- Development and Characterization of the Neuroregenerative Xanthohumol C/Hydroxypropyl-β-cyclodextrin Complex Suitable for Parenteral Administration. [Journal Article]Planta Med 2019PM
- The chroman-like chalcone Xanthohumol C, originally found in hops, was demonstrated to be a potent neuroregenerative and neuroprotective natural product and therefore constitutes a strong candidate for further pharmaceutical research. The bottleneck for in vivo experiments is the low water solubility of this chalcone. Consequently, we developed and validated a suitable formulation enabling in viv…
The chroman-like chalcone Xanthohumol C, originally found in hops, was demonstrated to be a potent neuroregenerative and neuroprotective natural product and therefore constitutes a strong candidate for further pharmaceutical research. The bottleneck for in vivo experiments is the low water solubility of this chalcone. Consequently, we developed and validated a suitable formulation enabling in vivo administration. Cyclodextrins were used as water-soluble and nontoxic complexing agents, and the complex of Xanthohumol C and 2-hydroxypropyl-β-cyclodextrin was characterized using HPLC, HPLC-MS, NMR, and differential scanning calorimetry. The water solubility of Xanthohumol C increases with increasing concentrations of cyclodextrin. Using 50 mM 2-hydroxypropyl-β-cyclodextrin, solubility was increased 650-fold. Furthermore, in vitro bioactivity of Xanthohumol C in free and complexed form did not significantly differ, suggesting the release of Xanthohumol C from 2-hydroxypropyl-β-cyclodextrin. Finally, a small-scaled in vivo experiment in a rat model showed that after i. p. administration of the complex, Xanthohumol C can be detected in serum, the brain, and the cerebrospinal fluid at 1 and 6 h post-administration. Mean (± SD) Xanthohumol C serum concentrations after 1, 6, and 12 h were determined as 463.5 (± 120.9), 61.9 (± 13.4), and 9.3 (± 0.8) ng/mL upon i. v., and 294.3 (± 22.4), 45.5 (± 0.7), and 13 (± 1.0) ng/mL after i. p. application, respectively. Accordingly, the formulation of Xanthohumol C/2-hydroxypropyl-β-cyclodextrin is suitable for further in vivo experiments and further pharmaceutical research aiming for the determination of its neuroregenerative potential in animal disease models.
- Goal identification before Spinal Cord Stimulation: a qualitative exploration in potential candidates. [Journal Article]Pain Pract 2019PP
- CONCLUSIONS: The interviews revealed a broad spectrum of individual patients' goals, highlighting the need of individually targeted rehabilitation trajectories in the field of neuromodulation. Goal identification could entail the first step towards individualized medicine in the SCS trajectory.
- Muscle-evoked Potentials After Electrical Stimulation to the Brain in Patients Undergoing Spinal Surgery are Less Affected by Anesthetic Fade With Constant-voltage Stimulation Than With Constant-current Stimulation. [Journal Article]Spine (Phila Pa 1976) 2019; 44(21):1492-1498S
- CONCLUSIONS: The CMAP waveform with constant-voltage stimulation is less susceptible to anesthetic fade than that with constant-current stimulation, even during long surgeries.
- Alterations of functional connectivity between thalamus and cortex before and after decompression in cervical spondylotic myelopathy patients: a resting-state functional MRI study. [Journal Article]Neuroreport 2019N
- CONCLUSIONS: Pre- or post-operative cervical spondylotic myelopathy patients showed functional connectivity alteration between thalamus and cortex, which suggest adaptive changes may favor the preservation of cortical sensorimotor networks before and after cervical cord decompression, and supply the improvement of clinical function.
- Do the Benefits of Routine Perioperative Chemoprophylaxis for Prevention of Thrombotic Events in Multilevel Posterior Surgery of the Cervical or Thoracic Spinal Cord Outweigh the Risks? [Journal Article]Clin Spine Surg 2019CS
- Mechanisms of improved exercise capacity following respiratory muscle training in athletes with cervical spinal cord injury. [Journal Article]J Physiol 2019JP
- Protective Effect of Contralateral, Ipsilateral, and Bilateral Remote Ischemic Preconditioning on Spinal Cord Ischemia Reperfusion Injury in Rats. [Journal Article]Turk Neurosurg 2019TN
- CONCLUSIONS: The results of this study show that contralateral, ipsilateral, and bilateral limb RIPC may reduce the complications of spinal cord ischemic injury.
- FAIR SCI Ahead: the evolution of the Open Data Commons for preclinical spinal cord injury research (ODC-SCI.org). [Journal Article]J Neurotrauma 2019JN
- Over the last 5 years multiple stakeholders in the field of spinal cord injury (SCI) research have initiated efforts to promote publications standards and to enable sharing of experimental data. In 2016 NIH/NINDS hosted representatives from the SCI community to streamline these efforts and to discuss the future of data sharing in the field according to the FAIR (Findable, Accessible, Interoperabl…
Over the last 5 years multiple stakeholders in the field of spinal cord injury (SCI) research have initiated efforts to promote publications standards and to enable sharing of experimental data. In 2016 NIH/NINDS hosted representatives from the SCI community to streamline these efforts and to discuss the future of data sharing in the field according to the FAIR (Findable, Accessible, Interoperable and Reusable) data stewardship principles1. As a next step, a multi-stakeholder group hosted a 2017 symposium in Washington D.C. entitled "FAIR SCI Ahead: the Evolution of the Open Data Commons for Spinal Cord Injury research". The goal of this meeting was to receive feedback from the community regarding infrastructure, policies and organization of a community-governed Open Data Commons (ODC) for preclinical SCI research. Here we summarize the policy outcomes of this meeting and report on progress implementing these policies in the form of a digital ecosystem: the Open Data Commons for Spinal Cord Injury (ODC-SCI.org). ODC-SCI enables data management, harmonization and controlled sharing of data in a manner consistent with the well-established norms of scholarly publication. Specifically, ODC-SCI is organized around virtual 'laboratories' with the ability to share data within each of 3 distinct data sharing spaces: within the laboratory, across verified laboratories, or publicly under a creative commons license (CC-BY 4.0) with digital object identifier (DOI) that enables data citation. The ODC-SCI implements FAIR data sharing and enables pooled data-driven discovery while crediting the generators of valuable SCI data.
- Preliminary evidence-based recommendations for return to learn: a novel pilot study tracking concussed college students. [Journal Article]Concussion 2019; 4(2):CNC63C
- CONCLUSIONS: We introduce a novel approach to monitor concussed students throughout their recovery, as well as factors that may influence concussion recovery process.
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- Adult Goat Retinal Neuronal Culture: Applications in Modeling Hyperglycemia. [Journal Article]Front Neurosci 2019; 13:983FN
- Culture of adult neurons of the central nervous system (CNS) can provide a unique model system to explore neurodegenerative diseases. The CNS includes neurons and glia of the brain, spinal cord and retina. Neurons in the retina have the advantage of being the most accessible cells of the CNS, and can serve as a reliable mirror to the brain. Typically, primary cultures utilize fetal rodent neurons…
Culture of adult neurons of the central nervous system (CNS) can provide a unique model system to explore neurodegenerative diseases. The CNS includes neurons and glia of the brain, spinal cord and retina. Neurons in the retina have the advantage of being the most accessible cells of the CNS, and can serve as a reliable mirror to the brain. Typically, primary cultures utilize fetal rodent neurons, but very rarely adult neurons from larger mammals. Here, we cultured primary retinal neurons isolated from adult goat up to 10 days, and established an in vitro model of hyperglycemia for performing morphological and molecular characterization studies. Immunofluorescence staining revealed that approximately 30-40% of cultured cells expressed neuronal markers. Next, we examined the relative expression of cell adhesion molecules (CAMs) in adult goat brain and retina. We also studied the effect of different glucose concentrations and media composition on the growth and expression of CAMs in cultured retinal neurons. Hyperglycemia significantly enhances neurite outgrowth in adult retinal neurons in culture. Expression of CAMs such as Caspr1, Contactin1 and Prion is downregulated in the presence of high glucose. Hyperglycemia downregulates the expression of the transcription factor CCAAT/enhancer binding protein (C/EBP α), predicted to bind CAM gene promoters. Collectively, our study demonstrates that metabolic environment markedly affects transcriptional regulation of CAMs in adult retinal neurons in culture. The effect of hyperglycemia on CAM interactions, as well as related changes in intracellular signaling pathways in adult retinal neurons warrants further investigation.