Did you mean: (CIA )?
- OLFM4 expression in ductal carcinoma in situ and in invasive breast cancer cohorts by a SWATH-based proteomic approach. [Journal Article]
- PProteomics 2019 Jul 18; :e1800446
- Human Olfactomedin-4 (OLFM4) is a secreted protein involved in a variety of cellular functions including proliferation, differentiation, apoptosis and cell adhesion. OLFM4 expression has been studied…
Human Olfactomedin-4 (OLFM4) is a secreted protein involved in a variety of cellular functions including proliferation, differentiation, apoptosis and cell adhesion. OLFM4 expression has been studied in several tumor types including gastric, colorectal, lung and endometrioid cancers where it has been suggested to be an independant favorable or unfavorable prognostic marker. For breast cancer, the clinical significance of OLFM4 is still unclear. Proteomics analysis is a powerful approach to identify and quantify protein expression in tumor tissues. The data-independent acquisition (DIA) based approach of sequential window acquisition of all theoretical fragment-ion spectra (SWATH-MS) is a major advance in this field. In the present study, we used SWATH-MS as a tool for robust identification and quantification of breast tissue proteins. By employing a spectral library, we show that this strategy means we can quantify the OLFM4 in each sample together with a deep proteomic coverage which provides a more comprehensive idea of OLFM4 function in breast cancer. Our SWATH-MS data showed that OLFM4 expression was higher in DCIS than in invasive breast cancer. In-depth analysis of the breast tumor proteome showed that OLFM4 is a favorable pronostic marker. Serum OLFM4 levels in peripheral blood were also analyzed by ELISA in 825 cases, including 94 cases of healthy individuals, 61 cases of non invasive breast tumor (DCIS) and 670 cases of breast cancer (BC). We found that serum OLFM4 levels were significantly higher in the DCIS cohort and in the breast cancer cohort compared with the healthy controls. This result suggests that circulating OLFM4 could be an interesting biomarker of early breast cancer. Data are available via ProteomeXchange with identifier PXD014194. This article is protected by copyright. All rights reserved.
- Mitochondrial Respiration and H2O2 Emission in Saponin-permeabilized Murine Diaphragm Fibers: Optimization of Fiber Separation and Comparison to Limb Muscle. [Journal Article]
- AJAm J Physiol Cell Physiol 2019 Jul 17
- Diaphragm abnormalities in aging or chronic diseases include impaired mitochondrial respiration and H2O2 emission, which can be measured using saponin-permeabilized muscle fibers. Mouse diaphragm pre…
Diaphragm abnormalities in aging or chronic diseases include impaired mitochondrial respiration and H2O2 emission, which can be measured using saponin-permeabilized muscle fibers. Mouse diaphragm presents a challenge for isolation of fibers due to relatively high abundance of connective tissue in healthy muscle that is exacerbated in disease states. We tested a new approach to process mouse diaphragm for assessment of intact mitochondria respiration and ROS emission in saponin-permeabilized fibers. We used the red gastrocnemius (RG) as 'standard' limb muscle. Markers of mitochondrial content were 2-4 fold higher in diaphragm (Dia) than RG (p < 0.05). Maximal O2 consumption (JO2: pmol/s/mg) in Dia was higher with glutamate, malate, and succinate (Dia 399 ± 127, RG 148 ± 60; p < 0.05) and palmitoyl-CoA + carnitine (Dia 15 ± 5, RG 7 ± 1; p < 0.05) than RG, but not different between muscles when JO2 was normalized to citrate synthase activity. Absolute JO2 for Dia was 2-4 fold higher than reported in previous studies. Mitochondrial JH2O2 was higher in Dia than RG (p < 0.05), but lower in Dia than RG when JH2O2 was normalized to citrate synthase activity. Our findings are consistent with an optimized diaphragm preparation for assessment of intact mitochondria in permeabilized fiber bundles. The data also suggest that higher mitochondrial content potentially makes the diaphragm more susceptible to 'mitochondrial-onset' myopathy. Overall, the new approach will facilitate testing and understanding of diaphragm mitochondrial function in mouse models that are used to advance biomedical research and human health.
- Expert Group Recommendations on Detection and Management of Hypoglycemia in Routine Clinical Practice in Insulin Treated Patients with Diabetes. [Journal Article]
- JAJ Assoc Physicians India 2018; 66(12):90-97
- Hypoglycemia is a key barrier to optimum glycemic control in insulin treated diabetes patients. A national level expert group meeting was held at the 11th national insulin summit to analyze published…
Hypoglycemia is a key barrier to optimum glycemic control in insulin treated diabetes patients. A national level expert group meeting was held at the 11th national insulin summit to analyze published data from clinical studies and guidelines to evolve consensus recommendations on identification and management of hypoglycemia in insulin-treated diabetes patients. This consensus statement emphasizes consideration of suggestive symptoms or blood glucose levels ≤70 mg/dl and ability to self-treat in identification and classification of hypoglycemia. Patient questionnaire administration at each patient visit will enable accurate reporting of hypoglycemia. Patients with strict glycemic control, high glycemic variability, history of severe hypoglycemia, impaired hypoglycemia awareness, long duration of disease or insulin therapy could be at an increased risk of hypoglycemia. Prevention of hypoglycemia should include monitoring and goal setting, patient education, dietary intervention, exercise counseling and medication adjustment. Basal insulin analogues (vs. NPH), rapid-acting insulin analogues (vs. RHI) and premix insulin analogues (vs. BHI) are more appropriate options with superiority of insulin degludec to insulin glargine U100 and IDegAsp to BIAsp 30 to reduce the risk of hypoglycemia. This consensus statement provides practical guidance for physicians in effectively managing and minimizing the risk of hypoglycemia in insulin treated diabetes patients.
- Children's Comprehension of Irony: Studies on Polish-Speaking Preschoolers. [Journal Article]
- JPJ Psycholinguist Res 2019 Jul 16
- We explored the topic of irony comprehension by preschoolers. Two hundred and thirty-one children (77 four-year-olds, 89 five-year-olds, and 65 six-year-olds) were tested with the Irony Comprehension…
We explored the topic of irony comprehension by preschoolers. Two hundred and thirty-one children (77 four-year-olds, 89 five-year-olds, and 65 six-year-olds) were tested with the Irony Comprehension Task (ICT, Banasik and Bokus, in: Poster presented at the psycholinguistics conference in Flanders, Berg en Dal, 2012). Participants were asked questions checking comprehension of the intended meaning behind an ironic comment. Four conditions were used for the ironic utterances: targeted (ironic comment was a reference to the addressee's behavior), non-targeted (ironic comment was not a reference to the addressee's behavior), with symmetric dyads (a child said the ironic comment to another child), and asymmetric dyads (an adult said the ironic comment to a child). All groups achieved high irony comprehension scores. The results show a significant difference in accuracy between the 4-year-olds and the 6-year-olds only. The youngest group more accurately understood ironic utterances that referred to the addressee's action than those that did not, while older children did not show these differences. The aspect of who is speaking to whom was also significant only for the youngest children. These results provide important new insights into factors potentially influencing figurative language comprehension. Components such as participant structure and irony type require acknowledgement in the discussion on irony difficulty.
- Techniques for retrievability and for registering screw access holes in cement-retained implant-supported prostheses: A scoping review of the literature. [Review]
- JPJ Prosthet Dent 2019 Jul 12
- CONCLUSIONS: Cement-retained ISPs can be effectively retrieved by using interim cements and possibly by using a combined cement- and screw-retained design. Vacuum guides with guiding sleeves and computer-aided design and computer-aided manufacturing (CAD-CAM) guide templates are possible effective 3D techniques for registering the screw access channel (SAC) location and angulation.
- WinProphet: a user-friendly pipeline management system for proteomics data analysis based on Trans-Proteomic Pipeline. [Journal Article]
- ACAnal Chem 2019 Jul 15
- Protein and peptide identification and quantitation are essential tasks in proteomics research and involve a series of steps in analyzing mass spectrometry data. Trans-Proteomic Pipeline (TPP) provid…
Protein and peptide identification and quantitation are essential tasks in proteomics research and involve a series of steps in analyzing mass spectrometry data. Trans-Proteomic Pipeline (TPP) provides a wide range of useful tools through its web interfaces for analyses such as sequence database search, statistical validation, and quantitation. To utilize the powerful functionality of TPP without the need of manual intervention to launch each step, we develop a software tool, called WinProphet, to create and automatically execute a pipeline for proteomic analyses. It seamlessly integrates with TPP and other external command-line programs, supporting various functionalities including database search for protein and peptide identification, spectral library construction and search, DIA (Data-Independent Acquisition) data analysis, isobaric labeling and label-free quantitation. WinProphet is a standalone and installation-free tool with graphical interfaces for users to configure, manage, and automatically execute pipelines. The constructed pipelines can be exported as XML files with all of the parameter settings for reusability and portability. The executable files, user manual, and sample data sets of WinProphet are freely available at http://ms.iis.sinica.edu.tw/COmics/Software_WinProphet.html.
- Reliability and Accuracy of Clinical Assessment and Digital Image Analysis for Steatosis Evaluation in Discarded Human Livers. [Journal Article]
- TPTransplant Proc 2019 Jul 10
- CONCLUSIONS: Clinical assessment of steatosis is inaccurate, and relying on this method alone could result in unnecessary discard of livers. Digital image analysis is feasible with higher accuracy and reliability, but further clinical studies are required to evaluate its clinical validity.
- Peripheral nervous system alterations in infant and adult neurofibromatosis type 2. [Journal Article]
- NeurNeurology 2019 Jul 12
- CONCLUSIONS: Alterations of peripheral nerve segments occur early in the course of neurofibromatosis type 2 and are evident even in children not clinically affected by peripheral polyneuropathy. While those early alterations show similar characteristics compared to adults with neurofibromatosis type 2, the findings of this study suggest that secondary processes might be responsible for the development and severity of associated polyneuropathy.
- Investigation of serum proteome homeostasis during radiation therapy by a quantitative proteomics approach. [Journal Article]
- BRBiosci Rep 2019 07 12
- The purpose of this study is to analyse the serum proteome of patients receiving Radio Therapy (RT) at different stage of their treatment to discovery candidate biomarker of the radiation induced ski…
The purpose of this study is to analyse the serum proteome of patients receiving Radio Therapy (RT) at different stage of their treatment to discovery candidate biomarker of the radiation induced skin lesions and the molecular pathways underlying the radiation signatures. Six stages of RT treatment were monitored from patients treated because of brain cancer: before starting the treatment, during the treatment (four time points), and at 4 weeks from the last RT dose. Serum samples were analyzed by a proteomics approach based on the Data Independent Acquisition (DIA) mass spectrometry (MS). RT induced clear changes in the expression levels of 61 serum proteins. Among these, 26 proteins were down or up regulated significantly before the emergence of skin lesions and some of these were still deregulated after completion of the treatment. Few days before the appearance of the skin lesions, the levels of some proteins involved in the wound healing processes were downregulated. Pathway analysis indicated that after partial body irradiation the expression levels of proteins functionally involved in acute inflammatory and immune response, lipoprotein process and blood coagulation, were deregulated.
New Search Next
- Evolution from Covalent to Self-Assembled PAMAM-Based Dendrimers as Nanovectors for siRNA Delivery in Cancer by Coupled in Silico-Experimental Studies. Part II: Self-Assembled siRNA Nanocarriers. [Review]
- PPharmaceutics 2019 Jul 10; 11(7)
- In part I of this review, the authors showed how poly(amidoamine) (PAMAM)-based dendrimers can be considered as promising delivering platforms for siRNA therapeutics. This is by virtue of their preci…
In part I of this review, the authors showed how poly(amidoamine) (PAMAM)-based dendrimers can be considered as promising delivering platforms for siRNA therapeutics. This is by virtue of their precise and unique multivalent molecular architecture, characterized by uniform branching units and a plethora of surface groups amenable to effective siRNA binding and delivery to e.g., cancer cells. However, the successful clinical translation of dendrimer-based nanovectors requires considerable amounts of good manufacturing practice (GMP) compounds in order to conform to the guidelines recommended by the relevant authorizing agencies. Large-scale GMP-standard high-generation dendrimer production is technically very challenging. Therefore, in this second part of the review, the authors present the development of PAMAM-based amphiphilic dendrons, that are able to auto-organize themselves into nanosized micelles which ultimately outperform their covalent dendrimer counterparts in in vitro and in vivo gene silencing.