- Marinicauda salina sp. nov., isolated from a marine solar saltern. [Journal Article]
- IJInt J Syst Evol Microbiol 2019 Jun 14
- A novel Gram-stain-negative, aerobic, oxidase-positive, motile, dimorphic rod bacterium, with a polar flagellum or a polar prostheca, designated as strain WD6-1T, was isolated from a sediment sample …
A novel Gram-stain-negative, aerobic, oxidase-positive, motile, dimorphic rod bacterium, with a polar flagellum or a polar prostheca, designated as strain WD6-1T, was isolated from a sediment sample collected from a marine solar saltern located in Weihai, PR China. Growth of strain WD6-1T was observed at 15-45 °C (optimum, 37-40 °C). The pH range for growth was pH 6.0-9.5 (optimum, pH 7.0-7.5) while the NaCl concentration was 1.0-16.0 % (w/v; optimum, 5.0 %). The most closely related species was Marinicauda algicola (97.0 % 16S rRNA gene sequence similarity). The DNA G+C content of strain WD6-1T was 69.5 mol% and the sole respiratory quinone was ubiquinone 10 (Q-10). The major cellular fatty acids (>10 %) of strain WD6-1T included summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C18 : 0 and C17 : 0, and the major polar lipids were glucuronopyranosyldiglyceride, monoglycosyldiglyceride and sulfo-quinovosyl diacylglycerol. Based on the results of phylogenetic, genotypic and phenotypic analyses, the isolate is representative of a new member of the genus Marinicauda, for which the name Marinicaudasalina sp. nov. is proposed. The type strain is WD6-1T (=KCTC 62348T=MCCC 1H00282T).
- A Comparative Evaluation of Five Obturation Techniques in the Management of Simulated Internal Resorptive Cavities: An Ex Vivo Study. [Journal Article]
- JPJ Pharm Bioallied Sci 2019; 11(Suppl 2):S450-S456
- CONCLUSIONS: It can be concluded that Obtura II along with System B was found to be the most suitable obturation technique for the management of teeth exhibiting internal resorption. Thermafil was found to give the poorest obturation quality when used to fill the teeth with internal resorption. Similarly, lateral condensation technique was observed to show maximum sealer and hence was not ideal for the management of internal resorptive cavities.
- Adjuvant Chemotherapy plus Radiation for Locally Advanced Endometrial Cancer. [Journal Article]
- NEJMN Engl J Med 2019 06 13; 380(24):2317-2326
- CONCLUSIONS: Chemotherapy plus radiation was not associated with longer relapse-free survival than chemotherapy alone in patients with stage III or IVA endometrial carcinoma. (Funded by the National Cancer Institute; ClinicalTrials.gov number, NCT00942357.).
- Fulvimarina endophytica sp. nov., a novel endophytic bacterium isolated from bark of Sonneratia caseolaris. [Journal Article]
- JMJ Microbiol 2019 Jun 11
- A Gram-negative, aerobic, short-rod-shaped, motile (with a terminal flagellum), non-spore-forming bacterium, designated strain 85T, was isolated from a surface-sterilized bark of Sonneratia caseolari…
A Gram-negative, aerobic, short-rod-shaped, motile (with a terminal flagellum), non-spore-forming bacterium, designated strain 85T, was isolated from a surface-sterilized bark of Sonneratia caseolaris collected from Qinzhou in Guangxi, China and was analyzed using a polyphasic approach to determine its taxonomic position. Strain 85T grew optimally in the presence of 1-2% (w/v) NaCl at 30°C and pH 6.0-7.0. Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 85T belonged to the genus Fulvimarina and shared the highest 16S rRNA gene sequence similarity with Fulvimarina pelagi HTCC2506T (96.16%). The cell-wall peptidoglycan contained meso-diaminopimelic acid and ubiquinone Q-10 was the predominant respiratory lipoquinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified amino lipid, three unidentified phospholipids and six unidentified lipids. The major fatty acid was C18:1ω7c. The DNA G+C content of strain 85T was 65.4 mol%, and the average nucleotide identity and estimated DDH values between strain 85T and the type strain of Fulvimarina pelagi HTCC2506T were 77.3% and 21.7%, respectively. Based on the phylogenetic, phenotypic, and chemotaxonomic analyses, strain 85T should be considered as a novel species of the genus Fulvimarina with the proposed name Fulvimarina endophytica sp. nov., and its type strain is 85T (= KCTC 62717T = CGMCC 1.13665T).
- Conventional and molecular cytogenetic studies to characterize 32 complex variant Philadelphia translocations in patients with chronic myeloid leukemia. [Journal Article]
- OLOncol Lett 2019; 17(6):5705-5710
- BCR/ABL1 gene fusion is the hallmark of chronic myeloid leukemia (CML), and is generated in 5-10% of patients by a variant translocation involving 9q34, 22q11.2 and one or more additional genomic reg…
BCR/ABL1 gene fusion is the hallmark of chronic myeloid leukemia (CML), and is generated in 5-10% of patients by a variant translocation involving 9q34, 22q11.2 and one or more additional genomic regions. The objective of the present study was to characterize, by conventional and molecular cytogenetics, 32 complex variant Philadelphia (Ph) translocations present at diagnosis in patients with CML. The chromosomes most frequently involved were 1 and 5, and the breakpoint most frequently involved was 12p13. The q-chromosome arm was more frequently involved (60%) than the p-arm. The breakpoints were located in the G-light bands in the majority of cases (85%). Additional chromosomal abnormalities were observed in 6 out of 32 (19%) patients. In conclusion, the combination of conventional and molecular cytogenetics studies has allowed us to: i) Detect and quantify the BCR/ABL1 fusion gene; ii) characterize the complex variant translocations and detect cryptic translocations; iii) confirm that the breakpoints are commonly localized in the G-light bands; (iv) confirm that the genesis of variant translocations could be via either the one-step or two-step mechanisms; and v) to report new cases of complex variant translocations.
- The HEXACO-100 Across 16 Languages: A Large-Scale Test of Measurement Invariance. [Journal Article]
- JPJ Pers Assess 2019 Jun 11; :1-13
- The HEXACO Personality Inventory-Revised (HEXACO-PI-R) has become one of the most heavily applied measurement tools for the assessment of basic personality traits. Correspondingly, the inventory has …
The HEXACO Personality Inventory-Revised (HEXACO-PI-R) has become one of the most heavily applied measurement tools for the assessment of basic personality traits. Correspondingly, the inventory has been translated to many languages for use in cross-cultural research. However, formal tests examining whether the different language versions of the HEXACO-PI-R provide equivalent measures of the 6 personality dimensions are missing. We provide a large-scale test of measurement invariance of the 100-item version of the HEXACO-PI-R across 16 languages spoken in European and Asian countries (N = 30,484). Multigroup exploratory structural equation modeling and confirmatory factor analyses revealed consistent support for configural and metric invariance, thus implying that the factor structure of the HEXACO dimensions as well as the meaning of the latent HEXACO factors is comparable across languages. However, analyses did not show overall support for scalar invariance; that is, equivalence of facet intercepts. A complementary alignment analysis supported this pattern, but also revealed substantial heterogeneity in the level of (non)invariance across facets and factors. Overall, results imply that the HEXACO-PI-R provides largely comparable measurement of the HEXACO dimensions, although the lack of scalar invariance highlights the necessity for future research clarifying the interpretation of mean-level trait differences across countries.
- Hydrocarboniclastica marina gen. nov., sp. nov., a marine hydrocarbonoclastic bacterium isolated from an in situ enriched hydrocarbon-degrading consortium in sea sediment. [Journal Article]
- IJInt J Syst Evol Microbiol 2019 Jun 11
- A Gram-stain-negative, motile, non-spore-forming, aerobic and rod-shaped bacterial strain, Soil36-7T, was isolated from an in situ enriched hydrocarbon-degrading consortium in South China Sea sedimen…
A Gram-stain-negative, motile, non-spore-forming, aerobic and rod-shaped bacterial strain, Soil36-7T, was isolated from an in situ enriched hydrocarbon-degrading consortium in South China Sea sediment. Strain Soil36-7T grew at 4-40 °C (optimum 28-32 °C), at pH 5-10 (pH 7-8) and in the presence of 1-12 % (w/v) NaCl (3-6 %). Phylogenetic analyses based on 16S rRNA gene sequences and a genome-based approach using UBCGs (up-to-date bacterial core genes) showed Soil36-7T formed a distinct branching lineage within the family Alteromonadaceae. 16S rRNA gene sequence similarity was 92.9, 92.1 and >88.3 % between strain Soil36-7T and the type species of the genera Marinobacter, Tamilnaduibacter and the other genera of the family Alteromonadaceae, respectively. The major fatty acids in Soil36-7T were C16 : 0, C16 : 1ω6/7c, C16 : 0 10-methyl, C18 : 1ω7c, C12 : 0 and C18 : 0. The predominant respiratory quinone was Q-9, with a minor amount of Q-10 (3.5 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and various unidentified glycolipids, phospholipids, aminophospholipids and other polar lipids. The DNA G+C content was 57.9 mol%. On the basis of phylogenetic, genomic, phenotypic and chemotaxanomic characteristics, strain Soil36-7T could be classified as representing a novel species of a new genus within the family Alteromonadaceae, for which the name Hydrocarboniclastica marina gen. nov., sp. nov. is proposed. The type strain of the type species is Soil36-7T (=MCCC 1A12105T=KCTC 62334T).
- The relationship between sleep quality and all-cause, CVD and cancer mortality: the Southall and Brent REvisited study (SABRE). [Journal Article]
- SMSleep Med 2019 Mar 27
- CONCLUSIONS: Our findings suggest that the relationship between sleep quality and mortality may differ by ethnic group, but formal heterogeneity tests indicated that the strongest difference in HRs was observed for early morning waking and cardiovascular disease (CVD) mortality across the three groups (Cochran's Q test p = 0.036). As such, these results are novel and provide support for ethnic differences in sleep quality and mortality, and may have implications for precision medicine.
- Comparison of the Kane formula with existing formulas for intraocular lens power selection. [Journal Article]
- BOBMJ Open Ophthalmol 2019; 4(1):e000251
- CONCLUSIONS: Using three variables (AL, K, ACD), the Kane formula was a more accurate predictor of actual postoperative refraction than the other formulae under investigation. There were not enough eyes of short or long axial length to adequately power statistical comparisons within axial length subgroups.
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- Vibrio profundi sp. nov., isolated from a deep-sea seamount. [Journal Article]
- AVAntonie Van Leeuwenhoek 2019 Jun 06
- A Gram-stain negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP187T, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic an…
A Gram-stain negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP187T, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain TP187T is related to members of the genus Vibrio and has high 16S rRNA gene sequence similarity with the type strains of Vibrio chagasii (97.3%) and Vibrio gallaecicus (97.1%). Sequence similarities to all other type strains of current species of the genus Vibrio were below 97%. The polar lipids profile was found to contain diphosphatidylglycerol, phosphatidylglycerol, an aminophospholipid, two aminolipids, four phospholipids and eleven unidentified polar lipids. Ubiquinone Q-8 was detected as the predominant quinone. The genomic DNA G + C content of strain TP187T was determined to be 43.7 mol%. In addition, the maximum values of in silico DNA-DNA hybridization (isDDH) and average nucleotide identity (ANI) between strain TP187T with V. chagasii LMG 21353T were 22.40 and 77.50% respectively. Both values are below the proposed cutoff levels for species delineation, i.e. 70 and 95%, respectively. Combined data from phenotypic, phylogenetic, isDDH and ANI data demonstrated that the strain TP187T is representative of a novel species of the genus Vibrio, for which we propose the name Vibrio profundi sp. nov. (type strain TP187T = KACC 18555T = CGMCC 1.15395T).