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(vitamin B7)
32,422 results
  • Development of a Double-Antibody Sandwich ELISA for Sensitive Detection of Yersinia pestis. [Journal Article]
    Microbiol Immunol 2019Choi SY, Rhie GE, Jeon JH
  • We developed a biotin-streptavidin-based sandwich enzyme-linked immunosorbent assay (ELISA) for the sensitive and specific detection of Y. pestis. In this assay, F1 capsular protein and Y. pestis were captured by anti-F1 mouse monoclonal antibody followed by detection with biotinylated-anti-F1 rabbit polyclonal antibody and horseradish peroxidase-conjugated streptavidin. The developed F1 ELISA co…
  • BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks. [Journal Article]
    Methods Mol Biol 2020; 2060:327-341Cheerathodi MR, Meckes DG
  • Herpes viruses are important human pathogens that cause a wide range of diseases from skin lesions to malignancies. Protein interactions drive many cellular events and mediate a number of biochemical pathways leading to different physiological outcomes. Protein interactions between viral proteins and host proteins play significant roles in viral entry, replication and suppression of host-immune r…
  • Structural insight into the carboxylesterase BioH from Klebsiella pneumoniae. [Journal Article]
    Biochem Biophys Res Commun 2019Wang L, Chen Y, … Xu Y
  • The BioH carboxylesterase which is a typical α/β-hydrolase enzyme involved in biotin synthetic pathway in most bacteria. BioH acts as a gatekeeper and blocks the further elongation of its substrate. In the pathogen Klebsiella pneumoniae, BioH plays a critical role in the biosynthesis of biotin. To better understand the molecular function of BioH, we determined the crystal structure of BioH from K…
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