- Ultrastructural characterization of cells in primary cultures from different adult tissues of Biomphalaria tenagophila TAIM, a strain that is absolutely resistant to Schistosoma mansoni infection. [Journal Article]
- MMicron 2014; 67:37-49
- The etiological agent of schistosomiasis in Brazil, Schistosoma mansoni, requires an obligatory passage through Biomphalaria snails to complete its life cycle. In these intermediate hosts, interactio…
The etiological agent of schistosomiasis in Brazil, Schistosoma mansoni, requires an obligatory passage through Biomphalaria snails to complete its life cycle. In these intermediate hosts, interaction with the parasite is mediated by humoral factors and hemocytes by mechanisms that are not yet fully understood. Extant studies exploring these processes are usually conducted through experimental infection of Biomphalaria with S. mansoni miracidia. Thus, tissue-derived cultures of Biomphalaria may be useful in increasing the understanding of that interaction at cellular level. However, in the absence of morphological characterization of those cells in culture, the application of such models is delayed. In the present work, we cultured different tissues of B. tenagophila, the second most important host of S. mansoni in Brazil, using a strain that is naturally and absolutely resistant to S. mansoni infection. This decision was driven by the view that this strain might be provided with the most effective response against parasite infection. Primary cultures were successfully established from nine Biomphalaria tissues and the respective cells in culture were ultra structurally described. Attention was particularly devoted to cells derived from mantle cavity and kidney tissues. Although they have been considered important centers for hemocyte production in Biomphalaria, no detailed cell characterization is available in the pertinent literature. Herein, kidney-derived cells partially shared hematoblast characteristics. Moreover, under optical microscopy, kidney cells in culture were very similar to those derived from amebocyte-producing organ (APO) cultures, which have been recently shown to be capable of eliminating S. mansoni sporocysts in vitro. Based on the close resemblance of those cultures and their anatomical proximity inside the mantle cavity, we suggest the effective participation of Biomphalaria kidney cells in hematopoiesis and in host response to S. mansoni infection.
- [A clinical study on leucogen tablets therapy efficacy during PEG-alpha interferon and alpha-interferon in chronic hepatitis B]. [Journal Article]
- ZSZhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2012; 26(2):111-3
- CONCLUSIONS: Leucogen tablets treatment and prevention interferon-alpha-related neutrophil counts hematological reaction in CHB treated with alpha-interferon, and had the trend of reducing interferon-alpha-related hematoblast decrease, farther improved the efficacy of alpha-interferon treatment CHB.
- [Meta analysis of compound matrine injection combined with cisplatin chemotherapy for advanced gastric cancer]. [Meta-Analysis]
- ZZZhongguo Zhong Yao Za Zhi 2011; 36(22):3198-202
- CONCLUSIONS: Compared with cisplatin chemotherapy, compound matrine injection combined with cisplatin chemotherapy can significantly improve the efficiency, QOL and myelosuppression, and reduce adverse events.
- [A meta analysis of aidi injection plus taxotere and cisplatin in the treatment of non-small cell lung cancer]. [Meta-Analysis]
- ZFZhongguo Fei Ai Za Zhi 2010; 13(11):1027-34
- CONCLUSIONS: Compared with TP chemotherapy alone, the combination can significantly improve the efficiency, QOL and myelosuppression, and reduce adverse events.
- The discovery of the hematoblast by Carl Heitzmann (1836-1896) in 1872. [Historical Article]
- IJInt J Dermatol 2000; 39(8):632-5
- The description of the hematoblast by the dermatopathologist Carl Heitzmann in 1872. [Historical Article]
- JIJ Invest Dermatol 1999; 113(5):861-2
- THE RELATION OF CHROMATIN TO HEMOGLOBIN AND BILIRUBIN. [Journal Article]
- JEJ Exp Med 1929 May 31; 49(6):937-43
- 1. Attention is directed to the diversity of opinion among investigators regarding the site and manner of hemoglobin formation in the body and its relation to bile pigment metabolism. 2. It is probab…
1. Attention is directed to the diversity of opinion among investigators regarding the site and manner of hemoglobin formation in the body and its relation to bile pigment metabolism. 2. It is probable that in forming new hypotheses on this subject the earlier work of A. B. Macallum on the relation of chromatin to hemoglobin formation has not received sufficient consideration. 3. It has been shown by means of microchemical iron stains of the bone marrow cells, that the iron content of the hematoblast is increased during rapid hemoglobin production in simple anemia. 4. This fact is compatible with the work of Macallum who believed that hemoglobin is derived from the chromatin of the hematoblast. It does not support a theory that hemoglobin is formed as a part of a circulating pigment. 5. It is suggested that bilirubin is derived from the chromatin of body cells through the intermediary stages of the respiratory pigments, hemoglobin and cytochrome, from erythrocytes and other cells, respectively.