in the holdfast pathway and provides a broader understanding of how polysaccharide adhesins are built.Importance: To colonize solid substrates, bacteria often deploy dedicated adhesins that facilitate attachment to surfaces. Caulobacter crescentus initiates surface colonization by secreting a carbohydrate-based adhesin called the holdfast. Because little is known about the chemical makeup of the holdfast, the pathway for its biosynthesis and the physical basis for its unique adhesive properties are poorly understood. This study outlines a method to extract the C. crescentus holdfast and describes the monosaccharide components contained within the adhesive matrix. The composition analysis adds to our understanding of the chemical basis for holdfast attachment and provides missing information needed to characterize enzymes in the biosynthetic pathway.
- Laboratory information management software for engineered mini-protein therapeutic workflow. [Journal Article]
- BBBMC Bioinformatics 2019 Jun 17; 20(1):343
- Protein based therapeutics are one of the fastest growing classes of novel medical interventions in areas such as cancer, infectious disease, and inflammation. Protein engineering plays an important …
Protein based therapeutics are one of the fastest growing classes of novel medical interventions in areas such as cancer, infectious disease, and inflammation. Protein engineering plays an important role in the optimization of desired therapeutic properties such as reducing immunogenicity, increasing stability for storage, increasing target specificity, etc. One category of protein therapeutics is nature-inspired bioengineered cystine-dense peptides (CDPs) for various biological targets. These engineered proteins are often further modified by synthetic chemistry. For example, candidate mini-proteins can be conjugated into active small molecule drugs. We refer to modified mini-proteins as "Optides" (Optimized peptides). To efficiently serve the multidisciplinary lab scientists with varied therapeutic portfolio research goals in a non-commercial setting, a cost effective extendable laboratory information management system (LIMS) is/was needed.
- Tunable gap plasmons in gold nanospheres adsorbed into a pH-responsive polymer film. [Journal Article]
- JCJ Colloid Interface Sci 2019 Jun 07; 553:197-209
- Plasmon nanorulers are exquisitely sensitive distance sensors that are based on the electromagnetic interaction between metal nanoparticles and surfaces. We hypothesize that nanorulers can act as qua…
Plasmon nanorulers are exquisitely sensitive distance sensors that are based on the electromagnetic interaction between metal nanoparticles and surfaces. We hypothesize that nanorulers can act as quantitative probes of processes such as particle aggregation and adsorption, and deploy them to investigate particle adsorption onto stimulus-responsive polymer films. While such systems have previously been qualitatively investigated with plasmon nanorulers, our quantitative analysis should provide deeper insights.
- The symbolic mediation of patient trust: Transnational health-seeking among Indian-Australians. [Journal Article]
- SSSoc Sci Med 2019 Jun 08; :112359
- This article explores whether this extension of treatment and medication possibilities, owing to greater transnational movement of goods and people, presents increasing challenges for maintaining pat…
This article explores whether this extension of treatment and medication possibilities, owing to greater transnational movement of goods and people, presents increasing challenges for maintaining patients' trust within nationally-bound healthcare systems, such as in Australia. We ran focus groups in Melbourne from June 2012 to June 2013 with 34 Indian migrants to Australia. Our findings illustrate discrepancies between the symbolic mediation of trust within Indian and Australian healthcare encounters. We find that Indian participants associated authoritative and decisive self-presentation by doctors with medical competence, conflicting with Western patient choice models which exalt patient autonomy and agency. We also find that trust in Indian healthcare encounters is mediated through the symbolic deployment of "strong medication" and the engendering of "hope" in patients. Australian doctors' failure to deploy these symbols in the same way contributes to distrust that Indian participants express towards Australian health professionals and healthcare systems. We conclude that in situations where patients have less familiarity with the symbolic repertoire shared by the majority of users of a national healthcare system, such as can be the case with recent migrants, difficulties and misunderstandings may arise in negotiating trust, providing a potential motivator for seeking alternatives transnationally.
- Increasing access to microfluidics for studying fungi and other branched biological structures. [Journal Article]
- FBFungal Biol Biotechnol 2019; 6:1
- CONCLUSIONS: This pre-packaging approach provides a simple, one step process to initiate microfluidics in any setting for fungal studies, bacteria-fungal interactions, and other biological inquiries. This process improves access to microfluidics for controlling biological microenvironments, and further enabling visual and quantitative analysis of fungal cultures.
- Simulation of Endovascular Aortic Repair Using 3D Printed Abdominal Aortic Aneurysm Model and Fluid Pump. [Journal Article]
- CICardiovasc Intervent Radiol 2019 Jun 13
- CONCLUSIONS: EVAR simulation was feasible and simulated all procedural steps with high fidelity. This model may be applicable for assessment of technical competencies and standard endovascular skill acquisition within vascular surgery training curricula.
- Factors influencing the practice of new graduate nurses: A focused ethnography of acute care settings. [Journal Article]
- JCJ Clin Nurs 2019 Jun 13
- CONCLUSIONS: One way to smooth the transition from academic to clinical settings for NGNs is by offering transition or orientation programmes that will provide them with stability and a reduced workload, allowing them to progressively deploy their competencies.Organizational and individual factors influence how new graduate nurses deploy their competencies. Clinical educators and nurse managers can help new nurses by acting on these factors. This study conforms to the COREQ Research Reporting Guidelines for qualitative studies. This article is protected by copyright. All rights reserved.
- The AMERIGO Lander and the Automatic Benthic Chamber (CBA): Two New Instruments to Measure Benthic Fluxes of Dissolved Chemical Species. [Journal Article]
- SSensors (Basel) 2019 Jun 10; 19(11)
- Marine environments are currently subject to strong ecological pressure due to local and global anthropic stressors, such as pollutants and atmospheric inputs, which also cause ocean acidification an…
Marine environments are currently subject to strong ecological pressure due to local and global anthropic stressors, such as pollutants and atmospheric inputs, which also cause ocean acidification and warming. These strains can result in biogeochemical cycle variations, environmental pollution, and changes in benthic-pelagic coupling processes. Two new devices, the Amerigo Lander and the Automatic Benthic Chamber (CBA), have been developed to measure the fluxes of dissolved chemical species between sediment and the water column, to assess the biogeochemical cycle and benthic-pelagic coupling alterations due to human activities. The Amerigo Lander can operate in shallow as well as deep water (up to 6000 m), whereas the CBA has been developed for the continental shelf (up to 200 m). The lander can also be used to deploy a range of instruments on the seafloor, to study the benthic ecosystems. The two devices have successfully been tested in a variety of research tasks and environmental impact assessments in shallow and deep waters. Their measured flux data show good agreement and are also consistent with previous data.
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- Ethical aspects of collateral clinical services by non-credentialled personnel in international medical crises. [Journal Article]
- IMIntern Med J 2019; 49(6):777-780
- The study of global responses to medical crises in developing and under-developed countries demands reflection about the ethical engagement of non-credentialled medical personnel in the context of cl…
The study of global responses to medical crises in developing and under-developed countries demands reflection about the ethical engagement of non-credentialled medical personnel in the context of clinical care. The need for, and the desire to contribute by, people and organisations that lack demonstrable or readily transferable credentialed skills must be reconciled with the ethical administration of collateral clinical services. The urgent need for the provision of such services must neither preclude their use nor permit their use irrespective of salient ethical considerations. One way to explore these issues is to examine a recent exemplar of a non-governmental organisation's utilisation of non-credentialled medical personnel during the Ebola outbreak in Sierra Leone. Although the group lacked clinical proficiency (apart from community health workers), it collaborated with experts in interment to train staff to bury those who had succumbed to the disease in respectful and hygienic individually marked graves. The group leveraged its cultural competency and in-country presence to deploy staff judiciously, support the workers to combat social ostracism and offer vocational and other education to help them assimilate back into the workforce once the outbreak subsided. The non-governmental organisation stepped up to do the work that others shunned, and to do it ethically and proficiently. I offer the work to show a compelling and innovative blueprint for ethical mobilisation of non-credentialed healthcare personnel in a global medical crisis.