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2,691 results
  • Osmium absorption after osmium tetroxide skin and eye exposure. [Journal Article]
    Basic Clin Pharmacol Toxicol. 2020 Jun 11 [Online ahead of print]Friedova N, Pelclova D, … Kohout P
  • Osmium tetroxide is a strong oxidizing agent used in electron microscopy. Eye exposure may cause severe burns, and after inhalation or ingestion damage to the respiratory or gastrointestinal tract occurs. Exposure to osmium and its compounds is extremely rare. We present a case of a 32-year-old female stained by 9 ml of 2% osmium tetroxide in acetone during an accident in the laboratory, with rar…
  • Comparison of Three Histological Techniques for Fat Emboli Detection in Lung Cetacean's Tissue. [Journal Article]
    Sci Rep. 2020 May 19; 10(1):8251.Arregui M, Fernández A, … de Quirós YB
  • Fat embolism is the mechanical blockage of blood vessels by circulating fat particles. It is frequently related to traumas involving soft tissues and fat-containing bones. Different techniques have been used for decades to demonstrate histologically fat emboli, being the extremely toxic post-fixation with osmium tetroxide one of the most used techniques in the last decades. In the present study, …
  • Mesoporous Octahedron-Shaped Tricobalt Tetroxide Nanoparticles for Photocatalytic Degradation of Toxic Dyes. [Journal Article]
    ACS Omega. 2020 Apr 14; 5(14):7823-7835.Sonkusare VN, Chaudhary RG, … Abdala AA
  • The present article reports a facile approach to fabrication of mesoporous octahedron-shaped tricobalt tetroxide nanoparticles (Co3O4 NPs) with a very narrow size distribution for eco-friendly remediation of toxic dyes. Co3O4 NPs were fabricated by a sol-gel process using cobalt chloride hexahydrate (CoCl2·6H2O) and monosodium succinate (C4H5O4Na) as a chelating/structure-directing agent and sodi…
  • A new technical approach for preparing frozen biological samples for electron microscopy. [Journal Article]
    Plant Methods. 2020; 16:48.Buchner O, Steiner P, … Lütz-Meindl U
  • CONCLUSIONS: The method presented allows preserving the ultrastructure of plant cells in the frozen state during cryo-fixation. The resulting high quality TEM-images represent an important step towards a better understanding of the consequences of extracellular ice formation on cellular ultrastructure. It has the potential to provide new insights into changes of organelle structure, identification of intracellular injuries during ice formation and may help to understand freezing and thawing processes in plant tissues. It may be combined with analytical TEM such as electron energy loss spectroscopy (EELS), X-ray analyses (EDX) and various other electron microscopic techniques.
  • Fat Embolism, Fat Embolism Syndrome and the Autopsy. [Review]
    Acad Forensic Pathol. 2019 Sep; 9(3-4):136-154.Milroy CM, Parai JL
  • Fat embolism is common following trauma and is a common autopsy finding in these cases. It may also be seen in non-traumatic cases and is seen in children as well as adults. In comparison fat embolism syndrome (FES) only occurs in a small number of trauma and non-trauma cases. Clinical diagnosis is based on characteristic clinical and laboratory findings. Fat embolism exerts its effect by mechani…
  • 2D and 3D immunogold localization on (epoxy) ultrathin sections with and without osmium tetroxide. [Journal Article]
    Microsc Res Tech. 2020 Jun; 83(6):691-705.Flechsler J, Heimerl T, … Klingl A
  • For nearly 50 years immunogold labeling on ultrathin sections has been successfully used for protein localization in laboratories worldwide. In theory and in practice, this method has undergone continual improvement over time. In this study, we carefully analyzed circulating protocols for postembedding labeling to find out if they are still valid under modern laboratory conditions, and in additio…
  • An adaptation of Twort's method for polychromatic staining of epoxy-embedded semithin sections. [Journal Article]
    Histochem Cell Biol. 2020 Feb; 153(2):121-127.Manskikh VN, Sheval EV
  • Epoxy-embedded semithin sections are useful for the analysis of cell and tissue organization, as well as for the processing of samples for transmission electron microscopy. Because only a very limited number of staining protocols have been developed for epoxy-embedded sections; semithin sections are used infrequently compared to conventional paraffin sections. Here, we describe a simple and repro…
  • Properties of a Steel Slag-Permeable Asphalt Mixture and the Reaction of the Steel Slag-Asphalt Interface. [Journal Article]
    Materials (Basel). 2019 Nov 02; 12(21)Liu W, Li H, … Xu P
  • Steel slag is an industrial solid waste with the largest output in the world. It has the characteristics of wear resistance, good particle shape, large porosity, etc. At the same time, it has good adhesion characteristics with asphalt. If steel slag is used in asphalt pavement, it not only solves the problem of insufficient quality aggregates in asphalt concrete, but can also give full play to th…
  • Unique features of the skin barrier in naked mole rats reflect adaptations to their fossorial habitat. [Journal Article]
    J Morphol. 2019 12; 280(12):1871-1880.Menon GK, Catania KC, … Mauldin EA
  • The stratum corneum (SC), the top layer of the epidermis, is the functional site of the skin barrier and serves to maintain hydration of the body by preventing water loss and thwarting the entrance of pathogens. The naked mole rat (NMR) (Heterocephalus glaber) is a rodent that resides in hypoxic underground tunnels in arid Africa. NMRs are not only hairless; their skin is devoid of glands and pai…
  • mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM. [Journal Article]
    Nat Methods. 2020 01; 17(1):55-58.Fu Z, Peng D, … Xu P
  • Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We des…
  • Nanopore device-based fingerprinting of RNA oligos and microRNAs enhanced with an Osmium tag. [Journal Article]
    Sci Rep. 2019 Oct 02; 9(1):14180.Sultan M, Kanavarioti A
  • Protein and solid-state nanopores are used for DNA/RNA sequencing as well as for single molecule analysis. We proposed that selective labeling/tagging may improve base-to-base resolution of nucleic acids via nanopores. We have explored one specific tag, the Osmium tetroxide 2,2'-bipyridine (OsBp), which conjugates to pyrimidines and leaves purines intact. Earlier reports using OsBp-tagged oligode…
  • Transient and intermediate carbocations in ruthenium tetroxide oxidation of saturated rings. [Journal Article]
    Beilstein J Org Chem. 2019; 15:1552-1562.Pedrón M, Legnani L, … Merino P
  • The ruthenium tetroxide-mediated oxidation of cyclopentane, tetrahydrofuran, tetrahydrothiophene and N-substituted pyrrolidines has been studied computationally by DFT and topological (analysis of the electron localization function, ELF) methods. In agreement with experimental observations and previous DFT calculations, the rate-limiting step of the reaction takes place through a highly asynchron…
  • Lead soaps formation and biodiversity in a XVIII Century wax seal coloured with minium. [Journal Article]
    Environ Microbiol. 2020 Apr; 22(4):1517-1534.Šoltys K, Planý M, … Pinzari F
  • A multidisciplinary approach was carried out in order to study the biodeterioration and the associated microbiome of a XVIII Century wax seal coloured with minium. A small wax seal fragment was observed by scanning electron microscopy combined with energy dispersive spectroscopy in non-destructive mode. The same object was analysed by Raman and Fourier-transform infrared spectroscopy. The identif…
  • Intersystem Crossings Drive Atmospheric Gas-Phase Dimer Formation. [Journal Article]
    J Phys Chem A. 2019 Aug 01; 123(30):6596-6604.Valiev RR, Hasan G, … Kurten T
  • High molecular weight "ROOR'" dimers, likely formed in the gas phase through self- and cross-reactions of complex peroxy radicals (RO2), have been suggested to play a key role in forming ultrafine aerosol particles in the atmosphere. However, the molecular-level reaction mechanism producing these dimers remains unknown. Using multireference quantum chemical methods, we explore one potentially com…
  • Hard and soft X-ray imaging to resolve human ovarian cortical structures. [Journal Article]
    J Synchrotron Radiat. 2019 Jul 01; 26(Pt 4):1322-1329.Pascolo L, Sena G, … Ricci G
  • Laboratory and synchrotron X-ray tomography are powerful tools for non-invasive studies of biological samples at micrometric resolution. In particular, the development of phase contrast imaging is enabling the visualization of sample details with a small range of attenuation coefficients, thus allowing in-depth analyses of anatomical and histological structures. Reproductive medicine is starting …
  • SEM/FIB Imaging for Studying Neural Interfaces. [Journal Article]
    Dev Neurobiol. 2019 Jun 22 [Online ahead of print]Henn I, Atkins A, … Mandel Y
  • Tissue and neural engineering for various regenerative therapies are rapidly growing fields. Of major interest is studying the complex interface between cells and various 3D structures by scanning electron microscopy with focused ion beam. Notwithstanding its unrivaled resolution, the optimal fixation, dehydration, and staining protocols of the samples while preserving the complex cell interface …
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