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28 results
  • An alternative method for Plasmodium culture synchronization. [Journal Article]
    Exp Parasitol. 2005 Mar; 109(3):195-7.Lelièvre J, Berry A, Benoit-Vical F
  • Since the synchronization of Plasmodium falciparum has become an essential tool in research, we have investigated the use of a commercial gelatine solution, Plasmion, to replace Plasmagel, which is now difficult to obtain. This method also avoids the use of techniques based on Percoll-glucose gradients. The Plasmion-based technique proved to be a good method and could become an alternative to Pla…
  • Different Babesia canis isolates, different diseases. [Journal Article]
    Parasitology. 1997 Nov; 115 (Pt 5):485-93.Schetters TP, Moubri K, … Gorenflot A
  • Using surface immunofluorescence isolate-specific antigens were detected on the membrane of erythrocytes infected with Babesia parasites. In addition, the strains reacted differently with Plasmagel in that the European isolate (B.c. canis) could be purified on Plasmagel effectively, whereas infected erythrocytes of the South-African isolate (B.c. rossi) could not. Experimental infection of dogs w…
  • The production of collagenase by adherent mononuclear cells cultured from human peripheral blood. [Journal Article]
    Arthritis Rheum. 1984 Dec; 27(12):1397-404.Louie JS, Weiss J, … Uitto J
  • Mononuclear cells were isolated from human peripheral blood by Ficoll-Hypaque centrifugation, and the cells adherent to plastic substrata were cultured in serum-free media supplemented with lactalbumin hydrolysate. These cell cultures, which consisted predominantly of monocyte-macrophages as judged by nonspecific esterase staining, accumulated collagenase in the medium. This collagenase resembled…
  • Morphological aspects of the rat kidney preserved by cold storage. III. Ultrastructural aspects. [Journal Article]
    Morphol Embryol (Bucur). 1983 Oct-Dec; 29(4):279-82.Neagu S, Gabrielescu E, … Chirculescu AR
  • The ultrastructural changes of the white Wistar rat kidney, preserved by cold storage into two preservation media (Sacks and Plasmagel) are reported. For each medium two groups of kidney were used; for one of them the medium was used without additives and for the other stabilizing additives were added. The results showed the favourable effects of additives on the maintenance of the cellular ultra…
  • Morphological aspects of the rat kidney preserved by cold storage. I. Glomerular morphometric changes. [Journal Article]
    Morphol Embryol (Bucur). 1983 Apr-Jun; 29(2):121-5.Neagu S, Chirculescu AR, … Popescu F
  • The absolute density of glomeruli in the microscopic field was determined in the rat kidney preserved by cold storage for 24, 48, 72 and 96 hrs in two different media: Sacks (hyperosmolar electrolytic solution of intracellular type) and Plasmagel (gelatin solution 4%). Progressive, statistically significant (p less than 0.01) decrease of glomerular density at 24 and 48 hrs was followed by return …
  • [Anaphylactic reactions to modified fluid gelatins. (A case of allergy to Plasmagel)]. [Case Reports]
    Ann Anesthesiol Fr. 1980; 21(2):148-52.Saissy JM, Legoury O, … Vilette B
  • A case of anaphylactic reaction to an infusion of modified fluid gelatin (Plasmagel) which occurred just before the induction of anaesthesia is reported. The allergic origin of this side effect was sought by a human basophil degranulation test (Benveniste test) which proved to be positive. Intolerance reactions to gelatins have up to now been little described in the French literature (in contrast…
  • Harvest of Plasmodium falciparum merozoites from continuous culture. [Journal Article]
    Bull World Health Organ. 1979; 57 Suppl 1:63-8.Mrema JE, Campbell GH, … Rieckmann KH
  • Spontaneously released merozoites were harvested from cultures in which 42-90% of the erythrocytes had been infected with mature forms of Plasmodium falciparum at the start of incubation. The mature forms had been extracted from asynchronous cultures by the use of Ficoll and Plasmagel gradients. As the mature forms consisted of both trophozoites and schizonts, merozoites were released into the cu…
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