- Direct Bioelectricity Generation from Sago Hampas by Clostridium beijerinckii SR1 Using Microbial Fuel Cell. [Journal Article]
- MMolecules 2019 Jun 28; 24(13)
- Microbial fuel cells offer a technology for simultaneous biomass degradation and biological electricity generation. Microbial fuel cells have the ability to utilize a wide range of biomass including …
Microbial fuel cells offer a technology for simultaneous biomass degradation and biological electricity generation. Microbial fuel cells have the ability to utilize a wide range of biomass including carbohydrates, such as starch. Sago hampas is a starchy biomass that has 58% starch content. With this significant amount of starch content in the sago hampas, it has a high potential to be utilized as a carbon source for the bioelectricity generation using microbial fuel cells by Clostridium beijerinckii SR1. The maximum power density obtained from 20 g/L of sago hampas was 73.8 mW/cm2 with stable cell voltage output of 211.7 mV. The total substrate consumed was 95.1% with the respect of 10.7% coulombic efficiency. The results obtained were almost comparable to the sago hampas hydrolysate with the maximum power density 56.5 mW/cm2. These results demonstrate the feasibility of solid biomass to be utilized for the power generation in fuel cells as well as high substrate degradation efficiency. Thus, this approach provides a promising way to exploit sago hampas for bioenergy generation.
- Zhang's guidelines vs WHO guidelines for diagnosing labour dystocia. [Letter]
- LctLancet 2019 Jun 25
- Characterization and properties of cellulose microfibers from water hyacinth filled sago starch biocomposites. [Journal Article]
- IJInt J Biol Macromol 2019 Jun 25; 137:119-125
- The cellulose microfibers (CMF) from water hyacinth (WH) fiber as a filler in sago starch (SS) biocomposites was investigated. The CMF was isolated by pulping, bleaching and acid hydrolysis methods. …
The cellulose microfibers (CMF) from water hyacinth (WH) fiber as a filler in sago starch (SS) biocomposites was investigated. The CMF was isolated by pulping, bleaching and acid hydrolysis methods. The addition of CMF in sago matrix was varied i.e. 0, 5, 10, 15 and 20 wt%. Biocomposites were made by using solution casting and glycerol as a plasticizer. The biocomposites were also determined by tensile test, FTIR, X-Ray, thermogravimetric, SEM, and soil burial tests. The results show that the SS15CMF sample has the highest tensile strength of 10.23 MPa than those other samples. Scanning Electron Microscope (SEM) images show that the strong interaction was formed between CMF WH and matrix. Fourier Transform Infra-red (FTIR) indicated that the functional group of biocomposites was a hydrophilic cluster. The addition of CMF WH in sago starch biocomposites lead to the moisture barrier, crystallinity, and thermal stability increased; it is due to the pure sago starch film was more rapidly degraded than its biocomposites.
- Perturbation of the immune cells and prenatal neurogenesis by the triplication of the Erg gene in mouse models of Down syndrome. [Journal Article]
- BPBrain Pathol 2019 Jun 17
- Some mouse models of Down syndrome (DS), including Ts1Cje mice, exhibit impaired prenatal neurogenesis with yet unknown molecular mechanism. To gain insights into the impaired neurogenesis, a transcr…
Some mouse models of Down syndrome (DS), including Ts1Cje mice, exhibit impaired prenatal neurogenesis with yet unknown molecular mechanism. To gain insights into the impaired neurogenesis, a transcriptomic and flow cytometry analysis of E14.5 Ts1Cje embryo brain was performed. Our analysis revealed that the neutrophil and monocyte ratios in the CD45-positive hematopoietic cells were relatively increased, in agreement with the altered expression of inflammation/immune-related genes, in Ts1Cje embryonic brain, whereas the relative number of brain macrophages was decreased in comparison to wild-type mice. Similar upregulation of inflammation-associated mRNAs was observed in other DS mouse models, with variable trisomic region lengths. We used genetic manipulation to assess the contribution of Erg, a trisomic gene in these DS models, known to regulation hemato-immune cells. The perturbed proportions of immune cells in Ts1Cje mouse brain were restored in Ts1Cje-Erg+/+/Mld2 mice, which are disomic for functional Erg but otherwise trisomic on a Ts1Cje background. Moreover, the embryonic neurogenesis defects observed in Ts1Cje cortex were reduced in Ts1Cje-Erg+/+/Mld2 embryos. Our findings suggest that Erg gene triplication contributes to the dysregulation of the homeostatic proportion of the populations of immune cells in the embryonic brain and decreased prenatal cortical neurogenesis in the prenatal brain with DS.
- Spray-dried immobilized lipase from Geobacillus sp. strain ARM in sago. [Journal Article]
- PPeerJ 2019; 7:e6880
- Sago starch is traditionally used as food especially in Southeast Asia. Generally, sago is safe for consumption, biodegradable, easily available and inexpensive. Therefore, this research was done to …
Sago starch is traditionally used as food especially in Southeast Asia. Generally, sago is safe for consumption, biodegradable, easily available and inexpensive. Therefore, this research was done to expand the potential of sago by using it as a support for enzyme immobilization. In this study, ARM lipase, which was isolated from Geobacillus sp. strain ARM, was overexpressed in Escherichia coli system and then purified using affinity chromatography. The specific activity of the pure enzyme was 650 U/mg, increased 7 folds from the cell lysate. The purified enzyme was immobilized in gelatinized sago and spray-dried by entrapment technique in order to enhance the enzyme operational stability for handling at high temperature and also for storage. The morphology of the gelatinized sago and immobilized enzyme was studied by scanning electron microscopy. The results showed that the spray-dried gelatinized sago was shrunken and became irregular in structure as compared to untreated sago powder. The surface areas and porosities of spray-dried gelatinized sago with and without the enzyme were analyzed using BET and BJH method and have shown an increase in surface area and decrease in pore size. The immobilized ARM lipase showed good performance at 60-80 °C, with a half-life of 4 h and in a pH range 6-9. The immobilized enzyme could be stored at 10 °C with the half-life for 9 months. Collectively, the spray-dried immobilized lipase shows promising capability for industrial uses, especially in food processing.
- Improving the quality of life during treatment in the Day hospital for early intervention in Psychiatric Hospital "Sveti Ivan". [Journal Article]
- PDPsychiatr Danub 2019; 31(Suppl 2):190-195
- CONCLUSIONS: Notwithstanding the limitations of this study, we may conclude that the findings are encouraging and may assist with development of more effective therapeutic approaches.
- Nutrient availability in sago bark and empty fruit bunch composts for the growth of water spinach and green mustard. [Journal Article]
- ESEnviron Sci Pollut Res Int 2019 May 31
- Sago bark (SB) and empty fruit bunch (EFB) are available abundantly as agricultural waste in Sarawak. This study was conducted to investigate the physicochemical characteristics of SB and EFB as comp…
Sago bark (SB) and empty fruit bunch (EFB) are available abundantly as agricultural waste in Sarawak. This study was conducted to investigate the physicochemical characteristics of SB and EFB as composting materials and used as a plant growth medium. The SB and EFB composts were prepared in a separate container by mixing chicken manure as compost accelerator and wood chips as a bulking agent in dry weight equivalent ratio (1:1:1). The maturity and stability of compost in 60-day composting periods were evaluated via physicochemical characterization of the composts in terms of pH, elemental content, total ash content, moisture content and nutrient analyses. The effect of the compost usage as growth medium was assessed towards water spinach and green mustard via seed germination and pot study. After 2 months, the colour of both composts was dark brown with an earthy smell. The acidic pH of the initial composting stage has changed into alkaline pH after 60 days of composting. Total NPK present in the SB and EFB composts were 0.96% and 1.21%, respectively. The germination index (GI) for the studied vegetables was above 100%, while the pot study showed that vegetables in compost media has higher growth compared to the control, after 14 days. SB and EFB are renewable waste which can be used as an excellent compost and able to improve the quality of the soil.
- Nationwide survey of fetal myelomeningocele in Japan: Background for fetal surgery. [Journal Article]
- PIPediatr Int 2019 May 22
- CONCLUSIONS: Gestational age at MMC diagnosis was late mid-gestation, therefore earlier detection is essential when considering fetal treatment of MMC in Japan. Although the survival rate was excellent, in three-quarters of isolated MMC cases ventriculoperitoneal shunt was required. Early detection and centralization of MMC cases at specialized centers should be considered.
- SSR identification and marker development for sago palm based on NGS genome data. [Journal Article]
- BSBreed Sci 2019; 69(1):1-10
- Sago palm (Metroxylon sagu Rottb.) is one of the most productive carbohydrate-producing crops. Unfortunately, only limited information regarding sago palm genetics is available. This study aimed to d…
Sago palm (Metroxylon sagu Rottb.) is one of the most productive carbohydrate-producing crops. Unfortunately, only limited information regarding sago palm genetics is available. This study aimed to develop simple sequence repeat (SSR) markers using sago palm NGS genomic data and use these markers to evaluate the genetic diversity of sago palm from Indonesia. De novo assembly of partial sago palm genomic data and subsequent SSR mining identified 29,953 contigs containing 31,659 perfect SSR loci and 31,578 contigs with 33,576 imperfect SSR loci. The perfect SSR loci density was 132.57/Mb, and AG, AAG and AAAT were the most frequent SSR motifs. Five hundred perfect SSR loci were randomly selected and used for designing SSR primers; 93 SSR primer pairs were identified. After synteny analysis using rice genome sequences, 20 primer pairs were validated using 11 sago palm accessions, and seven primers generated polymorphic alleles. Genetic diversity analysis of 41 sago palm accessions from across Indonesia using polymorphic SSR loci indicated the presence of three clusters. These results demonstrated the success of SSR identification and marker development for sago palm based on NGS genome data, which can be further used for assisting sago palm breeding in the future.
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- Some Properties of Chemical Cross-Linking Biohydrogel from Starch and Chitosan. [Journal Article]
- IJInt J Biomater 2019; 2019:1542128
- Chemical cross-linking was developed to prepare starch and chitosan-based hydrogels. First, the precursor of starch was synthesized through the reaction of carboxymethylation with sodium monochloroac…
Chemical cross-linking was developed to prepare starch and chitosan-based hydrogels. First, the precursor of starch was synthesized through the reaction of carboxymethylation with sodium monochloroacetate, and then chitosan was grafted by using methacrylic acid as cross-linker. In this research, sago and cassava starches were used and mixed with chitosan, and the effect of methacrylic acid concentration was investigated to determine the grafting parameters and hydrogel characteristics. Compared to native starch and carboxymethylated starch, hydrogels from both starches have high ability to swell and high capacity to absorb water and oil. The highest grafting yield, grafting efficiency, and monomer conversion were achieved by experiment using 0.550 g of methacrylic acid per g of CMS-chitosan mixture. These hydrogels have a good potency as biodegradable absorbents for pharmaceutical and industrial application.