- Reactivity, Intensity, Polarity and Stability questionnaire (RIPoSt-40) assessing emotional dysregulation: Development, reliability and validity. [Journal Article]
- JAJ Affect Disord 2019 Jul 05; 257:187-194
- CONCLUSIONS: The non-clinical sample was recruited through a web-survey and mainly included young and highly educated subjects. Mood and anxiety comorbidity of the clinical samples were not taken into consideration.RIPoSt-40 questionnaire has proved to be a valid, reliable and useful tool to assess ED both in clinical and non-clinical contexts.
- Assessment and validation of groundwater vulnerability to nitrate in porous aquifers based on a DRASTIC method modified by projection pursuit dynamic clustering model. [Journal Article]
- JCJ Contam Hydrol 2019 Jul 04; 226:103522
- The groundwater vulnerability assessment has been identified as an important tool of groundwater pollution prevention. In this study, the DRASTIC method was modified to estimate the groundwater vulne…
The groundwater vulnerability assessment has been identified as an important tool of groundwater pollution prevention. In this study, the DRASTIC method was modified to estimate the groundwater vulnerability of porous aquifers to nitrate. The parameter system was optimized by replacing the original parameters of aquifer media, soil media, impact of the vadose zone and hydraulic conductivity with quantitative parameters of aquifer thickness, nitrate attenuation intensity, hydraulic resistance and groundwater velocity and adding pollutant input intensity to develop the DRANTHVP method. In addition, the rating scale of the DRASTIC method was revised, and the weight and influence degree of each parameter were determined using the projection pursuit dynamic clustering (PPDC) model. The DRANTHVP method was applied to the plain area of western Jilin Province, and the vulnerability index map of groundwater to nitrate pollution was established. The DRANTHVP method improved the correlation coefficient between vulnerability index and nitrate concentration to 0.720, which was more than three times that of the original DRASTIC method. And the high variance of the (ANOVA) F statistic indicated that there was less overlap between the nitrate values in different vulnerability classes. Furthermore, the DRANTHVP method produced a more uniform distribution of vulnerability classes. In short, the DRANTHVP method using the PPDC model was suitable to assess the groundwater vulnerability of porous aquifers to nitrate in the study area. Hence, the mapping of groundwater vulnerability to nitrate can provide a scientific and reasonable basis for the formulation of groundwater resource protection plan.
- Detecting acetylated aminoacids in blood serum using hyperpolarized 13C-1Η-2D-NMR. [Journal Article]
- JMJ Magn Reson 2019 Jul 05; 305:175-179
- Dynamic Nuclear Polarization (DNP) can substantially enhance the sensitivity of NMR experiments. Among the implementations of DNP, ex-situ dissolution DNP (dDNP) achieves high signal enhancement leve…
Dynamic Nuclear Polarization (DNP) can substantially enhance the sensitivity of NMR experiments. Among the implementations of DNP, ex-situ dissolution DNP (dDNP) achieves high signal enhancement levels owing to a combination of a large temperature factor between 1.4 and 300 K with the actual DNP effect in the solid state at 1.4 K. For sufficiently long T1 relaxation times much of the polarization can be preserved during dissolution with hot solvent, thus enabling fast experiments during the life time of the polarization. Unfortunately, for many metabolites found in biological samples such as blood, relaxation times are too short to achieve a significant enhancement. We have therefore introduced 13C-carbonyl labeled acetyl groups as probes into amino acid metabolites using a simple reaction protocol. The advantage of such tags is a sufficiently long T1 relaxation time, the possibility to enhance signal intensity by introducing 13C, and the possibility to identify tagged metabolites in NMR spectra. We demonstrate feasibility for mixtures of amino acids and for blood serum. In two-dimensional dDNP-enhanced HMQC experiments of these samples acquired in 8 s we can identify acetylated amino acids and other metabolites based on small differences in chemical shifts.
- Long-term exposure to increasing temperatures on scleractinian coral fragments reveals oxidative stress. [Journal Article]
- MEMar Environ Res 2019 Jul 08; 150:104758
- Global warming is leading to increases in tropical storms' frequency and intensity, allowing fragmentation of reef-forming coral species, but also to coral bleaching and mortality. The first level of…
Global warming is leading to increases in tropical storms' frequency and intensity, allowing fragmentation of reef-forming coral species, but also to coral bleaching and mortality. The first level of organism's response to an environmental perturbation occurs at the cellular level. This study investigated the long-term oxidative stress on fragments of nine Indo-Pacific reef-forming coral species exposed for 60 days to increasing temperatures (30 °C and 32 °C) and compared results with control temperature (26 °C). Coral overall condition (appearance), lipid peroxidation (LPO), catalase activity (CAT), and glutathione S-transferase (GST) were assessed. The species Turbinaria reniformis, Galaxea fascicularis, and Psammocora contigua were the most resistant to heat stress, presenting no oxidative damage at 30 °C. Unlike G. fasciularis, both T. reniformis and P. contigua showed no evidence of oxidative damage at 32 °C. All remaining species' fragments died at 32 °C. Stylophora pistillata and Pocillopora damicornis were the most susceptible species to heat stress, not resisting at 30 °C.
- Interleukin 6 Dependent Synaptic Plasticity in a Social Defeat-Susceptible Prefrontal Cortex Circuit. [Journal Article]
- NNeuroscience 2019 Jul 10
- The role of the pro-inflammatory cytokine interleukin-6 (IL-6) in the etiology of stress-induced synaptic plasticity is yet unknown. We took advantage of a genetically modified mouse (TG) in which IL…
The role of the pro-inflammatory cytokine interleukin-6 (IL-6) in the etiology of stress-induced synaptic plasticity is yet unknown. We took advantage of a genetically modified mouse (TG) in which IL-6 trans-signaling via the soluble IL-6 receptor was blocked, to determine the role of IL-6 trans-signaling in the effects of a Social Defeat protocol (SD) on synaptic function of the medial prefrontal cortex (mPFC). Synaptic function in stress-sensitive (S) and stress-resilient (R) animals was studied in a mPFC slice preparation with whole-cell patch-clamp recording. SD altered numerous synaptic properties of the mPFC: R WT (but not TG) displayed a decreased ratio between N methyl-D-aspartate receptor (NMDAR-) dependent and amino propionic acid receptor (AMPAR-) dependent-current (INMDA/IAMPA), while S WT animals (but not TG) showed a reduced ratio between AMPA and γ-amino-butyric acid receptor type A (GABAAR)-dependent currents (IAMPA/IGABA). Also, SD induced an increase in the frequency but a decrease in the amplitude of excitatory action-potential dependent PSCs (sEPSCs), both in an IL-6 dependent manner, as well as a generalized (S/R-independent) decrease in the frequency of action potential independent (miniature) excitatory (IL-6 dependent) as well as inhibitory (IL-6 independent) postsynaptic current frequency. Interestingly, corner preference (measuring the intensity of social defeat) correlated positively with INMDA/IAMPA and eEPSC frequency and negatively with IAMPA/IGABA. Our results suggest that SD induces behaviorally-relevant synaptic rearrangement in mPFC circuits, part of which is IL-6 dependent. In particular, IL-6 is necessary to produce synaptic plasticity leading to stress resilience in some individuals, but to stress sensitivity in others.
- Cholesterol lowering and stroke: no longer room for pleiotropic effects of statins - confirmation from PCSK9 inhibitor studies. [Journal Article]
- AJAm J Med 2019 Jul 10
- CONCLUSIONS: These findings offer a definitive proof that the pure total (and low-density lipoprotein) cholesterol lowering, with any available lipid-lowering intervention, reduce stroke risk proportional to the extent of cholesterol reduction, without the need of invoking "pleiotropic" effects of any such treatment.
- Non-invasive Staging of Pressure Ulcers Using Photoacoustic Imaging. [Journal Article]
- WRWound Repair Regen 2019 Jul 13
- Ulcers including pressure ulcers and diabetic foot ulcers damage the skin and underlying tissue in people with compromised blood circulation. They are classified into four stages of severity and span…
Ulcers including pressure ulcers and diabetic foot ulcers damage the skin and underlying tissue in people with compromised blood circulation. They are classified into four stages of severity and span from mild reddening of the skin to tissue damage and muscle/bone infections. Here, we used photoacoustic imaging as a non-invasive method for detecting early tissue damage that cannot be visually observed while also staging the disease using quantitative image analysis. We used a mouse model of pressure ulcers by implanting sub-dermal magnets in the dorsal flank and periodically applying an external magnet to the healed implant site. The magnet-induced pressure was applied in cycles, and the extent of ulceration was dictated by the number of cycles. We used both laser- and LED-based photoacoustic imaging tools with 690 nm excitation to evaluate the change in photoacoustic signal and depth of injury. Using laser-based photoacoustic imaging system, we found a 4.4-fold increase in the photoacoustic intensity in stage I versus baseline (no pressure). We also evaluated the depth of injury using photoacoustics. We measured a photoacoustic ulcer depth of 0.38 ± 0.09 mm, 0.74 ± 0.11 mm, 1.63 ± 0.4 mm, and 2.7 ± 0.31 mm (n=4) for stages I, II, III, and IV, respectively. The photoacoustic depth differences between each stage were significant (p < 0.05). We also used an LED-based photoacoustic imaging system to detect early stage (stage I) pressure ulcers and observed a 2.5-fold increase in photoacoustic signal. Importantly, we confirmed the capacity of this technique to detect dysregulated skin even before stage I ulcers have erupted. We also observed significant changes in photoacoustic intensity during healing suggesting that this approach can monitor therapy. These findings were confirmed with histology. These results suggest that this photoacoustic-based approach might have clinical value for monitoring skin diseases including pressure ulcers. This article is protected by copyright. All rights reserved.
- The generation of a comprehensive spectral library for the analysis of the guinea pig proteome by SWATH-MS. [Journal Article]
- PProteomics 2019 Jul 13; :e1900156
- Advances in liquid chromatography-mass spectrometry have facilitated the incorporation of proteomic studies to many biology experimental workflows. Data-Independent Acquisition platforms, such as SWA…
Advances in liquid chromatography-mass spectrometry have facilitated the incorporation of proteomic studies to many biology experimental workflows. Data-Independent Acquisition platforms, such as SWATH, offer several advantages for label-free quantitative assessment of complex proteomes over Data-Dependent Acquisition (DDA) approaches. However, SWATH data interpretation requires spectral libraries as a detailed reference resource. The guinea pig (Cavia porcellus) is an excellent experimental model for translation to many aspects of human physiology and disease yet there is limited experimental information regarding its proteome. To overcome this knowledge gap, we generated a comprehensive spectral library of the guinea pig proteome. Homogenates and tryptic digests were prepared from 16 tissues and subjected to >200 DDA runs. Analysis of >250,000 peptide-spectrum matches resulted in a library of 73594 peptides from 7666 proteins. Library validation was provided by (i) analysing externally-derived SWATH files (https://doi.org/10.1016/j.jprot.2018.03.023) and comparing peptide intensity quantifications; (ii) merging of externally-derived data to the base library. This furnishes the research community with a comprehensive proteomic resource that will facilitate future molecular-phenotypic studies using (re-engaging) the guinea pig as an experimental model of relevance to human biology. The spectral library and raw data are freely accessible in the MassIVE repository (MSV000083199). This article is protected by copyright. All rights reserved.
- End-of-Life Decision Making and Treatment for Patients with Professional Guardians. [Journal Article]
- JAJ Am Geriatr Soc 2019 Jul 13
- CONCLUSIONS: Rates of high-intensity treatment and hospice care were similar to older adults overall. Because high-intensity treatment was less likely when the guardian had information about a patient's preferences, future work should focus on advance care planning for individuals without an appropriate surrogate.
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- Effects of anodal transcranial direct current stimulation on motor evoked potentials variability in humans. [Journal Article]
- PRPhysiol Rep 2019; 7(13):e14087
- Motor evoked potentials (MEPs) obtained from transcranial magnetic stimulation (TMS) allow corticospinal excitability (CSE) to be measured in the human primary motor cortex (M1). CSE responses to tra…
Motor evoked potentials (MEPs) obtained from transcranial magnetic stimulation (TMS) allow corticospinal excitability (CSE) to be measured in the human primary motor cortex (M1). CSE responses to transcranial direct current stimulation (tDCS) protocols are highly variable. Here, we tested the reproducibility and reliability of individual MEPs following a common anodal tDCS protocol. In this study, 32 healthy subjects received anodal tDCS stimulation over the left M1 for three durations (tDCS-T5, tDCS-T10, and tDCS-T20 min) on separate days in a crossover-randomized order. After the resting motor threshold (RMT) was determined for the contralateral first dorsal interosseous muscle, 15 single pulses 4-8 sec apart at an intensity of 120% RMT were delivered to the left M1 to determine the baseline MEP amplitude at T0 , T5 , T10 , T20 , T30 , T40 , T50 , and T60 min after stimulation for each durations. During TMS delivery, 3D images of the participant's cortex and hot spot were visualized for obtaining MEPs from same position. Our findings revealed that there was a significant MEPs improvement at T0 (P = 0.01) after 10 min of anodal stimulation. After the 20-min stimulation duration, MEPs differed specifically at T0, T5, T30 min (P < 0.05). This indicates that tDCS is a promising tool to improve MEPs. Our observed variability in response to the tDCS protocol is consistent with other noninvasive brain stimulation studies.