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244 results
  • Protamine withdrawal from human sperm nuclei following heterologous ICSI into hamster oocytes. [Journal Article]
    Protein Pept Lett. 2011 Aug; 18(8):811-6.Jones EL, Zalensky AO, Zalenskaya IA
  • During late stages of spermatogenesis in mammals, most histones bound to DNA are replaced by protamines (PRM), which results in formation of supercondensed and genetically inert sperm chromatin. At fertilization, mature spermatozoon penetrates oocyte and chromatin is remodeled "back" from nucleoprotamine to nucleohistone state. While being crucial for activation of male genome and ultimately for …
  • Spermatozoal RNAs: what about their functions? [Review]
    Microsc Res Tech. 2009 Aug; 72(8):536-51.Dadoune JP
  • The profound architectural changes that transform spermatids into spermatozoa result in a high degree of DNA packaging within the sperm head. However, the mature sperm chromatin that harbors imprinted genes exhibits a dual nucleoprotamine/nucleohistone structure with DNase-sensitive regions, which could be implicated in the establishment of efficient epigenetic information in the developing embry…
  • Sperm chromatin released by nucleases. [Journal Article]
    Syst Biol Reprod Med. 2008 Jan-Feb; 54(1):37-46.Nazarov IB, Shlyakhtenko LS, … Zalensky AO
  • In human spermatozoa, 15-20% of histones are retained in the nucleus to coexist with protamines. Hypothetically, nucleohistone regions of sperm chromatin mark DNA sequences for distinctive processing during fertilization and early embryogenesis. The structural organization and molecular composition of nucleohistones in human spermatozoa is poorly studied. Here, we isolate and characterize fractio…
  • Relationship between chromatin organization, mRNAs profile and human male gamete quality. [Review]
    Asian J Androl. 2007 Sep; 9(5):587-92.Galeraud-Denis I, Lambard S, Carreau S
  • Spermiogenesis is a complex process leading to the formation of motile spermatozoa characterized by a highly stable chromatin compaction that transfers the paternal genome into the oocyte. It is commonly held that these haploid cells are devoid of transcriptional and translational activities and that the transcripts represent remnants of stored mRNAs. Recently, the chromatin organization of matur…
  • Protamines and male infertility. [Review]
    Hum Reprod Update. 2006 Jul-Aug; 12(4):417-35.Oliva R
  • Protamines are the major nuclear sperm proteins. The human sperm nucleus contains two types of protamine: protamine 1 (P1) encoded by a single-copy gene and the family of protamine 2 (P2) proteins (P2, P3 and P4), all also encoded by a single gene that is transcribed and translated into a precursor protein. The protamines were discovered more than a century ago, but their function is not yet full…
  • DNA aggregating protein from carp (Cyprinus capio) eggs. [Journal Article]
    Biol Pharm Bull. 2005 Jan; 28(1):13-8.Hosono M, Sugawara S, … Nitta K
  • Hemagglutinating activity for glutaraldehyde-fixed, trypsinized human and rabbit erythrocytes was obtained from carp (Ciprinus capio) egg extract. This activity was inhibited by sulfated glycans such as heparin and fucoidan, and salmon testis DNA (stDNA), but not by mono- and oligosaccharides. The active fraction from hydroxyapatite column, HA400, caused aggregation of stDNA at a concentration of…
  • Theoretical studies of the cross-linking mechanisms between cytosine and tyrosine. [Journal Article]
    J Am Chem Soc. 2002 Mar 20; 124(11):2753-61.Ban F, Lundqvist MJ, … Eriksson LA
  • DNA-protein cross-linking is one of the many DNA lesions mediated by hydroxyl radicals, the most damaging among the reactive oxygen species in biological systems. Density functional theory methods are employed to investigate the complex reaction mechanisms of the formation of cytosine-tyrosine cross-links as observed in gamma-irradiated aqueous solutions of cytosine and tyrosine, as well as in ga…
  • Counter-proliferative effects of nucleosomal antigens in cultures from lupus patients. [Journal Article]
    Lupus. 2001; 10(5):332-9.Salaman MR, Mawer DP, … Isenberg DA
  • Blood mononuclear cells from 20 lupus patients were cultured in the presence of nucleosomal antigens to determine whether they induce lymphocyte proliferation. The predominant effect seen, however, was one of inhibition of the background proliferation. Such inhibition was rare with cells from female or male controls. Nucleohistone (NH), crude histone and enriched preparations of histones H2A/H4, …
  • Chromatin structure of telomere domain in human sperm. [Journal Article]
    Biochem Biophys Res Commun. 2000 Dec 09; 279(1):213-8.Zalenskaya IA, Bradbury EM, Zalensky AO
  • Telomeres in human sperm nucleus are clustered at the nuclear periphery. Chromosomes in the sperm are highly condensed with protamines, however, a small portion of DNA remains associated with histones; the role of the nucleohistone is unknown. To examine structure of the telomeric chromatin, the sperm nuclei were treated with micrococcal nuclease. Chromatin released by the digestion was free from…
  • Influence of nucleosome structure on the three-dimensional folding of idealized minichromosomes. [Journal Article]
    Structure. 1999 Aug 15; 7(8):1009-22.Martino JA, Katritch V, Olson WK
  • CONCLUSIONS: The resulting models with 4-24 bound nucleosomes illustrate how a minichromosome can adopt the low-writhe folding motifs deduced from biochemical studies, and account for published images of the 30 nm chromatin fiber and the simian virus 40 (SV40) nucleohistone core. The marked sensitivity of global folding to the degree of protein-DNA interactions and the assumed nucleosomal shape suggest potential mechanisms for chromosome rearrangements upon histone modification.
  • Mechanism of transcription through the nucleosome by eukaryotic RNA polymerase. [Journal Article]
    Science. 1997 Dec 12; 278(5345):1960-3.Studitsky VM, Kassavetis GA, … Felsenfeld G
  • Nucleosomes, the nucleohistone subunits of chromatin, are present on transcribed eukaryotic genes but do not prevent transcription. It is shown here that the large yeast RNA polymerase III transcribes through a single nucleosome. This takes place through a direct internal nucleosome transfer in which histones never leave the DNA template. During this process, the polymerase pauses with a pronounc…
  • Generation of hydroxyl radicals by nucleohistone-bound metal-adriamycin complexes. [Journal Article]
    Free Radic Res. 1996 Sep; 25(3):207-20.Chakrabarti S, Mahmood A, … Makrigiorgos GM
  • A recently developed method has been utilized to demonstrate the generation of hydroxyl radicals (HO.) in the immediate proximity of DNA by copper(II)/iron(III)-adriamycin in the presence of ascorbate and hydrogen peroxide. SECCA, a succinylated derivative of coumarin, generates the fluorescent 7-hydroxy-SECCA following reaction with HO.. SECCA was coupled to polylysine or to histone H1 and then …
  • Age-dependent prolactin-releasing activity of nucleoproteins. [Journal Article]
    Mech Ageing Dev. 1996 Aug 15; 89(2):103-11.Brown OA, Sosa YE, … Goya RG
  • In previous studies we demonstrated that histone preparations possess multiple effects in vivo on pituitary hormone secretion and that these effects tend to disappear with age. We have now evaluated the in vitro effects of histone and nucleohistone preparations on the secretion of prolactin (PRL) in perifused pituitary cells from young (4 months) and senescent (29-33 months) female rats. Freshly …
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