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22,417 results
  • Exploring taxonomic diversity and biogeography of the family Nemacheilinae (Cypriniformes). [Journal Article]
    Ecol Evol 2019; 9(18):10343-10353Chen W, Yang J, … Li X
  • Nemacheilidae, in the superfamily Cobitoidea, is comprised of many of morphologically similar fish species that occur in Eurasian water bodies. This large group shows inconsistencies between traditional morphological taxonomy and molecular phylogenetic data. We used mitochondrial genomes, recombinase-activating gene proteins 1 (RAG1) and the mitochondrial cytochrome c oxidase I gene (COI) to stud…
  • Cre-loxP Reporter Mouse Reveals Stochastic Activity of the Foxp3 Promoter. [Journal Article]
    Front Immunol 2019; 10:2228Bittner-Eddy PD, Fischer LA, Costalonga M
  • Mouse models that combine specific loxP-flanked gene sequences with Cre recombinase expressed from cell-regulated promoters have become important tools to investigate gene function. Critically however, expression of Cre recombinase may not always be restricted to the target cell or tissue of interest due to promiscuous activity of the driving promoter. Expression of Cre recombinase and, by extens…
  • An improved platform for functional assessment of large protein libraries in mammalian cells. [Journal Article]
    Nucleic Acids Res 2019Matreyek KA, Stephany JJ, … Fowler DM
  • Multiplex genetic assays can simultaneously test thousands of genetic variants for a property of interest. However, limitations of existing multiplex assay methods in cultured mammalian cells hinder the breadth, speed and scale of these experiments. Here, we describe a series of improvements that greatly enhance the capabilities of a Bxb1 recombinase-based landing pad system for conducting differ…
  • CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria. [Journal Article]
    Nat Microbiol 2019Wang G, Zhao Z, … Yoshikuni Y
  • It is generally believed that exchange of secondary metabolite biosynthetic gene clusters (BGCs) among closely related bacteria is an important driver of BGC evolution and diversification. Applying this idea may help researchers efficiently connect many BGCs to their products and characterize the products' roles in various environments. However, existing genetic tools support only a small fractio…
  • Myeloid cells activate iNKT cells to produce IL-4 in the thymic medulla. [Journal Article]
    Proc Natl Acad Sci U S A 2019Wang H, Breed ER, … Hogquist KA
  • Interleukin-4 (IL-4) is produced by a unique subset of invariant natural killer T (iNKT) cells (NKT2) in the thymus in the steady state, where it conditions CD8+ T cells to become "memory-like" among other effects. However, the signals that cause NKT2 cells to constitutively produce IL-4 remain poorly defined. Using histocytometry, we observed IL-4-producing NKT2 cells localized to the thymic med…
  • A CRISPR/Cas9-based genome editing system for Rhodococcus ruber TH. [Journal Article]
    Metab Eng 2019Liang Y, Jiao S, … Shen Z
  • Rhodococcus spp. are organic solvent-tolerant strains with strong adaptive abilities and diverse metabolic activities, and are therefore widely utilized in bioconversion, biosynthesis and bioremediation. However, due to the high GC-content of the genome (∼70%), together with low transformation and recombination efficiency, the efficient genome editing of Rhodococcus remains challenging. In this s…
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