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22,693 results
  • Synthesis and polymerase recognition of a pyrrolocytidine TNA triphosphate. [Journal Article]
    Biopolymers. 2020 Jul 02 [Online ahead of print]Mei H, Wang Y, … Chaput JC
  • Synthetic genetics is an area of synthetic biology that aims to extend the properties of heredity and evolution to artificial genetic polymers, commonly known as xeno-nucleic acids or XNAs. In addition to establishing polymerases that are able to convert genetic information back and forth between DNA and XNA, efforts are underway to construct XNAs with expanded chemical functionality. α-L-Threose…
  • Improved Method for Transformation of Vibrio vulnificus by Electroporation. [Journal Article]
    Curr Protoc Microbiol. 2020 Sep; 58(1):e106.Jayakumar JM, Shapiro OH, Almagro-Moreno S
  • Vibrio vulnificus, an emergent human pathogen, causes fulminant septicemia with a mortality rate of over 50%. Unlike for other pathogenic Vibrio species, the factors to conclusively indicate the virulence potential of V. vulnificus strains remain largely unknown. Understanding the pathogenesis of this bacterium at a molecular level is severely hindered by inefficiencies in transformation, for ins…
  • Highly efficient generation of sheep with a defined FecBB mutation via adenine base editing. [Journal Article]
    Genet Sel Evol. 2020 Jul 01; 52(1):35.Zhou S, Ding Y, … Chen Y
  • Base editing has the potential to improve important economic traits in agriculture and can precisely convert single nucleotides in DNA or RNA sequences into minimal double-strand DNA breaks (DSB). Adenine base editors (ABE) have recently emerged as a base editing tool for the conversion of targeted A:T to G:C, but have not yet been used in sheep. ABEmax is one of the latest versions of ABE, which…
  • Reprogramming acetogenic bacteria with CRISPR-targeted base editing via deamination. [Journal Article]
    ACS Synth Biol. 2020 Jul 01 [Online ahead of print]Xia PF, Casini I, … Molitor B
  • Acetogenic bacteria are rising in popularity as chassis microbes in biotechnology due to their capability of converting inorganic one-carbon (C1) gases to organic chemicals. To fully uncover the potential of acetogenic bacteria, synthetic-biology tools are imperative to either engineer designed functions or to interrogate the physiology. Here, we report a genome-editing tool at a one-nucleotide r…
  • Processing by RNase 1 forms tRNA halves and distinct Y RNA fragments in the extracellular environment. [Journal Article]
    Nucleic Acids Res. 2020 Jul 01 [Online ahead of print]Nechooshtan G, Yunusov D, … Gingeras TR
  • Extracellular RNAs participate in intercellular communication, and are being studied as promising minimally invasive diagnostic markers. Several studies in recent years showed that tRNA halves and distinct Y RNA fragments are abundant in the extracellular space, including in biofluids. While their regulatory and diagnostic potential has gained a substantial amount of attention, the biogenesis of …
  • The delivery challenge: fulfilling the promise of therapeutic genome editing. [Review]
    Nat Biotechnol. 2020 Jun 29 [Online ahead of print]van Haasteren J, Li J, … Schaffer DV
  • Genome editing has the potential to treat an extensive range of incurable monogenic and complex diseases. In particular, advances in sequence-specific nuclease technologies have dramatically accelerated the development of therapeutic genome editing strategies that are based on either the knockout of disease-causing genes or the repair of endogenous mutated genes. These technologies are progressin…
  • Small-molecule sensitization of RecBCD helicase-nuclease to a Chi hotspot-activated state. [Journal Article]
    Nucleic Acids Res. 2020 Jun 29 [Online ahead of print]Karabulut AC, Cirz RT, … Smith GR
  • Coordinating multiple activities of complex enzymes is critical for life, including transcribing, replicating and repairing DNA. Bacterial RecBCD helicase-nuclease must coordinate DNA unwinding and cutting to repair broken DNA. Starting at a DNA end, RecBCD unwinds DNA with its fast RecD helicase on the 5'-ended strand and its slower RecB helicase on the 3'-ended strand. At Chi hotspots (5' GCTGG…
  • Tetramerisation of the CRISPR ring nuclease Crn3/Csx3 facilitates cyclic oligoadenylate cleavage. [Journal Article]
    Elife. 2020 Jun 29; 9Athukoralage JS, McQuarrie S, … White MF
  • Type III CRISPR systems detect foreign RNA and activate the cyclase domain of the Cas10 subunit, generating cyclic oligoadenylate (cOA) molecules that act as a second messenger to signal infection, activating nucleases that degrade the nucleic acid of both invader and host. This can lead to dormancy or cell death; to avoid this, cells need a way to remove cOA from the cell once a viral infection …
  • Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA. [Journal Article]
    Sci Adv. 2020 Jun; 6(25):eaaz4849.Saha C, Mohanraju P, … Louwen R
  • CRISPR-Cas9 systems are enriched in human pathogenic bacteria and have been linked to cytotoxicity by an unknown mechanism. Here, we show that upon infection of human cells, Campylobacter jejuni secretes its Cas9 (CjeCas9) nuclease into their cytoplasm. Next, a native nuclear localization signal enables CjeCas9 nuclear entry, where it catalyzes metal-dependent nonspecific DNA cleavage leading to …
  • Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells. [Journal Article]
    Front Plant Sci. 2020; 11:816.Bennett M, Deikman J, … Iandolino A
  • Foliar application of dsRNA to elicit an RNA interference (RNAi) response is currently under consideration as a crop protection strategy. To access the RNAi machinery of a plant, foliarly applied dsRNAs must traverse the plant cuticle, avoid nuclease degradation, and penetrate the cell wall and plasma membrane. Application methods and co-formulants have been identified by Bayer Crop Science resea…
  • Therapeutic Manipulation of mtDNA Heteroplasmy: A Shifting Perspective. [Review]
    Trends Mol Med. 2020 Jul; 26(7):698-709.Jackson CB, Turnbull DM, … Gammage PA
  • Mutations of mitochondrial DNA (mtDNA) often underlie mitochondrial disease, one of the most common inherited metabolic disorders. Since the sequencing of the human mitochondrial genome and the discovery of pathogenic mutations in mtDNA more than 30 years ago, a movement towards generating methods for robust manipulation of mtDNA has ensued, although with relatively few advances and some controve…
  • Detection and Modulation of DNA Translocations During Multi-Gene Genome Editing in T Cells. [Journal Article]
    CRISPR J. 2020 Jun; 3(3):177-187.Bothmer A, Gareau KW, … Cotta-Ramusino C
  • Multiplexed genome editing with DNA endonucleases has broad application, including for cellular therapies, but chromosomal translocations, natural byproducts of inducing simultaneous genomic breaks, have not been explored in detail. Here we apply various CRISPR-Cas nucleases to edit the T cell receptor alpha and beta 2 microglobulin genes in human primary T cells and comprehensively evaluate the …
  • Characterization of a Type II-A CRISPR-Cas System in Streptococcus mutans. [Journal Article]
    mSphere. 2020 Jun 24; 5(3)Mosterd C, Moineau S
  • Streptococcus mutans and its virulent phages are important members of the human oral microbiota. S. mutans is also the primary causal agent of dental caries. To survive in this ecological niche, S. mutans must encode phage defense mechanisms, which include CRISPR-Cas systems. Here, we describe the CRISPR-Cas type II-A system of S. mutans strain P42S, which was found to display natural adaptation …
  • The iron-sulphur cluster in human DNA2 is required for all biochemical activities of DNA2. [Journal Article]
    Commun Biol. 2020 Jun 23; 3(1):322.Mariotti L, Wild S, … Gari K
  • The nuclease/helicase DNA2 plays important roles in DNA replication, repair and processing of stalled replication forks. DNA2 contains an iron-sulphur (FeS) cluster, conserved in eukaryotes and in a related bacterial nuclease. FeS clusters in DNA maintenance proteins are required for structural integrity and/or act as redox-sensors. Here, we demonstrate that loss of the FeS cluster affects bindin…
  • Efficient self-assembly of heterometallic triangular necklace with strong antibacterial activity. [Journal Article]
    Nat Commun. 2020 Jun 23; 11(1):3178.Wu GY, Shi X, … Yang HB
  • Sophisticated mechanically interlocked molecules (MIMs) with interesting structures, properties and applications have attracted great interest in the field of supramolecular chemistry. We herein report a highly efficient self-assembly of heterometallic triangular necklace 1 containing Cu and Pt metals with strong antibacterial activity. Single-crystal X-ray analysis shows that the finely arranged…
  • [Advances in gene editing of Corynebacterium glutamate]. [Review]
    Sheng Wu Gong Cheng Xue Bao. 2020 May 25; 36(5):820-828.Yang J, Ma X, … Lu F
  • Corynebacterium glutamicum, an important microorganism to produce amino acids and organic acids, has been widely applied in food and medicine fields. Therefore, using editing tools to study the function of unknown genes in C. glutamicum has great significance for systematic development of industrial strain with efficient and novel production capability. Recently, gene editing has been greatly dev…
  • CRISPR Genome Editing Technology and its Application in Genetic Diseases: A Review. [Journal Article]
    Curr Pharm Biotechnol. 2020 Jun 21 [Online ahead of print]Khatibi S, Sahebkar A, Bakhtiari SHA
  • Gene therapy has been a long-lasting goal of scientists and there are many ideal methods and tools that have been developed to correct disease-causing mutations in human. Recently, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology has been progressively used for the assessment or treatment of human diseases, including Thalassemia, Parkinson disease, Cystic Fibrosis, Gl…
  • Position of Deltaproteobacteria Cas12e nuclease cleavage sites depends on spacer length of guide RNA. [Journal Article]
    RNA Biol. 2020 Jun 21 [Online ahead of print]Selkova P, Vasileva A, … Fedorova I
  • Cas12e proteins (formerly CasX) form a distinct subtype of Class II type V CRISPR-Cas effectors. Recently, it was shown that DpbCas12e from Deltaproteobacteria and PlmCas12e from Planctomycetes can introduce programmable double-stranded breaks in mammalian genomes. Thus, along with Cas9 and Cas12a Class II effectors, Cas12e could be harnessed for genome editing and engineering. The location of cl…
  • Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits. [Journal Article]
    Nat Commun. 2020 Jun 19; 11(1):3138.Contreras-Llano LE, Meyer C, … Tan C
  • Synthetic biology has focused on engineering genetic modules that operate orthogonally from the host cells. A synthetic biological module, however, can be designed to reprogram the host proteome, which in turn enhances the function of the synthetic module. Here, we apply this holistic synthetic biology concept to the engineering of cell-free systems by exploiting the crosstalk between metabolic n…
  • CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis. [Journal Article]
    ACS Synth Biol. 2020 Jul 01 [Online ahead of print]Yu S, Price MA, … Wang M
  • Base editing technology based on clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) is a recent addition to the family of CRISPR technologies. Compared with the traditional CRISPR/Cas9 technology, it does not rely on DNA double strand break and homologous recombination, and can realize gene inactivation and point mutation more quickly and simply. Herein, …
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