- Comparison of DNA Extraction Efficiency and Reproducibility of Different Aeration Diffuser Biofilms Using Bead-Beating Protocol. [Journal Article]
- JMJ Mol Microbiol Biotechnol 2019 Jun 24; 28(6):293-303
- An existing bead-beating DNA extraction protocol was employed to compare the DNA extraction recovery and fragment quality of 6 different aeration diffuser biofilms. Escherichia coli, Gordonia amarae,…
An existing bead-beating DNA extraction protocol was employed to compare the DNA extraction recovery and fragment quality of 6 different aeration diffuser biofilms. Escherichia coli, Gordonia amarae, and mixed liquor were used as controls. The fraction of total DNAbiofilm decreased monotonically with increasing number of beat beatings (BB) when the amount of DNA present was sufficient (>4 μgDNA/cm2), excluding the ceramic disk. While controls required only 2 BBs, 3 out of 5 BBs achieved ≥70% of total DNA (70.3 ± 1.7%) for 5 out of 6 biofilms. Quantitative polymerase chain reaction (PCR) analyses of 353 and 1,505 basepair (bp) amplicons from pure culture extracts showed target copy numbers were not degraded for the first 2 BBs, but the third BB decreased amplicon concentrations by 0.65 and 1.12 log for E. coli, and 0.39 and 0.40 log for G. amarae, respectively. The 353 bp fragment amplification from biofilm samples showed minimal degradation for the first 3 BBs. PCR and gel electrophoresis confirmed integrity of amplified 1,505 bp DNA fragments over the 5 BBs, except in the EDPM (75 mm diameter, tube) diffuser biofilm (4.98 ± 0.62 μgDNA/cm2). Taken together, this study showed type of diffuser membrane biofilms had no effects on extraction efficiency, but low DNA concentrations reduced extraction performance.
- Components of community rehabilitation programme for adults with chronic conditions: A systematic review. [Review]
- IJInt J Nurs Stud 2019 May 31; 97:114-129
- CONCLUSIONS: The findings suggest community rehabilitation programmes for individuals with chronic conditions be a minimum of 4-6 weeks to cover necessary education for management of symptoms, be led by a health professional/s in combination with lay leaders, and include development of self-management skills. We recommend consideration be given to health literacy level of the programme, and that because of the known positive benefit of exercise on physical functioning, quality of life and in slowing progression of chronic conditions, an exercise time should be included. Lastly, we recommend that reporting and interpreting effect sizes of interventions within studies would facilitate more useful choice of outcome measures to be able to demonstrate clinically meaningful change.
- A complex urban ecological investigation in a mid-sized Hungarian city - SITE assessment and monitoring of a liveable urban area, PART 1: Water quality measurement. [Journal Article]
- JEJ Environ Manage 2019 Jun 21; 247:78-87
- Urbanization and related environmental pollution have strong effects on stream systems by inducing short duration high-peak floods, raised levels of nutrients and contaminants, altered channel geomor…
Urbanization and related environmental pollution have strong effects on stream systems by inducing short duration high-peak floods, raised levels of nutrients and contaminants, altered channel geomorphology, sediment dynamics, and reduced biotic richness. The main purpose of this current study is to detect stream contamination levels in a mid-sized Hungarian city by comparing the results of two separate years (2011, 2018). Discharge, channel geomorphology, and water quality parameters were measured, and load-based contamination was calculated for the city's main watercourse (Gaja Brook). The pH, CaCO3, texture, and heavy metal contents - Cd, Co, Cu, Ni, Pb, and Zn - were reanalysed in the sediment for both investigated years. For comparison, enrichment factors were used to determine sediment accumulation. As a complex parameter, biological water quality was also determined using BMWP and MMCP protocols in 2018. The results show that conductivity and the nutrient concentrations were higher, but the discharge values were lower in 2011 than in 2018. The nitrate load doubled both times in the brook between the first and the last sampling sites. The enrichment factors decreased or stagnated when the values of the two years were compared, but severe enrichment of Cd was detected in the middle of the city and south of the city. The aquatic macroinvertebrate fauna structure defined clean, but slightly impacted watercourses north and south of the city as well, but the abundance and presence of sensitive taxa differed at the sampling sites. Székesfehérvár has better than expected water quality, which can be attributed to the good ecological states of the hydromorphology and the streamside zone.
- Dyspnea in amyotrophic lateral sclerosis: The Dyspnea-ALS-Scale (DALS-15) essentially contributes to the diagnosis of respiratory impairment. [Journal Article]
- RMRespir Med 2019 Jun 19; 154:116-121
- CONCLUSIONS: Our study clearly shows that dyspnea can occur independently of objective indicators of respiratory impairment like spirometry or blood gases. Hence, the DALS-15 covers another aspect of respiratory impairment than these tests and refers to the subjective component of respiratory impairment. It detects dyspnea in a considerable proportion of patients in whom NIV should thus be considered although their spirometric and blood gas results do not point towards NIV. The DALS-15 therefore may help to improve the stratification of patients with respiratory impairment for more efficient symptom management and timely coordination of care.
- Seasonality effects on consumers' preferences over quality attributes of different beef products. [Journal Article]
- MSMeat Sci 2019 Jun 14; 157:107868
- Using discrete choice modelling, the study investigates 946 American consumers' willingness-to-pay and preferences for diverse beef products. A novel experiment was used to elicit the number of beef …
Using discrete choice modelling, the study investigates 946 American consumers' willingness-to-pay and preferences for diverse beef products. A novel experiment was used to elicit the number of beef products that each consumer would purchase. The range of products explored in this study included ground, diced, roast, and six cuts of steaks (sirloin, tenderloin, flank, flap, New York and cowboy/rib-eye). The outcome of the study suggests that US consumers vary in their preferences for beef products by season. The presence of a USDA certification logo is by far the most important factor affecting consumer's willingness to pay for all beef cuts, which is also heavily dependent on season. In relation to packaging, US consumers have mixed preference for different beef products by season. The results from a scaled adjusted ordered logit model showed that after price, safety-related attributes such as certification logos, types of packaging, and antibiotic free and organic products are a stronger influence on American consumers' choice. Furthermore, US consumers on average purchase diced and roast products more often in winter "slow cooking season", than in summer; whereas New York strip and flank steak are more popular in the summer "grilling season". This study provides valuable insights for businesses as well as policymakers to make inform decisions while considering how consumers relatively value among different labelling and product attributes by season and better address any ethical, safety and aesthetic concerns that consumers might have.
- Atmospheric nitrogen pollution in urban agglomeration and its impact on alpine lake-case study of Tianchi Lake. [Journal Article]
- STSci Total Environ 2019 Jun 14; 688:312-323
- The atmospheric pollution caused by human activities has been recognized as an important factor affecting the water quality of freshwater bodies. The process of the human factors' impact on high-alti…
The atmospheric pollution caused by human activities has been recognized as an important factor affecting the water quality of freshwater bodies. The process of the human factors' impact on high-altitude lakes in inland regions is not clear up to now. In this research, regions around Tianchi Lake were taken as a case study to explore the relation between the urban air pollution and alpine lake water quality. Multi-scale station observed data were analyzed for the urban NO2 pollution by means of relevance analysis and trend analysis, the field measured data were analyzed for the lake total nitrogen (TN) pollution using multiple methods including the water quantity and quality balance, remote sensing retrieval and nutrient load assessment. The sources and occurrence conditions of atmospheric pollution and lake pollution were identified by a multi-method driving factor analysis. As a result, there is sufficient direct and indirect evidence to prove that the serious air pollution in surrounding cities is an important cause of the nitrogen pollution in Tianchi Lake.
- Gaucher Disease in Bone: From Pathophysiology to Practice. [Review]
- JBJ Bone Miner Res 2019 Jun 24
- Gaucher disease (GD) is a rare, genetic lysosomal disorder leading to lipid accumulation and dysfunction in multiple organs. Involvement of the skeleton is one of the most prevalent aspects of GD and…
Gaucher disease (GD) is a rare, genetic lysosomal disorder leading to lipid accumulation and dysfunction in multiple organs. Involvement of the skeleton is one of the most prevalent aspects of GD and a major cause of pain, disability, and reduced quality of life. Uniform recommendations for contemporary evaluation and management are needed. To develop practical clinical recommendations, an international group of experienced physicians conducted a comprehensive review of 20 years' of the literature, defining terms according to pathophysiological understanding and pointing out best practice and unmet needs related to the skeletal features of this disorder. Abnormalities of bone modeling, reduced bone density, bone infarction, and plasma cell dyscrasias accompany the displacement of healthy adipocytes in adult marrow. Exposure to excess bioactive glycosphingolipids appears to affect hematopoiesis and the balance of osteoblast and osteoclast numbers and activity. Imbalance between bone formation and breakdown induces disordered trabecular and cortical bone modeling, cortical bone thinning, fragility fractures, and osteolytic lesions. Regular assessment of bone mineral density, marrow infiltration, the axial skeleton and searching for potential malignancy are recommended. MRI is valuable for monitoring skeletal involvement: It provides semiquantitative assessment of marrow infiltration and the degree of bone infarction. When MRI is not available, monitoring of painful acute bone crises and osteonecrosis by plain X-ray has limited value. In adult patients, we recommend DXA of the lumbar spine and left and right hips, with careful protocols designed to exclude focal disease; serial follow-up should be done using the same standardized instrument. Skeletal health may be improved by common measures, including adequate calcium and vitamin D and management of pain and orthopedic complications. Prompt initiation of specific therapy for GD is crucial to optimizing outcomes and preventing irreversible skeletal complications. Investing in safe, clinically useful, and better predictive methods for determining bone integrity and fracture risk remains a need. © 2019 The Authors. Journal of Bone and Mineral Research Published by Wiley Periodicals Inc.
- Beyond medical therapies-clinicians' knowledge of cosmetic products in managing individuals with hair loss. [Letter]
- CEClin Exp Dermatol 2019 Jun 24
- Alopecia is one of the most distressing conditions with significant negative impact on quality of life, social interaction and even performance in the workplace.(1) Medical management utilises anti-i…
Alopecia is one of the most distressing conditions with significant negative impact on quality of life, social interaction and even performance in the workplace.(1) Medical management utilises anti-inflammatory agents, antiandrogens or immunosuppressants alongside hair growth stimulants and medicated shampoos(2) which take as a minimum of 6-9 months to result in visible improvement and may have undesirable side-effects. This article is protected by copyright. All rights reserved.
- Characteristics of nursing interventions that improve the quality of life of people with chronic diseases. A systematic review with meta-analysis. [Journal Article]
- PlosPLoS One 2019; 14(6):e0218903
- CONCLUSIONS: Interventions targeting people with chronic diseases resulted in a slight increase in the HRQoL that was not always significant, which suggests that there is a need for their continuous improvement.
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- Follicular lymphomas in vulnerable/older patients. [Journal Article]
- COCurr Opin Oncol 2019 Jun 20
- CONCLUSIONS: Given the lack of prospective studies there is an urgent need to investigate if geriatric assessment including comorbidities, geriatric parameters, patient's reported outcomes and quality of life issues, may help selecting those frail elderly follicular lymphoma patients less suitable for full-dose treatments. In addition, the potential usefulness of the new noncytotoxic agents of proven efficacy in follicular lymphoma warrants specific investigations in older patients.