TY - JOUR T1 - Imidazolones-based azo dye; design, synthesis, spectral characterization, biological evaluations, and combined in silico and in vitro analyses to assess the anticancer potential. AU - Tawfeeq,Muzhda Anwer, AU - Samad,Mohammed Kareem, Y1 - 2026/02/16/ PY - 2025/11/30/received PY - 2026/2/6/revised PY - 2026/2/9/accepted PY - 2026/3/10/medline PY - 2026/2/20/pubmed PY - 2026/2/19/entrez KW - Anti-cancer KW - Azo-imidazolone KW - Azo-oxazolone KW - Bioactivity KW - Docking KW - Synthesis SP - 109631 EP - 109631 JF - Bioorganic chemistry JO - Bioorg Chem VL - 173 N2 - A series of new azo-imidazolones-based azo dye were synthesized, and spectral characterization accomplished by using different spectrophotometric and spectrometric techniques such as FT-IR, UV-visible, 13C NMR, 1H NMR, DEPT-135, ESI-MS, and fluorescence spectroscopy. Biological evaluation included testing antibacterial properties under standard media, smart antibacterial screening tested by exposing the sample to UV light and determining the minimum inhibitory concentration (MIC), testing antifungal properties using a well diffusion assay, comparing antioxidant activity with ascorbic acid, and molecular docking studies targeting bacteria, fungi, and cancer cells. The cytotoxicity MTT assay on MCF-7 cells of synthesis compound C3 was also evaluated. The selectivity index (SI) was determined by comparing standard cell lines (WI-38/HFF. The P values of the hydrophobic nature of the synthesized compounds were calculated, and the compounds exhibited a hydrophobic nature with the potential to be permeated through the membrane Pfizer's criteria of intravenous injection were therefore used. All studies have been performed using appropriate controls and according to approved protocols. SN - 1090-2120 UR - https://www.unboundmedicine.com/prime/citation/41713102/Imidazolones-based_azo_dye DB - PRIME DP - Unbound Medicine ER -