Objective: To examine associations between lifestyle choices and academic performance, considering differences by gender and the potential influence of psychopathology. Participants: 1,447 first-year Canadian undergraduates. Methods: Exposures from the Fall 2021 U-Flourish survey included substance use, exercise, sleep, self-care, and screen time. Multivariable linear regressions estimated associations between exposures and cumulative GPA at year-end. Results: Males more commonly reported weekly binge drinking (17.7 vs. 12.2%, p = 0.01) and ≥7 h/day of leisure screen time (19.1 vs. 14.7%, p = 0.05), while females more commonly reported smoking tobacco/vaping in the past month (29.2 vs. 22.4%, p = 0.01). Poor sleep, daily smoking, and leisure screen time were independently associated with a lower average GPA. Multiple unhealthy lifestyle choices showed a dose-response association with lower GPA, particularly among females; adjustment for depressive symptoms partially attenuated these associations. Conclusion: Clustering of unhealthy lifestyle choices cumulatively undermines academic performance, underscoring the need for proactive, integrated health promotion targeting undergraduates.

Lung adenocarcinoma (LUAD) is the pathological type with the highest proportion and heterogeneity in lung cancer. NADPH oxidase is an important enzyme in the biological respiratory chain that plays a decisive role in cell growth, regulation, and apoptosis, which translating seven gene families, namely NOX1-5 and DUOX1-2. The current study aimed to investigate the expression, and prognostic and immune-related roles of this gene family in early and advanced LUAD. Gene expression was examined using the Oncomine™ database, prognostic value was analyzed by Kaplan-Meier analysis, expression of NOX2 and NOX4 in early and locally advanced LUAD was validated using RT-qPCR, and the correlation of infiltrating immune cells was validated by tumor immune estimation resources (TIMER). Over-expressed NOX4 was associated with lower prognosis; NOX2 was found to be under-expressed in LUAD. Both NOX2 and NOX4 expression was positively related to tumor purity and had positive correlation with infiltrating levels of immune cells in TIMER. The data reflected the potential association of NADPH oxidase in early and locally advanced lung adenocarcinoma. Our data suggested that the combination of NOX4 suppression, NOX2 depression, and immunotherapy would improve clinical outcome in these tumors.

Cognitive decline is a central feature of aging and a major determinant of disability and mortality. Understanding cognitive trajectories is essential for identifying individuals at risk and informing preventive strategies. In this study, we estimated transition probabilities across cognitive states and examined predictors of cognitive deficit and mortality among 1,413 community-dwelling older adults for nine years. Cognitive status was assessed at baseline and at two follow-up waves (2006, 2010, and 2015) using the Mini-Mental State Examination and categorized into four trajectories: stable, worsening, improving, or fluctuating. Transition probabilities and associated predictors were estimated using discrete-time Markov models via multinomial logistic regression, including baseline cognitive status, age, sex, frailty, multimorbidity, and depressive symptoms. During follow-up, 51.1% of participants experienced cognitive decline, 46.6% remained stable, 1.3% improved, and 1.1% fluctuated. Frailty increased the odds of both cognitive deficit (OR = 3.1; 95% CI 1.86-5.18) and mortality (OR = 4.2; 95% CI 2.66-6.69). Participants aged ≥ 80 years had higher risks of cognitive deficit (OR = 5.2; 95% CI 3.63-7.48) and death (OR = 11.9; 95% CI 8.30-17.21). Prior cognitive deficit predicted subsequent decline (OR = 7.5; 95% CI 4.77-11.77) and mortality (OR = 7.2; 95% CI 4.63-11.28), whereas female sex was associated with lower mortality risk (OR = 0.50; 95% CI 0.38-0.66). These findings indicate that cognitive decline in late life follows a progressive trajectory and highlight frailty and advanced age as key predictors of cognitive deterioration and mortality, emphasizing the importance of early identification and preventive strategies.

Transgender and gender diverse (TGD) youth experience disproportionately high rates of anxiety, depression, and suicide compared with their cisgender peers. Family relationships play a central role in shaping these outcomes, yet the specific influence of family acceptance on suicide risk during adolescent gender identity development remains insufficiently understood. This literature review examines evidence to evaluate how family acceptance, rejection, and gender-affirming behaviors affect suicidal ideation and suicide attempts among TGD adolescents younger than age 19. A structured search identified 15 peer-reviewed studies meeting the inclusion criteria. A narrative review identified four consistent themes: family rejection significantly increases suicide risks, family acceptance is one of the strongest protective factors against suicidality, gender-specific affirmation is uniquely protective beyond general emotional support, and family processes represent a modifiable intervention target. Findings underscore the critical role of the family system in suicide prevention among TGD youth. Enhanced clinical screening, proactive parental education, and development of structured family-based interventions are essential. Future research should prioritize longitudinal designs and demographic diversity to better understand how family acceptance evolves over time and influences developmental risk factors.

Nanoparticles (NPs) are alternative chemiluminescence (CL) luminophores to molecular luminophores and are strongly anticipated to extend molecular luminophore-tagged commercial CL immunoassays (CLIA) with wavebands beyond the eye-visible region and sensitivity beyond the pg/mL level. Herein, a surficial bond-involved repetitive excitation strategy for near-infrared CL with enhanced photons per luminophore is proposed by exploiting l-methionine (l-Met)-capped gold NPs (AuNPs) as luminophores. The surficial Au-S bonds of l-Met@AuNPs can be involved in the procedure of NP core excitation via oxidation, and bring out defect-involved CL around 830 nm. Because there are plenty of Au-S bonds on every AuNP core, l-Met@AuNPs can be repetitively excited over a hundred times and give off greatly enhanced CL photons per luminophore than all molecular CL luminophores. CL of l-Met@AuNPs/(NH4)2S2O8 can directly enable automatic near-infrared CLIA for myoglobin (MYO) determination on in vitro diagnostic instruments, with a limit of detection of 10 fg/mL (S/N = 3), which significantly surpasses the threshold of molecular luminophores. This Au-S bond-triggered AuNP CL strategy not only offers an alternative to molecular CL strategies with improved sensitivity and to II-VI NP CL strategies with fewer toxic concerns, but also indicates that exploiting the surficial bond energy of NPs could be an alternative to designing NPs with unique morphology, structure, and composition in the CL domain.