- Growth of Salmonella and Other Foodborne Pathogens on Inoculated Inshell Pistachios during Simulated Delays between Hulling and Drying. [Journal Article]
- JFJ Food Prot 2019; 82(5):815-825
- During harvest, pistachios are hulled, separated in water into floater and sinker streams (in large part on the basis of nut density), and then dried before storage. Higher prevalence and levels of S…
During harvest, pistachios are hulled, separated in water into floater and sinker streams (in large part on the basis of nut density), and then dried before storage. Higher prevalence and levels of Salmonella were previously observed in floater pistachios, but contributing factors are unclear. To examine the behavior of pathogens on hulled pistachios during simulated drying delays, floater and sinker pistachios collected from commercial processors were inoculated at 1 or 3 log CFU/g with cocktails of Salmonella and in some cases Escherichia coli O157:H7 or Listeria monocytogenes and incubated for up to 30 h at 37°C and 90% relative humidity. Populations were measured by plating onto tryptic soy agar and appropriate selective agars. In most cases, no significant growth (P > 0.05) of Salmonella was observed in the first 3 h after inoculation in hulled floaters and sinkers. Growth of Salmonella was greater on floater pistachios than on corresponding sinkers and on floater pistachios with ≥25% hull adhering to the shell surface than on corresponding floaters with <25% adhering hull. Maximum Salmonella populations (2 to 7 log CFU/g) were ∼2-log higher on floaters than on corresponding sinkers. The growth of E. coli O157:H7 and Salmonella on hulled pistachios was similar, but a longer lag time (approximately 11 h) and significantly lower maximum populations (4 versus 5 to 6 log CFU/g; P < 0.05) were predicted for L. monocytogenes. Significant growth of pathogens on hulled pistachios is possible when delays between hulling and drying are longer than 3 h, and pathogen growth is enhanced in the presence of adhering hull material.
- Age-Related Differences in the Physical and Physiological Demands during Small-Sided Games with Floaters. [Journal Article]
- SSports (Basel) 2019 Apr 02; 7(4)
- The purpose of this study was to compare the physical and physiological demands of a small-sided game (SSG) in three different age groups (senior, under-19 [U-19] and under-17 [U-17]) belonging to th…
The purpose of this study was to compare the physical and physiological demands of a small-sided game (SSG) in three different age groups (senior, under-19 [U-19] and under-17 [U-17]) belonging to the same academy. A further aim was to contrast the physical and physiological profiles of normal and floater players during this task. Thirty male football players performed a 4 vs. 4 + 2 floaters on a playing field of 40 by 30m for four bouts of 4 min with 2 min of passive recovery. In addition to heart rate (mean and maximal), a GPS (Global Positioning System) system was used to record the distances covered at different speeds, the number of accelerations and decelerations, and the work/rest ratio (W:R Ratio). Analysis of the data showed that the demands of the SSGs are determined by the age of the players and that the regular players have greater demands than floater players in the SSGs utilized. These results suggest that the coaches should pay attention to the promotion of players to superior teams because there are physical differences between them (especially the U-17 to U-19 teams). Likewise, coaches should understand that floaters are a useful tool for regulating the training load of players and programming the return-to-play process, as floater players experience lower demands than normal players.
- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration. [Journal Article]
- MMMethods Mol Biol 2019; 1953:151-162
- The cross talk between tumor cells and other cells present in the tumor microenvironment such as stromal and immune cells highly influences the behavior and progression of disease. Understanding the …
The cross talk between tumor cells and other cells present in the tumor microenvironment such as stromal and immune cells highly influences the behavior and progression of disease. Understanding the underlying mechanisms of interaction is a prerequisite to develop new treatment strategies and to prevent or at least reduce therapy failure in the future. Specific reactivation of the patient's immune system is one of the major goals today. However, standard two-dimensional (2D) cell culture techniques lack the necessary complexity to address related questions. Novel three-dimensional (3D) in vitro models-embedded in a matrix or encapsulated in alginate-recapitulate the in vivo situation much better. Cross talk between different cell types can be studied starting from co-cultures. As cancer immune modulation is becoming a major research topic, 3D in vitro models represent an important tool to address immune regulatory/modulatory questions for T, NK, and other cells of the immune system. The 3D systems consisting of tumor cells, fibroblasts, and immune cells (3D-3) already proved as a reliable tool for us. For instance, we made use of those models to study the molecular mechanisms of the cross talk of non-small cell lung cancer (NSCLC) and fibroblasts, to unveil macrophage plasticity in the tumor microenvironment and to mirror drug responses in vivo. Generation of those 3D models and how to use them to study immune cell infiltration and activation will be described in the present book chapter.
- Nd:YAG Laser Vitreolysis for Symptomatic Vitreous Floaters: Application of Infrared Fundus Photography in Assessing the Treatment Efficacy. [Journal Article]
- JOJ Ophthalmol 2019; 2019:8956952
- CONCLUSIONS: Nd:YAG vitreolysis was effective and safe in alleviating the visual symptoms induced by floaters. Quantification of floater shadow areas on infrared fundus photography could serve as an objective index for assessing treatment efficacy of Nd:YAG vitreolysis.
- Unilateral endogenous Candida endophthalmitis after extracorporeal shock wave lithotripsy for a renal stone in an immunocompetent woman: a case report. [Journal Article]
- JOJ Ophthalmic Inflamm Infect 2019 Feb 20; 9(1):5
- CONCLUSIONS: Endogenous Candida endophthalmitis following renal stone lithotripsy is a serious and rare intraocular infection that may happen in healthy individuals without any risk factors.
- [Multiple Brain Embolism Due to Brachiocephalic Artery:A Case Report]. [Journal Article]
- NSNo Shinkei Geka 2019; 47(1):91-96
- Embolisms arising from the brachiocephalic artery are very rare, of which there are few reports. We treated a patient with cerebral embolism originating from the brachiocephalic artery. The patient w…
Embolisms arising from the brachiocephalic artery are very rare, of which there are few reports. We treated a patient with cerebral embolism originating from the brachiocephalic artery. The patient was a 71-year-old man with high blood pressure, diabetes, and hyperlipidemia, who presented with a sudden disturbance of consciousness and left hemiparesis, and cerebral infarction of the right frontal lobe, right parietal lobe, bilateral occipital lobe, and bilateral hemisphere of cerebellum. There was no significant stenosis of a major artery or atrial fibrillation. A floater in the blood vessel from the calcified part of the origin of the brachiocephalic artery was confirmed and assessed to be an occurrence due to cerebral embolism of the right internal carotid artery and basilar artery domains. Anticoagulant medical treatment was continued and the floating thrombus disappeared three months after onset. It was thought that it originated from the brachiocephalic artery, due to an embolism with clot adhesion. When treating a patient with a cerebral embolism that does not accord with the vascular territory, it was thought that elucidating the etiology using various modalities is important.
- Coping with intrasexual behavioral differences: Capture-recapture abundance estimation of male cheetah. [Journal Article]
- EEEcol Evol 2018; 8(18):9171-9180
- Population estimates are a fundamental requirement of ecology and conservation. While capture-recapture models are an established method for producing such estimates, their assumption of homogeneous …
Population estimates are a fundamental requirement of ecology and conservation. While capture-recapture models are an established method for producing such estimates, their assumption of homogeneous capture probabilities is problematic given that heterogeneity in individual capture probability is inherent to most species. Such variation must be accounted for by abundance models; otherwise, biased estimates are risked.Here, we investigate the performance of four types of heterogeneity models for estimating abundance of male cheetah Acinonyx jubatus, a species with two distinct spatial tactics of territorial and nonterritorial (floater) males. The differences in spatial movements of territory holders and floaters are expected to result in intrasexual heterogeneous capture probabilities. Four heterogeneity models were used to model male abundance at five territories in central Namibia; (a) a spatial tactic model, (b) a finite mixture model, both run in program MARK, (c) a floater-only model, and (d) a heterogeneity Mh model, both run in the program CAPTURE. Camera trap data of cheetah, taken at frequently visited marking trees, were used to derive true abundance. Model results were compared to the true abundance to assess the accuracy of estimates.Only models (a), (b), and (c) were able to consistently produce accurate results. Mixture models do not require prior knowledge regarding spatial tactic of males, which might not always be available. Therefore, we recommend such models as the preferred model type for cheetahs.Results highlight the potential for mixture models in overcoming the challenges of capture probability heterogeneity and in particular their use with species where intrasexual behavioral differences exist.
- High value-added products from the orange juice industry waste. [Journal Article]
- WMWaste Manag 2018; 79:71-78
- An underutilized residue called Citrus Pulp of Floater (CPF), which causes problems during the industrial process of manufacturing of orange juice, was explored for the production of high value-added…
An underutilized residue called Citrus Pulp of Floater (CPF), which causes problems during the industrial process of manufacturing of orange juice, was explored for the production of high value-added products. Mixed, first (1G) and second generation (2G) ethanol, a clean and renewable biofuel, was obtained after an enzyme cocktail isolated from the Xanthomonas axonopodis pv. citri (Xac) was applied in hydrolysis of this biomass. Then, mono- and co-culture fermentations were performed using the yeast Saccharomyces cerevisiae and two Candida strains (Candida parapsilosis IFM 48375 and NRRL Y-12969), where the last two were isolated from the orange bagasse in natura. After the enzymatic hydrolysis step, sugars obtained were converted to ethanol achieving a yield of almost 100% after co-fermentation. Hesperidin, a flavonoid widely used for its antimicrobial and/or antioxidant activities, was also extracted from CPF by liquid-solid extraction and precipitation, with the yield of 1.2% and 92.6% pure. Finally, nanocellulose was produced through processes such as extraction, bleaching and nanonization with the yield of 1.4% and over 98% of purity. These products - ethanol, hesperidin and nanocellulose were obtained from three independent processes: (1) after an enzyme-based hydrolysis of CPF, liquid part was used for ethanol production, and solid was preserved; (2) hesperidin was isolated from a dry CPF, and solid residue was preserved; and (3) nanocellulose was obtained from the solid residues after processes cited in 1 and 2.
- [YAG laser vitreolysis for treatment of symptomatic vitreous opacities]. [Review]
- OOphthalmologe 2019; 116(1):73-84
- YAG laser vitreolysis is an innovative procedure to treat symptomatic vitreous floaters. The treatment decision is based on taking a comprehensive patient history, thorough clinical examination, real…
YAG laser vitreolysis is an innovative procedure to treat symptomatic vitreous floaters. The treatment decision is based on taking a comprehensive patient history, thorough clinical examination, realistic expectations, and detailed patient information including risk of the procedure. Manifestation of vitreous opacities and possible subjective impairments are considerably variable. Dynamic changes over time are possible. Therefore, not all vitreous opacities are suitable for laser treatment. A classification according to histoanatomical origin can aid the treatment decision. In comparison to other ophthalmic YAG laser applications, YAG laser vitreolysis differs predominantly by its specific technical equipment requirements. In addition, treatment is more complex and time consuming, requires appropriate experience and knowledge of the surgeon, and should be carried out with utmost care and accuracy.
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- Corneal epithelial toxicity with intravitreal methotrexate in a case of B-cell lymphoma with ocular involvement. [Case Reports]
- BCBMJ Case Rep 2018 Aug 27; 2018
- A 49-year-old woman, known case of diffuse large B-cell lymphoma, presented with complaints of floater in both eyes since 3 days. On examination, visual acuity was 0.18 logMAR in both eyes. Indirect …
A 49-year-old woman, known case of diffuse large B-cell lymphoma, presented with complaints of floater in both eyes since 3 days. On examination, visual acuity was 0.18 logMAR in both eyes. Indirect ophthalmoscopy revealed presence of vitreous clumps. Vitreous biopsy was done and the histopathological report suggested a diagnosis of ocular lymphoma. The patient was treated with weekly injections of intravitreal methotrexate in both eyes. The patient developed severe photophobia, watering, redness and diminution of vision in the both eyes 2 days following the fifth dose of intravitreal methotrexate. Severe limbitis with annular corneal epitheliopathy and corneal haze was noted on slit-lamp examination. The patient was started on topical lubricants, antibiotic, ciclosporin, loteprednol, folinic acid and oral folic acid. Complete resolution was noted at 2-week follow-up. The patient, however, refused further injections and was kept on close follow-up to look for recurrence of the disease.