Compared with conventional light microscopy (LM), confocal laser scan microscopy (CLSM) is characterized in part by increased light sensitivity and higher spatial resolution. These characteristics permit the detection and discrimination of minor changes in cells and tissues in a more specific, sensitive, and accurate way. Potential clinical applications related to these characteristics of CLSM are discussed. Preliminary data indicate that the higher light sensitivity of CLSM allows detection of low amounts of proto-oncogene mRNA which are minimally detectable by LM. Evaluation of Grimelius-stained sections of lung cancer by CLSM with antiflex permits detection of previously undetected granula. The higher spatial resolution of CLSM may prove to be essential for accurate assessment of nuclear shape, which is of prognostic importance in tumors of a variety of organs. When applied to cancer research and diagnosis or other relevant fields, CLSM allows improved insight in tumor cell biology and may lead to improved diagnostic pathology. Consequently, the use of CLSM could lead to more refined therapeutic indications.
Abstract
Journal Article
eng
20490074
Baak, J P., et al. "Potential Clinical Uses of Laser Scan Microscopy." Applied Optics, vol. 26, no. 16, 1987, pp. 3413-6.
Baak JP, Thunnissen FB, Oudejans CB, et al. Potential clinical uses of laser scan microscopy. Appl Opt. 1987;26(16):3413-6.
Baak, J. P., Thunnissen, F. B., Oudejans, C. B., & Schipper, N. W. (1987). Potential clinical uses of laser scan microscopy. Applied Optics, 26(16), 3413-6. https://doi.org/10.1364/AO.26.003413
Baak JP, et al. Potential Clinical Uses of Laser Scan Microscopy. Appl Opt. 1987 Aug 15;26(16):3413-6. PubMed PMID: 20490074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Potential clinical uses of laser scan microscopy.
AU - Baak,J P,
AU - Thunnissen,F B,
AU - Oudejans,C B,
AU - Schipper,N W,
PY - 2010/5/22/entrez
PY - 1987/8/15/pubmed
PY - 1987/8/15/medline
SP - 3413
EP - 6
JF - Applied optics
JO - Appl Opt
VL - 26
IS - 16
N2 - Compared with conventional light microscopy (LM), confocal laser scan microscopy (CLSM) is characterized in part by increased light sensitivity and higher spatial resolution. These characteristics permit the detection and discrimination of minor changes in cells and tissues in a more specific, sensitive, and accurate way. Potential clinical applications related to these characteristics of CLSM are discussed. Preliminary data indicate that the higher light sensitivity of CLSM allows detection of low amounts of proto-oncogene mRNA which are minimally detectable by LM. Evaluation of Grimelius-stained sections of lung cancer by CLSM with antiflex permits detection of previously undetected granula. The higher spatial resolution of CLSM may prove to be essential for accurate assessment of nuclear shape, which is of prognostic importance in tumors of a variety of organs. When applied to cancer research and diagnosis or other relevant fields, CLSM allows improved insight in tumor cell biology and may lead to improved diagnostic pathology. Consequently, the use of CLSM could lead to more refined therapeutic indications.
SN - 1559-128X
UR - https://www.unboundmedicine.com/prime/citation/20490074/Potential_clinical_uses_of_laser_scan_microscopy.
DB - PRIME
DP - Unbound Medicine
ER -
