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(optimal cutting temperature compound)
32 results
  • Correlative light and immuno-electron microscopy of retinal tissue cryostat sections. [Journal Article]
    PLoS One 2018; 13(1):e0191048Burgoyne T, Lane A, … Futter CE
  • Correlative light-electron microscopy (CLEM) is a powerful technique allowing localisation of specific macromolecules within fluorescence microscopy (FM) images to be mapped onto corresponding high-resolution electron microscopy (EM) images. Existing methods are applicable to limited sample types and are technically challenging. Here we describe novel methods to perform CLEM and immuno-electron m…
  • Preservation of three-dimensional spatial structure in the gut microbiome. [Journal Article]
    PLoS One 2017; 12(11):e0188257Hasegawa Y, Mark Welch JL, … Borisy GG
  • Preservation of three-dimensional structure in the gut is necessary in order to analyze the spatial organization of the gut microbiota and gut luminal contents. In this study, we evaluated preparation methods for mouse gut with the goal of preserving micron-scale spatial structure while performing fluorescence imaging assays. Our evaluation of embedding methods showed that commonly used media suc…
  • Precast Gelatin-Based Molds for Tissue Embedding Compatible with Mass Spectrometry Imaging. [Journal Article]
    Anal Chem 2017; 89(1):576-580Gill EL, Yost RA, … Garrett TJ
  • Preparation of tissue for matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) generally involves embedding the tissue followed by freezing and cryosectioning, usually between 5 and 25 μm thick, depending on the tissue type and the analyte(s) of interest. The brain is approximately 60% fat; it therefore lacks rigidity and poses structural preservation challenges durin…
  • Quantification of Colonic Stem Cell Mutations. [Journal Article]
    J Vis Exp 2015; (103)Whetstone RD, Gold B
  • The ability to measure stem cell mutations is a powerful tool to quantify in a critical cell population if, and to what extent, a chemical can induce mutations that potentially lead to cancer. The use of an enzymatic assay to quantify stem cell mutations in the X-linked glucose-6-phosphate dehydrogenase gene has been previously reported.(1) This method requires the preparation of frozen sections …
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