- Glycitin alleviates lipopolysaccharide-induced acute lung injury via inhibiting NF-κB and MAPKs pathway activation in mice. [Journal Article]
- IIInt Immunopharmacol 2019 Jul 12; 75:105749
- Acute lung injury (ALI) is a pulmonary diffuse dysfunction disease caused by immoderate inflammatory response breaking the coordination of physiological structures and functions, and there are very f…
Acute lung injury (ALI) is a pulmonary diffuse dysfunction disease caused by immoderate inflammatory response breaking the coordination of physiological structures and functions, and there are very few effective treatments to reduce high morbidity of ALI in critical patients. Glycitin is a natural ingredient derived from the seeds of leguminous plants and may have potent anti-inflammation features. The purpose of this study was to investigate the anti-inflammation effect of glycitin on LPS-induced ALI in mice and elucidate its possible anti-inflammatory mechanisms. The results of histopathological changes, the wet/dry weight ratio as well as the myeloperoxidase (MPO) activity indicated that glycitin obviously alleviated the lung injury induced by LPS. In addition, qPCR and ELISA results found that glycitin could dose-dependently decrease the expressions of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. Western blotting was performed to revealed that glycitin inhibited the activation of NF-κB and MAPKs signaling pathways by suppressing the expression of TLR4 protein and the phosphorylation of IKKβ, IκBα, p65, p38, ERK, and JNK. All data indicated that glycitin could protect lung tissues from LPS-induced inflammation via inhibiting TLR4-mediated NF-κB and MAPKs signaling pathways.
- Outcomes of Hematopoietic Cell Transplantation in Patients with Germline SAMD9/SAMD9L Mutations. [Journal Article]
- BBBiol Blood Marrow Transplant 2019 Jul 12
- Germline mutations in SAMD9 and SAMD9L genes cause MIRAGE (OMIM: *610456) and ataxia-pancytopenia (OMIM: *611170) syndromes, respectively, and are associated with chromosome 7 deletions, myelodysplas…
Germline mutations in SAMD9 and SAMD9L genes cause MIRAGE (OMIM: *610456) and ataxia-pancytopenia (OMIM: *611170) syndromes, respectively, and are associated with chromosome 7 deletions, myelodysplastic syndromes (MDS), and bone marrow failure. In this retrospective series, we report outcomes of allogeneic hematopoietic cell transplantation (HCT) in patients with hematologic disorders associated with SAMD9/SAMD9L mutations. Twelve patients underwent allogeneic HCT for MDS (n=10), congenital amegakaryocytic thrombocytopenia (n=1), and dyskeratosis congenita (n=1). Exome sequencing revealed heterozygous mutations in SAMD9 (n=6) or SAMD9L (n=6) genes. Four SAMD9 patients had features of MIRAGE syndrome. Median age at HCT was 2.8 years (range: 1.2-12.8). Conditioning was myeloablative in 9 cases and reduced-intensity in 3 cases. Syndrome-related comorbidities (diarrhea, infections, adrenal insufficiency, malnutrition, and electrolyte imbalance) were present in MIRAGE syndrome cases. One patient with a familial SAMD9L mutation, MDS and morbid obesity failed to engraft and died from refractory acute myeloid leukemia (AML). The other 11 patients achieved neutrophil engraftment. Acute post-transplant course was complicated by syndrome related comorbidities in MIRAGE cases. A patient with SAMD9L associated MDS, died of diffuse alveolar hemorrhage. The other 10 patients had resolution of hematological disorder and sustained peripheral blood donor chimerism. Ten out of 12 patients were alive with a median follow-up of 3.1 years (range: 0.1-14.7). More data are needed to refine transplant approaches in SAMD9/SAMD9L patients with significant comorbidities and to develop guidelines for their long-term follow-up.
- Structural and functional brain connectomes in patients with systemic lupus erythematosus. [Journal Article]
- EJEur J Neurol 2019 Jul 15
- CONCLUSIONS: Abnormalities of global and nodal structural connectivity occur in SLE-patients, especially with ADNA autoantibodies, with a diffuse disruption of structural integrity. Functional network integrity may contribute to preserve clinical functions. This article is protected by copyright. All rights reserved.
- Diffuse Infiltration of Multiple Myeloma With Initial Manifestation of Cavernous Sinus Syndrome Unveiled by 18F-FDG PET/CT. [Journal Article]
- CNClin Nucl Med 2019 Jul 12
- Intracranial infiltration is a rare complication of multiple myeloma (MM), which is usually seen in advanced stage. Here, we report a 47-year-old woman with only cavernous sinus syndrome as first man…
Intracranial infiltration is a rare complication of multiple myeloma (MM), which is usually seen in advanced stage. Here, we report a 47-year-old woman with only cavernous sinus syndrome as first manifestation. Brain MR revealed cavernous sinus masses, leptomeningeal nodules, and osseous erosion, which were considered metastases. F-FDG PET/CT, which was further performed to identify potential primary lesions, showed increased uptake not only in cavernous sinus and leptomeninges but also in diffuse bone lesions, hepatic nodules, lymph nodes, subcutaneous nodules, and bilateral ovarian masses. Multiple myeloma with intracranial involvement was confirmed by biopsy and cerebrospinal fluid examination.
- FDG-PET/CT Demonstrates Splenic Angiosarcoma Bone Marrow Metastasis. [Journal Article]
- CNClin Nucl Med 2019 Jul 12
- Some patients with splenic angiosarcoma initially manifest thrombocytopenia owing to hypersplenism or tumor-related bone marrow fibrosis. The diagnosis of bone marrow metastasis in patients with sple…
Some patients with splenic angiosarcoma initially manifest thrombocytopenia owing to hypersplenism or tumor-related bone marrow fibrosis. The diagnosis of bone marrow metastasis in patients with splenic angiosarcoma is challenging, as the presentation mimics idiopathic thrombocytopenic purpura or primary myelofibrosis. Our case showed diffuse FDG uptake in the vertebral bodies, sacroiliac bone, humerus, femur, and clavicles. It was pathologically diagnosed as bone marrow metastasis of splenic angiosarcoma. We believe that the heterogeneous FDG accumulation in the bone marrow, although nonspecific, can be useful for the diagnosis of the bone marrow metastasis in patients with splenic angiosarcoma.
- Incidental Finding of Acute Lymphocytic Leukemia in a Prostate-Specific Membrane Antigen PET/CT. [Journal Article]
- CNClin Nucl Med 2019 Jul 12
- PSMA PET/CT is known to show uptake in various benign and malignant processes. The following PSMA PET/CT was performed for prostate carcinoma staging (Gleason 3 + 4 left apex; PSA 5.8). It shows inci…
PSMA PET/CT is known to show uptake in various benign and malignant processes. The following PSMA PET/CT was performed for prostate carcinoma staging (Gleason 3 + 4 left apex; PSA 5.8). It shows incidental diffuse PSMA marrow uptake, not typical for prostate metastatic disease. No treatment had been commenced at the time of the scan. Serology and bone marrow biopsy showed B-cell acute lymphocytic leukemia. Focal PSMA uptake in the right ischium was correlated with a T1 hypointense lesion on a previous MRI and was convincing for a skeletal metastasis. Alternative diagnoses in diffuse skeletal PSMA uptake need therefore to be considered.
- Creutzfeldt-Jakob Disease Mimicking Neurosyphilis: A Complex Presentation, Histopathological Findings, and Special Precautions for Autopsy. [Journal Article]
- AJAm J Forensic Med Pathol 2019 Jul 12
- Creutzfeldt-Jakob disease (CJD) is a complex and rapidly fatal prion infection of the central nervous system with characteristic clinical and pathological findings. Herein, we present the case of an …
Creutzfeldt-Jakob disease (CJD) is a complex and rapidly fatal prion infection of the central nervous system with characteristic clinical and pathological findings. Herein, we present the case of an 80-year-old man with a 2-month history of rapid cognitive decline and ataxic gait. He was found to have a positive rapid plasma reagin and fluorescent treponemal antibody absorption (FTA-ABS) upon clinical testing and was presumed to have neurosyphilis. His neurological status precipitously declined during his hospitalization and he died. A complete autopsy was performed, which revealed diffuse spongiform change throughout the cerebrum. Brain tissue was sent to the National Prion Disease Surveillance Center, where immunostaining for prion protein (3F4) showed granular deposits, confirming the diagnosis of CJD. There have been rare cases reported in which CJD was clinically suspected but neurosyphilis was confirmed at autopsy. To our knowledge, this is the first case to be published in which the clinical findings strongly favored neurosyphilis, but spongiform encephalopathy was identified at autopsy. We review the clinical, radiographic, electrophysiological, laboratory, and histopathological features of both diseases and discuss the overlapping findings and inherent diagnostic difficulties. We also review the recommended protocols for safely handling suspected prion-infected autopsy tissue. A heightened awareness of the features of CJD and other prion diseases is needed among forensic pathologists, neuropathologists, and general autopsy pathologists to understand how to safely handle the tissue to get definite diagnoses for the decedent's family members and clinical care team.
- Infrared Nanospectroscopy at the Graphene-Electrolyte Interface. [Journal Article]
- NLNano Lett 2019 Jul 15
- We present a new methodology that enables studies of the molecular structure of graphene-liquid interfaces with nanoscale spatial resolution. It is based on Fourier transform infrared nanospectroscop…
We present a new methodology that enables studies of the molecular structure of graphene-liquid interfaces with nanoscale spatial resolution. It is based on Fourier transform infrared nanospectroscopy (nano-FTIR), where the infrared (IR) field is plasmonically enhanced near the tip apex of an atomic force microscope (AFM). The graphene seals a liquid electrolyte reservoir while acting also as a working electrode. The photon transparency of graphene enables IR spectroscopy studies of its interface with liquids, including water, propylene carbonate and aqueous ammonium sulfate electrolyte solutions. We illustrate the method by comparing IR spectra obtained by nano-FTIR and attenuated total reflection (which has a detection depth of a few microns) demonstrating that the nano-FTIR method makes possible to determine changes in speciation and ion concentration in the electric double and diffuse layers as a function of bias.
- Stability and Electronic Features of Calcium Hexa-, Hepta- and Octa-Coordinated Ammonia Complexes: A First Principles Study. [Journal Article]
- JPJ Phys Chem A 2019 Jul 15
- Neutral and positively charged calcium ammonia complexes are investigated by means of high-level quantum chemical calculations. We report optimal structures, binding energies and vibrational spectra …
Neutral and positively charged calcium ammonia complexes are investigated by means of high-level quantum chemical calculations. We report optimal structures, binding energies and vibrational spectra for Ca(NH3)1-80,+. The bigger Ca(NH3)6-80,+ complexes can be classified as solvated electron precursors (SEPs) and are best described as a Ca(NH3)6-82+ core with two or one peripheral electrons. In their ground state only ~10% of the outer electron density is estimated to be within the calcium van der Waals radius. For these systems we calculated several low-lying electronic states, where electrons populate diffuse outer orbitals. The Aufbau principle for the outer electrons is found identical to previously studied SEPs: 1s, 1p, 1d, 1f, 2s, 2p. We show that going from Ca(NH3)5, which has an incomplete first coordination shell and the two valence electrons are mainly in the valence sphere of calcium, to Ca(NH3)6 both the vibrational and electronic features change abruptly. Infrared, visible and ultra-violet spectroscopy can be used to identify and characterize calcium SEPs.
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- Nature of the 11Bu and 21Ag excited states of butadiene and the Goldilocks principle of basis set diffuseness. [Journal Article]
- JCJ Chem Theory Comput 2019 Jul 15
- Butadiene, being the simplest conjugated organic molecule, has been studied extensively by experiments and various high-level theoretical methods. Previous studies conclude that the complete active s…
Butadiene, being the simplest conjugated organic molecule, has been studied extensively by experiments and various high-level theoretical methods. Previous studies conclude that the complete active space (CAS) self-consistent field (SCF) method was unable to obtain the correct 11Bu and 21Ag state ordering and that it introduces artificial valence-Rydberg mixing into the 11Bu state because of the lack of external correlation. Basis sets and initial guesses specifically constructed for this problem were able to improve the vertical excitation energy of the 11Bu state, but did not resolve the controversy of the nature of the 11Bu state. In the present work, we demonstrate that, using standard intermediately-diffuse basis sets such as jul-cc-pVTZ and ma-TZVP, CASSCF is able to obtain the 11Bu and 21Ag states of trans-butadiene with much improved vertical excitation energy and reasonable wave function characteristics, and it provides a good reference wave function (capable of yielding quantitative excitation energies for excited states) for three methods that include external correlations in different ways, namely multiconfiguration pair-density functional theory (MC-PDFT), CAS second-order perturbation theory (PT2), and multistate (MS) CASPT2. We demonstrate that using combined analysis of the orbital second moments, state second moments, and MC-PDFT energy components is a very useful approach in determining excited-state characteristics and assigning states, and we show that basis sets without diffuse functions or with very diffuse basis functions are not stable or accurate in predicting the excited states of butadiene. We show the 21Ag state is valence-like and has an atypical double/single excitation character, and the 11Bu state has a certain degree of Rydberg character that is not artificial, settling the decades of controversy of the characters of these states.