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Unbound Medicine.
(optimal cutting temperature compound)
37 results
  • A simple method for sphingolipid analysis of tissues embedded in optimal cutting temperature compound. [Journal Article]
    J Lipid Res. 2020 Jun; 61(6):953-967.Rohrbach TD, Boyd AE, … Lima S
  • MS-assisted lipidomic tissue analysis is a valuable tool to assess sphingolipid metabolism dysfunction in disease. These analyses can reveal potential pharmacological targets or direct mechanistic studies to better understand the molecular underpinnings and influence of sphingolipid metabolism alterations on disease etiology. But procuring sufficient human tissues for adequately powered studies c…
  • Fast Localization and Sectioning of Mouse Locus Coeruleus. [Journal Article]
    Biomed Res Int. 2020; 2020:4860735.Cao S, Li J, … Yu T
  • The locus nucleus (LC) is a multifunctional nucleus which is also the source of norepinephrine in the brain. To date, there is no simple and easy method to locate the small LC in brain sectioning. Here we report a fast, accurate, and easy-to-follow protocol for the localization of mice LC in frozen sectioning. After fixation and dehydration, the intact brains of adult mice were placed on a horizo…
  • Cryo-Gel embedding compound for renal biopsy biobanking. [Journal Article]
    Sci Rep. 2019 Oct 24; 9(1):15250.Snijders MLH, Zajec M, … Clahsen-van Groningen MC
  • Optimal preservation and biobanking of renal tissue is vital for good diagnostics and subsequent research. Optimal cutting temperature (OCT) compound is a commonly used embedding medium for freezing tissue samples. However, due to interfering polymers in OCT, analysis as mass spectrometry (MS) is difficult. We investigated if the replacement of OCT with Cryo-Gel as embedding compound for renal bi…
  • Correlative light and immuno-electron microscopy of retinal tissue cryostat sections. [Journal Article]
    PLoS One. 2018; 13(1):e0191048.Burgoyne T, Lane A, … Futter CE
  • Correlative light-electron microscopy (CLEM) is a powerful technique allowing localisation of specific macromolecules within fluorescence microscopy (FM) images to be mapped onto corresponding high-resolution electron microscopy (EM) images. Existing methods are applicable to limited sample types and are technically challenging. Here we describe novel methods to perform CLEM and immuno-electron m…
  • Preservation of three-dimensional spatial structure in the gut microbiome. [Journal Article]
    PLoS One. 2017; 12(11):e0188257.Hasegawa Y, Mark Welch JL, … Borisy GG
  • Preservation of three-dimensional structure in the gut is necessary in order to analyze the spatial organization of the gut microbiota and gut luminal contents. In this study, we evaluated preparation methods for mouse gut with the goal of preserving micron-scale spatial structure while performing fluorescence imaging assays. Our evaluation of embedding methods showed that commonly used media suc…
  • Precast Gelatin-Based Molds for Tissue Embedding Compatible with Mass Spectrometry Imaging. [Journal Article]
    Anal Chem. 2017 01 03; 89(1):576-580.Gill EL, Yost RA, … Garrett TJ
  • Preparation of tissue for matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) generally involves embedding the tissue followed by freezing and cryosectioning, usually between 5 and 25 μm thick, depending on the tissue type and the analyte(s) of interest. The brain is approximately 60% fat; it therefore lacks rigidity and poses structural preservation challenges durin…
  • Quantification of Colonic Stem Cell Mutations. [Journal Article]
    J Vis Exp. 2015 Sep 25Whetstone RD, Gold B
  • The ability to measure stem cell mutations is a powerful tool to quantify in a critical cell population if, and to what extent, a chemical can induce mutations that potentially lead to cancer. The use of an enzymatic assay to quantify stem cell mutations in the X-linked glucose-6-phosphate dehydrogenase gene has been previously reported.(1) This method requires the preparation of frozen sections …
  • Ursolic acid improves domoic acid-induced cognitive deficits in mice. [Journal Article]
    Toxicol Appl Pharmacol. 2013 Sep 01; 271(2):127-36.Wu DM, Lu J, … Li MQ
  • Our previous findings suggest that mitochondrial dysfunction is the mechanism underlying cognitive deficits induced by domoic acid (DA). Ursolic acid (UA), a natural triterpenoid compound, possesses many important biological functions. Evidence shows that UA can activate PI3K/Akt signaling and suppress Forkhead box protein O1 (FoxO1) activity. FoxO1 is an important regulator of mitochondrial func…
  • Immunofluorescence staining with frozen mouse or chick embryonic tissue sections. [Journal Article]
    Methods Mol Biol. 2013; 1018:175-88.Wang H, Matise MP
  • Immunofluorescence (IF), a form of immunohistochemistry (IHC) with specific applications, is commonly used for both basic research and clinical studies, including diagnostics, and involves visualizing the cellular distribution of target molecules (e.g., proteins, DNA, and small molecules) using a microscope capable of exciting and detecting fluorochrome compounds that emit light at specific, larg…
  • Cryosectioning tissues. [Journal Article]
    CSH Protoc. 2008 Aug 01; 2008:pdb.prot4991.Fischer AH, Jacobson KA, … Zeller R
  • INTRODUCTION Cryosections are rapidly and relatively easily prepared prior to fixation, and they provide a good system for visualizing fine details of the cell. Although cryosections are physically less stable than paraffin- or resin-embedded sections, they are generally superior for the preservation of antigenicity and therefore the detection of antigens by microscopy. The preparation of cryosec…
  • A novel method for freezing and storing research tissue bank specimens. [Journal Article]
    Hum Pathol. 2005 Sep; 36(9):977-80.Loken SD, Demetrick DJ
  • Preserving small pieces of frozen tissue for possible future ancillary studies ("tumor banking") usually involves either placing a small piece of tissue in a cryovial and snap freezing it in liquid nitrogen or embedding and freezing the tissue in a block of cryopreservation medium, such as optimal cutting temperature (OCT) compound. The cryovial storage method leaves an irregularly shaped piece o…
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