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16,526 results
  • Mass Spectrometry-compatible Subcellular Fractionation for Proteomics. [Journal Article]
    J Proteome Res 2019Masuda T, Sugiyama N, … Ishihama Y
  • We found that nuclear envelopes stabilize against surfactants in the presence of ethylene glycol (EG). We, therefore, developed a novel subcellular fractionation approach for proteomics using RIPA buffer containing EG and phase transfer surfactants. This method involves separating the cells into the cytoplasm, organelles, and nucleus, including intermediate filaments without ultracentrifugation. …
  • Types of surgery post-neoadjuvant chemotherapy for pleural mesothelioma. [Journal Article]
    Expert Rev Respir Med 2019Kuribayashi K, Doi H, Kijima T
  • Introduction: The surgical arm of the Mesothelioma and Radical Surgery (MARS) trial involved a multimodal approach, with combined therapy consisting of chemotherapy, complete gross resection, and radiation therapy. However, the MARS trial did not compare surgery with chemotherapy, and the survival and quality of life outcomes of this trial's surgical arm were inferior to those of the non-surgical…
  • In vitro reconstitution of non-phosphorylative Entner-Doudoroff pathway for lactate production. [Journal Article]
    J Biosci Bioeng 2019Okano K, Zhu Q, Honda K
  • In vitro metabolic engineering is an emerging framework for bioproduction systems, in which synthetic metabolic pathways are constructed using a limited number of enzymes. Employment of thermophilic enzymes as catalytic elements in pathways enables the use of simple heat purification of recombinantly expressed enzymes. However, thermophilic enzymes are generally incompatible with thermo-labile su…
  • Targeted cell ablation-based insights into wound healing and restorative patterning. [Review]
    Curr Opin Plant Biol 2019; 52:124-130Hoermayer L, Friml J
  • Plants as sessile organisms are constantly under attack by herbivores, rough environmental situations, or mechanical pressure. These challenges often lead to the induction of wounds or destruction of already specified and developed tissues. Additionally, wounding makes plants vulnerable to invasion by pathogens, which is why wound signalling often triggers specific defence responses. To stay comp…
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