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4,724 results
  • The GID ubiquitin ligase complex is a regulator of AMPK activity and organismal lifespan. [Journal Article]
    Autophagy 2019; :1-17Liu H, Ding J, … Pfirrmann T
  • The AMP-activated protein kinase (AMPK) regulates cellular energy homeostasis by sensing the metabolic status of the cell. AMPK is regulated by phosphorylation and dephosphorylation as a result of changing AMP/ATP levels and by removal of inhibitory ubiquitin residues by USP10. In this context, we identified the GID-complex, an evolutionarily conserved ubiquitin-ligase-complex (E3), as a negative…
  • Methods for successful inactivation of Rift Valley fever virus in infected mosquitoes. [Journal Article]
    J Virol Methods 2019; :113794Bergren NA, Patterson EI, … Kading RC
  • Ensuring the successful inactivation of select agent material is critical for maintaining compliance with federal regulations and safeguarding laboratory personnel from exposure to dangerous pathogens. Rift Valley fever virus (RVFV), naturally transmitted by mosquitoes, is classified as a select agent by the CDC and USDA due to its potential to cause significant economic losses to the livestock i…
  • Human MAIT Cell Activation In Vitro. [Journal Article]
    Methods Mol Biol 2020; 2098:97-124Hagel JP, Garner LC, … Klenerman P
  • Mucosal-associated invariant T (MAIT) cells are an abundant innate-like T cell subset in humans, enriched in mucosal tissues and the liver. MAIT cells express a semi-invariant T cell receptor (TCR) and recognize microbial-derived riboflavin metabolites presented on the MHC Class I-like molecule MR1. In addition to activation via the TCR, MAIT cells can also be activated in response to cytokines s…
  • Effective methods for the inactivation of Francisella tularensis. [Journal Article]
    PLoS One 2019; 14(11):e0225177Azaki M, Uda A, … Morikawa S
  • Francisella tularensis (F. tularensis) is highly pathogenic to humans and must be handled under biosafety level 3 conditions. Samples used for the diagnosis and experimental analysis must be completely inactivated, although methods for the inactivation of F. tularensis are limited. In this study, effective methods for the inactivation of F. tularensis SCHU P9 and five other strains were determine…
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