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50 results
  • Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image Cytometry. [Journal Article]
    Methods Mol Biol 2017; 1601:27-41Chan LL, McCulley KJ, Kessel SL
  • The ability to accurately measure cell viability is important for any cell-based assay. Traditionally, viability measurements have been performed using the trypan blue exclusion method on a hemacytometer, which allows researchers to visually distinguish viable from nonviable cells. While the trypan blue method can work for cell lines or primary cells that have been rigorously purified, in more co…
  • The dendritic cell receptor Clec9A binds damaged cells via exposed actin filaments. [Journal Article]
    Immunity 2012; 36(4):646-57Zhang JG, Czabotar PE, … Lahoud MH
  • The immune system must distinguish viable cells from cells damaged by physical and infective processes. The damaged cell-recognition molecule Clec9A is expressed on the surface of the mouse and human dendritic cell subsets specialized for the uptake and processing of material from dead cells. Clec9A recognizes a conserved component within nucleated and nonnucleated cells, exposed when cell membra…
  • Comparative gene evolution in haemosporidian (apicomplexa) parasites of birds and mammals. [Journal Article]
    Mol Biol Evol 2010; 27(3):537-42Outlaw DC, Ricklefs RE
  • Haemosporidian parasites of birds and mammals reproduce asexually inside nucleated and nonnucleated host erythrocytes, respectively. Because of these different parasite environments and because bird parasites are paraphyletic, we evaluated whether patterns of parasite molecular evolution differ between host groups. We compared two mitochondrial (mt) genes and one apicoplast gene across mammal Pla…
  • [Platelets "Toll-like receptor" engagement stimulates the release of immunomodulating molecules]. [Review]
    Transfus Clin Biol 2008; 15(4):139-47Cognasse F, Hamzeh-Cognasse H, Garraud O
  • Platelets are nonnucleated cellular elements that play a role in the process of haemostasis, and also in various ways in innate immunity and in inflammation. Platelets also contain numerous secretory products and can exert critical roles in several aspects of haemostasis. In addition, they house and secrete a variety of cytokines, chemokines and associated molecules which behave as ligands for re…
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