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Unbound Medicine.
(radioimmunoelectrophoresis)
250 results
  • Cross-reactivity between storage and dust mites and between mites and shrimp. [Journal Article]
    Exp Appl Acarol. 2009 Feb; 47(2):159-72.Arlian LG, Morgan MS, … Sharra D
  • Many patients have sensitivities to multiple species of storage and house dust mites. It is not clear if this is because patients have multiple sensitivities to species-specific mite allergens or if these mites share many cross-reacting allergens. Our objective was to further define the cross-allergenicity between several species of storage and house dust mites using crossed-immunoelectrophoresis…
  • Characterization of allergens of Anisakis simplex. [Journal Article]
    Allergy. 2003 Dec; 58(12):1299-303.Arlian LG, Morgan MS, … Fernández-Caldas E
  • CONCLUSIONS: Somatic extracts of A. simplex L3 larva contain a large number of allergenic molecules and there is significant variability between patients in their sensitivity and reactivity to these allergens.
  • Allergenicity of the mite Hemisarcoptes cooremani. [Journal Article]
    Ann Allergy Asthma Immunol. 1999 Dec; 83(6 Pt 1):529-32.Arlian LG, Morgan MS, Houck MA
  • CONCLUSIONS: These results indicated that an extract of the mite H. cooremani contained at least two prominent IgE binding proteins that were not present in the other astigmatid mites. Thus, H. cooremani is the source of unique allergenic proteins and allergy to this mite may develop in orchard and ornamental nursery workers and gardeners.
  • Cow's milk casein, a hidden allergen in natural rubber latex gloves. [Journal Article]
    J Allergy Clin Immunol. 1999 Jul; 104(1):177-80.Ylitalo L, Mäkinen-Kiljunen S, … Reunala T
  • CONCLUSIONS: Several brands of NRL gloves were found to contain casein, implying that extracts prepared from such gloves can cause false-positive skin prick test reactions when diagnosing NRL allergy. The use of casein as a stabilizer in glove manufacture without appropriate labeling should be stopped because it can also cause contact urticaria syndrome in individuals with cow's milk allergy.
  • Allergenic characterization of Tyrophagus putrescentiae using sera from occupationally exposed farmers. [Journal Article]
    Ann Allergy Asthma Immunol. 1997 Dec; 79(6):525-9.Arlian LG, Vyszenski-Moher DL, … van Hage-Hamsten M
  • CONCLUSIONS: The results of this study showed that farmers who were occupationally exposed to storage mites had serum IgE specific for many potent allergens from T. putrescentiae. Persons who are exposed to stored product mites in occupational settings or by consumption of food containing these mites are therefore at risk of sensitization and allergic reaction. Increased awareness of sensitivity to this mite in the United States' population is needed.
  • Latex allergy diagnosis: in vivo and in vitro standardization of a natural rubber latex extract. [Journal Article]
    Allergy. 1997 Jan; 52(1):41-50.Turjanmaa K, Palosuo T, … Tran TX
  • For the diagnosis of IgE-mediated (immediate) hypersensitivity to natural rubber latex (NRL), skin prick testing with extracts of latex gloves has been widely used, but such extracts are difficult to standardize. The present study aimed to produce on an industrial scale an NRL extract from freshly collected NRL and to evaluate, calibrate, and standardize the extract by both in vivo and in vitro t…
  • Allergic rhinitis to thuja pollen. [Case Reports]
    Int Arch Allergy Immunol. 1996 May; 110(1):91-4.Guerin B, Kanny G, … Moneret-Vautrin DA
  • Allergy to pollen of Cupressaceae has been linked to pollens of Cupressus, Juniper and Cryptomeria. The authors report 2 cases of rhinitis and conjunctivitis induced by thuja, another member of the Cupressaceae family. Monosensitization to thuja pollen has been identified as the causal agent: (1) of a long-standing springtime rhinitis in 1 patient with negative skin tests and specific IgE titers …
  • Identification of IgE-binding egg white proteins: comparison of results obtained by different methods. [Journal Article]
    Int Arch Allergy Immunol. 1996 Jan; 109(1):50-7.Aabin B, Poulsen LK, … Barkholt V
  • The binding of IgE to egg white proteins was investigated for 34 sera from adults with a positive case history and/or positive RAST towards egg, and the impact of experimental conditions on IgE binding in commonly used methods was studied. Radioimmunoblotting after SDS-PAGE of both reduced and unreduced egg white extracts showed complex reaction patterns. The results were confirmed by crossed rad…
  • Heterogeneity of pollen proteins within individual Betula pendula trees. [Journal Article]
    Int Arch Allergy Immunol. 1995 Dec; 108(4):368-76.Hjelmroos M, Schumacher MJ, Van Hage-Hamsten M
  • To determine the variation of antigenic water soluble proteins in white birch (Betula pendula) pollen, extracts of pollen from different sides of individual trees were analyzed by isoelectric focusing (IEF), crossed immunoelectrophoresis, and crossed radioimmunoelectrophoresis. IgE-antibody-binding patterns were also studied in samples analyzed by IEF by probing with serum pooled from patients wi…
  • Allergen and protein content of latex gloves. [Journal Article]
    Ann Allergy. 1994 Oct; 73(4):315-20.Alenius H, Mäkinen-Kiljunen S, … Reunala T
  • CONCLUSIONS: In vitro assays showed considerable variation in the total protein and allergen contents of different latex glove brands. The amount of protein eluting from the gloves did not always correlate with their allergenicity in skin prick tests indicating that total protein measurement is not a sufficient method to monitor the allergenic properties of latex gloves.
  • Hypoglycaemia caused by atypical insulin antibodies in a patient with benign monoclonal gammopathy. [Case Reports]
    J Intern Med. 1993 Oct; 234(4):421-7.Arnqvist HJ, Halban PA, … von Schenck H
  • We describe a 48-year-old woman with recurrent severe hypoglycaemia apparently caused by a paraprotein with insulin-binding capacity. Very high fasting values were found for serum insulin (170 and > 250 mU l-1) as well as for proinsulin 125 pmol l-1 and an insulinoma was suspected. Hypoglycaemia developed after an oral glucose tolerance (OGTT) test but not during fasting for 48 h. Free insulin an…
  • Allergenicity of the mite, Blomia tropicalis. [Journal Article]
    J Allergy Clin Immunol. 1993 May; 91(5):1042-50.Arlian LG, Vyszenski-Moher DL, Fernandez-Caldas E
  • CONCLUSIONS: The results of this study indicated that BT contained multiple allergens of which most were species-specific. There was a limited amount of cross-reactivity between BT and the two common house dust mite species and the stored product mite. The amount of cross-reactivity appears to parallel the phylogenetic relatedness.
  • Comparison of the residual allergenic activity of six different hydrolyzed protein formulas. [Journal Article]
    J Pediatr. 1992 Nov; 121(5 Pt 2):S80-4.Wahn U, Wahl R, Rugo E
  • The residual allergenic activity of protein hydrolysates contained in marketed "hypoallergenic" infant formulas can be determined both by in vivo methods (skin prick tests, provocation tests) and by in vitro methods (radioallergosorbent test, radioallergosorbent inhibition, crossed radioimmunoelectrophoresis, basophil histamine release). In general, whey hydrolysates containing a high percentage …
  • Antigenic and allergenic analysis of psyllium seed components. [Journal Article]
    J Allergy Clin Immunol. 1992 Apr; 89(4):866-76.Arlian LG, Vyszenski-Moher DL, … Ritz HL
  • The outer portions (husk) of psyllium seeds are a concentrated source of natural fiber used in some bulk-fiber laxatives and cereals. They are known to elicit respiratory allergic reactions after inhalation or ingestion among sensitized individuals. Antigenic and allergenic characterization of three psyllium-seed fractions (husk, endosperm, and embryo) was conducted with crossed immunoelectrophor…
  • IgG and IgE antibody responses to cow dander and urine in farmers with cow-induced asthma. [Journal Article]
    Clin Exp Allergy. 1992 Jan; 22(1):83-90.Ylönen J, Mäntyjärvi R, … Virtanen T
  • Cow-asthmatic farmers' and negative control subjects' IgG and IgE antibody responses to bovine epithelial antigen (BEA) and urinary antigen (BUA) were studied by enzyme-linked immunosorbent assay (ELISA) and Western blotting. The anti-BEA IgE responses of 10 highly reactive sera were also studied by crossed radioimmunoelectrophoresis (CRIE). The relative amount of allergens common to both BEA and…
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