Real-time monitoring and dynamic modulation of biological signals are crucial for understanding and reprogramming complex biological processes. However, modular sensing and actuation systems that respond to diverse signals remain largely unexplored. Here, we engineer a generalized biosensing and signaling reprogramming platform, circular RNA-protein circuit-mediated cargo release (CRMCR), enabling fast detection and precise rewiring of biological signals in a multicellular system. CRMCR is designed based on target signal-activated expression of a circular RNA scaffold, which mediates complementation of a split protease and facilitates the release of the reporter protein retained in the endoplasmic reticulum for signal production. We demonstrate that this platform allows detection of different biological signals including immunosuppressive factors, cytokines, and tumor-associated growth factors, with high sensitivity and fast response. Moreover, this design is further engineered as a reprogramming platform, allowing dynamic sensing and autonomous modulation of T cell activity in a multicellular system. This multifunctional platform provides a new direction for scalable detection and modulation of signals in a variety of biological scenarios.
Abstract
Journal Article
eng
42312881
Zhang, Miao, et al. "Generalized Biosensing and Signaling Reprogramming Using Circular RNA-Protein Circuit-Mediated Cargo Release." Analytical Chemistry, 2026.
Zhang M, Xu H, Zhao Z, et al. Generalized Biosensing and Signaling Reprogramming Using Circular RNA-Protein Circuit-Mediated Cargo Release. Anal Chem. 2026.
Zhang, M., Xu, H., Zhao, Z., Li, B. R., Zhang, Q., Wang, F., & Jiang, J. H. (2026). Generalized Biosensing and Signaling Reprogramming Using Circular RNA-Protein Circuit-Mediated Cargo Release. Analytical Chemistry. https://doi.org/10.1021/acs.analchem.6c02264
Zhang M, et al. Generalized Biosensing and Signaling Reprogramming Using Circular RNA-Protein Circuit-Mediated Cargo Release. Anal Chem. 2026 Jun 18; PubMed PMID: 42312881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Generalized Biosensing and Signaling Reprogramming Using Circular RNA-Protein Circuit-Mediated Cargo Release.
AU - Zhang,Miao,
AU - Xu,Huayu,
AU - Zhao,Zixia,
AU - Li,Bang-Rui,
AU - Zhang,Quan,
AU - Wang,Fenglin,
AU - Jiang,Jian-Hui,
Y1 - 2026/06/18/
PY - 2026/6/18/pubmed
PY - 2026/6/18/medline
PY - 2026/6/18/entrez
JF - Analytical chemistry
JO - Anal Chem
N2 - Real-time monitoring and dynamic modulation of biological signals are crucial for understanding and reprogramming complex biological processes. However, modular sensing and actuation systems that respond to diverse signals remain largely unexplored. Here, we engineer a generalized biosensing and signaling reprogramming platform, circular RNA-protein circuit-mediated cargo release (CRMCR), enabling fast detection and precise rewiring of biological signals in a multicellular system. CRMCR is designed based on target signal-activated expression of a circular RNA scaffold, which mediates complementation of a split protease and facilitates the release of the reporter protein retained in the endoplasmic reticulum for signal production. We demonstrate that this platform allows detection of different biological signals including immunosuppressive factors, cytokines, and tumor-associated growth factors, with high sensitivity and fast response. Moreover, this design is further engineered as a reprogramming platform, allowing dynamic sensing and autonomous modulation of T cell activity in a multicellular system. This multifunctional platform provides a new direction for scalable detection and modulation of signals in a variety of biological scenarios.
SN - 1520-6882
UR - https://www.unboundmedicine.com/prime/citation/42312881/Generalized_Biosensing_and_Signaling_Reprogramming_Using_Circular_RNA-Protein_Circuit-Mediated_Cargo_Release.
DB - PRIME
DP - Unbound Medicine
ER -
